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Supplier: Thermo Fisher Scientific

Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mix is a ready to use 2X reaction mix that includes DreamTaq™ Hot Start DNA Polymerase, DreamTaq™ buffer, magnesium, and dNTPs. The master mix allows easy reaction setup with fewer pipetting steps, with only primers, template, and water needing to be added. The green master mix includes density reagent and two tracking dyes for convenient direct loading of PCR products on a gel.

Supplier: Thermo Fisher Scientific

Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, Taq DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3’-end of PCR products. Native Taq DNA polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in E.coli.

Supplier: AAT Bioquest

TAQuest™ qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR compatible with TaqMan gene expression assays.

Supplier: AAT Bioquest

TAQuest™ FAST qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.

Supplier: AAT Bioquest

TAQuest™ qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.

Supplier: AAT Bioquest

TAQuest™ qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.

Supplier: AAT Bioquest

TAQuest™ qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR compatible with TaqMan gene expression assays.

Supplier: AAT Bioquest

JUN dye qPCR Calibration Solution supplied as a 10000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ 7 systems.

Supplier: AAT Bioquest

VIC dye qPCR Calibration Solution supplied as a 10000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ 7 systems.

Supplier: MP Biomedicals

The FastRNA™ SPIN Kit for Yeast quickly and efficiently isolates high-quality, total RNA from tough-to-lyse yeast strains, fungi, and algae in approximately 15 minutes using a specialised Lysing Matrix (Yttria-stabilised zirconium oxide, 0,5 mm) for cell lysis and SPIN columns for the purification process.

Supplier: Cytiva

PCR nucleotide mix dNTP set (2 mM, 10 mM, or 25 mM each A,C,G,T) for amplification, dideoxy sequencing, labelling, mutagenesis, cDNA synthesis, and expression profiling.

Supplier: MP Biomedicals

Set consists of 100 mM aqueous solutions of dATP, dCTP, dGTP and dTTP each supplied in a separate vial.

Supplier: Thermo Fisher Scientific

The aLICator™ LIC Cloning and Expression System is designed for fast and efficient ligation independent cloning and tight regulation of gene expression in E. coli. The plate bacterial expression vectors are designed for high levels of target protein expression in concert with minimal basal (uninduced) expression, which permits expression of proteins that are toxic to E. coli cells.

Supplier: SGI - DNA Synthetic Genomics

The Gibson Assembly® Ultra reaction occurs in two steps using the addition of two master mixes in two sequential steps. In the first step, the GA Ultra Master Mix A (2X) creates single-strand DNA 5’ overhangs by chewing back DNA from the 3’ end. This reaction is cooled under conditions that allow for annealing of complementary overlap regions. Next, the Gibson Assembly® Ultra Master Mix B (2X) is added. During this step, nucleotides are incorporated into the construct to fill in the gaps in the annealed DNA fragments and nicks are sealed to create a contiguous DNA construct.

Supplier: Cytiva

dNTPs designed for applications requiring high purity such as amplification, dideoxy sequencing, labeling, mutagenesis, cDNA synthesis, and expression profiling.

Supplier: Cytiva

Band compressions are ambiguous band patterns on sequencing gels caused by stable intra-strand secondary structures that are not fully denatured during electrophoresis. Their occurrence can be reduced by substituting nucleotide analogues in the sequencing mixes. 7-Deaza dGTP (c⁷dGTP) can be used to resolve G-band compressions.

Supplier: SGI - DNA Synthetic Genomics

The Gibson Assembly® HiFi 1 Step Kit provides a simple and effective method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method allows researchers to achieve complex assembly of large DNA constructs, with multiple inserts, in 1 hour.

Supplier: Abnova

The BSA I restriction endonuclease is a restriction endonuclease BsaI that can recognize specific sites expressed by Escherichia coli recombinantly. Restriction endonucleases are widely used in various fields such as gene positioning and cloning. This enzyme cleaves DNA rapidly for efficient gene linearization.

Supplier: Abnova

The restriction endonuclease is a restriction endonuclease BspQ I that can recognize specific sites and is produced under GMP standards and expressed recombinantly in Escherichia coli. Restriction endonucleases are widely used in various fields such as gene positioning and cloning. This enzyme cleaves DNA rapidly for efficient gene linearization.

Supplier: Biotium

A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.

Supplier: G-Biosciences

GET™ Plant DNA Template is a spin column format kit suitable for the preparation of DNA templates from plant samples. The isolated genomic DNA is suitable for PCR and other applications. GET™ Plant DNA Template purifies plant genomic DNA free from plant impurities known to inhibit downstream applications. The high yield, extracted DNA is normally >50 kb in size. The yield is ~30 µg DNA per preparation, depending on the plant source and quantity used.

Supplier: G-Biosciences

GET™ Plasmid DNA Miniprep Kit isolates high quality plasmid DNA from 1 to 5 ml E. coli cultures. The kit utilises an enhanced DNA binding column to produce high yields of plasmid.

Supplier: G-Biosciences

OmniPrep™ for Fungi Kit isolates high quality genomic DNA from fungal samples.If used according to the protocols this kit purifies DNA from 1 to 2 g fungal tissues.

Supplier: G-Biosciences

Arrest™ Extraction Buffer is an optimised combination of various chaotropic agents and RNase inhibitors, which inhibits RNase rapidly. It may be used in conjunction with any RNA extraction method, including extractions based on phenol, chloroform and other organic solvents and detergents.

Supplier: G-Biosciences

The EZ Yeast™ Plasmid Prep Kit is designed for the rapid isolation of plasmids from yeast patches or yeast grown in small liquid cultures. The kit is adapted from the alkaline lysis of E. coli, by providing modified alkaline lysis buffers and LongLife™ Zymolyase®, a highly stable enzyme.

Supplier: Cytiva

TempliPhi™ Kits efficiently prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi™ uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30 °C, enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi™ protocol requires less than 20 minutes of hands-on time to amplify 96 samples from bacterial colonies.

Supplier: Cytiva

illustra Ready-To-Go GenomiPhi V3 DNA Amplification Kit offers highly efficient and representative whole-genome amplification with 12 to 20 μg yield from nanogram amounts of DNA sample.

Supplier: G-Biosciences

Pfu DNA Polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerases. It has a molecular weight of 90 kDa and can amplify DNA targets up to 2 kb. The elongation velocity is 0,2~0,4 kb/min (70~75 °C). Pfu DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. This means that Pfu DNA Polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA Polymerase results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis.

Supplier: Thermo Fisher Scientific

GeneJET™ Plant RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from a wide variety of plant species and tissue types.

Supplier: G-Biosciences

G-Biosciences' GeneCAPSULE™ is an electro-elution tool for rapid, nearly 90% recovery of PCR products, DNA fragments and proteins from agarose and polyacrylamide gels