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Činidla pro nukleové kyseliny

Nucleic acid reagents form the foundation of genetic analysis and molecular biology, playing a pivotal role from cloning to gene expression studies. Delve into our collection featuring high-fidelity enzymes for end-point PCR, advanced reagents for isothermal amplification, and comprehensive kits for nucleic acid purification. For precision in quantitative assays, explore our selection of qPCR and RT-qPCR kits, primed for unparalleled accuracy. Our portfolio also includes next-generation sequencing reagents, offering cutting-edge solutions for genomic sequencing and personalized medicine advancements.

RT-PCR kits, Verso™

RT-PCR kits, Verso™

Supplier: Thermo Fisher Scientific

The Verso™ RT-PCR system combines a reverse transcription enzyme, improved priming options and optimised buffers to generate high yield and full length cDNA. With a variety of kit options for both 1- and 2-step RT-PCR reactions, Verso™ provides optimum flexibility and sensitivity in an easy to use format.

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Recombinant Taq DNA Polymerase

Recombinant Taq DNA Polymerase

Supplier: Cytiva

For PCR and other procedures requiring DNA polymerase activity at elevated temperatures.

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High MW genomic DNA isolation kits, MegaLong™

High MW genomic DNA isolation kits, MegaLong™

Supplier: G-Biosciences

The majority of genomic DNA extraction methods involve numerous physical manipulations, including mixing, pipetting, shaking, binding to resin, elution, which results in sheared DNA that may not be suitable for further analysis.

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MagBeads FastDNA™ Kit for Feces

MagBeads FastDNA™ Kit for Feces

Supplier: MP Biomedicals

The MagBeads FastDNA Kit for Feces allows thorough sample lysis and quick isolation of DNA from fecal samples. It is using magnetic beads with high DNA binding capacity for extraction of high yields of pure DNA. This is done manually using a magnetic rack or adapted to automated instruments for high throughput processing.

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Dye terminator removal, illustra™ AutoSeq™ G-50 columns

Dye terminator removal, illustra™ AutoSeq™ G-50 columns

Supplier: Cytiva

illustra™ AutoSeq™ G-50 columns are specifically designed to remove fluorescent dye-terminators from sequencing reactions prior to analysis on automated sequencers. Effective purification is essential for high quality sequencing results since residual dye-terminators can obscure data. illustra™ AutoSeq™ G-50 consists of MicroSpin™ columns containing Sephadex™ G-50, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artifacts on salt-sensitive automated sequencers.

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Magbeads fast circulating DNA kit, 192 preparations, MP Biomedicals

Magbeads fast circulating DNA kit, 192 preparations, MP Biomedicals

Supplier: MP Biomedicals

MagBeads Fast Circulating DNA Kit is intended for purification of high-quality circulating DNA (cfDNA) from cell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, Biochip analysis and NGS.

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DNA clean up, Sephadex® G-50 M DNA grade

DNA clean up, Sephadex® G-50 M DNA grade

Supplier: Cytiva

Sephadex™ G-50 M DNA Grade chromatography resin for purification of DNA fragments up to 20 bases in length from small molecules such as salts by size exclusion.

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Power supplies, Axygen®

Supplier: Corning

Axygen power supplies are ideal for most electrophoresis needs.

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Genomic DNA isolation from tissue, Axygen® AxyPrep™ Mag

Genomic DNA isolation from tissue, Axygen® AxyPrep™ Mag

Supplier: Corning

The AxyPrep™ Mag Tissue gDNA Kit utilises a magnetic bead-based technology to isolate genomic DNA from rodent tail tissues. DNA from other tissue types may be extracted with this protocol, but optimisation may be required.

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Sera-Xtracta genomic DNA kit

Sera-Xtracta genomic DNA kit

Supplier: Cytiva

Sera-Xtracta genomic DNA kit offers magnetic bead-based extraction and purification of genomic DNA from whole blood.

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Sera-Xtracta virus/pathogen kit

Sera-Xtracta virus/pathogen kit

Supplier: Cytiva

Sera-Xtracta virus/pathogen kit for high-throughput total nucleic acid (DNA/RNA) isolation from bacteria and viruses including adenovirus (type 14), influenza A (H3N2) and COVID-19.

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RNA Extraction Viral Kit

Supplier: BIOPREMIER

The RNA viral extraction kit is designed for the rapid simultaneous purification of viral RNA from cell–free samples, solid samples, swabs and transport media.

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illustra™ ExoProStar™ S kits, Cytiva

illustra™ ExoProStar™ S kits, Cytiva

Supplier: Cytiva

The ExoProStar™ S PCR and Sequence Reaction Clean-Up Kit updates existing enzymatic PCR clean up technology with Exonuclease I and Shrimp Alkaline Phosphatase to remove unincorporated primers and dNTPs. illustra ExoProStar™ S improves digestion efficiency with no degradation of the target PCR product. Sample remain completely intact and ready for immediate use in manual or automated processes. For maximum flexibility the enzymes are supplied in two separate tubes.

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SPINeasy® PCR Purification and Gel Extraction Kit, MP Biomedicals

SPINeasy® PCR Purification and Gel Extraction Kit, MP Biomedicals

Supplier: MP Biomedicals

SPINeasy PCR purification and gel extraction Kit is a silica-membrane spin-column kit that enables quick and convenient DNA clean up from various enzymatic reactions, such as PCR and restriction digestion, as well as isolation and purification of DNA fragments from agarose gel electrophoresis.

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SPINeasy® Plasmid Purification, MidiPrep Kit, MP Biomedicals

SPINeasy® Plasmid Purification, MidiPrep Kit, MP Biomedicals

Supplier: MP Biomedicals

SPINeasy Plasmid Midiprep kit is a silica-membrane spin-column kit for the purification of high-copy and low-copy number plasmid DNA from 25 to 50 ml of transformed bacterial (E.coli) culture.

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SPINeasy® DNA Purification Kit, MP Biomedicals

SPINeasy® DNA Purification Kit, MP Biomedicals

Supplier: MP Biomedicals

Humic acids, heavy metals, heme are the most notorious PCR inhibitors. This could be due to non-optimised DNA purification procedures which often co-purify inhibitors and lead to false negative results on downstream applications.

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illustra™ Hot Start mix, Ready-To-Go™

illustra™ Hot Start mix, Ready-To-Go™

Supplier: Cytiva

illustra™ Hot Start Mix, Ready-To-Go™ is a pre-formulated and pre-dispensed, freeze-dried PCR reagent mix, including high quality PuReTaq™ DNA polymerase and a hot start activator protein, for increased specificity and reproducibility of PCR amplifications. The Ready-To-Go™ beads are provided in either 0,5 or 0,2 ml tubes that are compatible with most thermal cyclers.

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illustra™ MicroSpin™ S-300 HR columns

illustra™ MicroSpin™ S-300 HR columns

Supplier: Cytiva

illustra™ MicroSpin™ S-300 HR columns designed for rapid purification of PCR products (>100 bp) from unincorporated primers (<20-mers) and nucleotides using spin-column chromatography.

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Sera-Xtracta Cell-Free DNA Kit

Sera-Xtracta Cell-Free DNA Kit

Supplier: Cytiva

Sera-Xtracta Cell-Free DNA Kit for the efficient extraction and purification of cell-free DNA (cfDNA) from plasma.

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ABsolute™ qPCR Mixes

Supplier: Thermo Fisher Scientific

ABsolute™ qPCR Mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers. Versions are available for use with probes or with SYBR® Green I included, and with or without ROX or Fluorescein passive reference dyes.

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Genomic DNA extraction kits, plant gDNA kit, Axygen® AxyPrep™ Mag

Genomic DNA extraction kits, plant gDNA kit, Axygen® AxyPrep™ Mag

Supplier: Corning

Axygen® AxyPrep™ Mag Plant gDNA Kit is a magnetic bead-based purification system that allows rapid and reliable isolation of high quality gDNA from a wide variety of plant samples. Up to 96 samples (50 mg fresh tissue per sample or 15 mg of dried tissue per sample) can be processed in less than one hour. The Axygen® AxyPrep™ Mag Plant gDNA Kit eliminates polysaccharides, phenolic compounds and enzyme inhibitors with no organic extractions. This kit is adaptable for use on major liquid handling workstations for high throughput processing.

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RNAspin Mini Kits

RNAspin Mini Kits

Supplier: Cytiva

RNAspin Mini RNA Isolation Kit is a complete RNA purification kit designed for rapid extraction of high-quality total RNA from a wide range of sample types.

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ABsolute™ qPCR Capillary Mixes

Supplier: Thermo Fisher Scientific

ABsolute™ qPCR Capillary Mixes contain an optimised reaction buffer specifically designed to provide the highest possible sensitivity, specificity and consistent end-point values in real time instruments that use glass capillaries, such as the Roche LightCycler®. The 2X mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes.

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Magbeads FastDNA/RNA kit for virus, 192 preparations, MP Biomedicals

Magbeads FastDNA/RNA kit for virus, 192 preparations, MP Biomedicals

Supplier: MP Biomedicals

MagBeads FastDNA/RNA Kit for Virus is intended for extracting total viral nucleic acid from cell-free/low-cell content biological samples such as body fluids, serum, plasma, soaking solution, tissue homogenate, and culture supernatant.

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Magbeads FastDNA kit for blood, 96 preparations, MP Biomedicals

Magbeads FastDNA kit for blood, 96 preparations, MP Biomedicals

Supplier: MP Biomedicals

MagBeads FastDNA® Kit for Blood is intended for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.

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PCR clean-up reagent, Sera-Mag™ select

PCR clean-up reagent, Sera-Mag™ select

Supplier: Cytiva

Sera-Mag Select PCR clean-up, size selection reagent combines the exceptional binding characteristics of Sera-Mag carboxyl speedbeads with an optimised binding solution in a ready to use formulation. The DNA size range isolated from the process can be tailored to suit the user’s end requirements by adjusting the amount of reagent that is mixed with a fixed volume of sample.

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DNA clean up, Sephadex® G-100 DNA grade

DNA clean up, Sephadex® G-100 DNA grade

Supplier: Cytiva

Sephadex™ G-100 DNA Grade is ideal for use in preparing spin columns for DNA purification.

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DNA extraction, illustra™ Nucleon™ PhytoPure™

DNA extraction, illustra™ Nucleon™ PhytoPure™

Supplier: Cytiva

Nucleon™ PhytoPure™ enables rapid chloroform extraction of high-quality, high molecular weight genomic DNA from plant and fungal samples with efficient removal of protein as well as polysaccharides.

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Genomic DNA purification kits, Fast DNA™ SPIN kit for Soil, MP Biomedicals

Supplier: MP Biomedicals

The FastDNA™ SPIN Kit for Soil is designed to efficiently isolate bacterial, fungi, plant and animal genomic DNA from soil and environmental samples.

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illustra™ MicroSpin™ G-25 columns

illustra™ MicroSpin™ G-25 columns

Supplier: Cytiva

For rapid buffer exchange/desalting of PCR products and other DNAs in a volume of 10 to 100 μl using spin column chromatography.

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