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Činidla pro nukleové kyseliny

Nucleic acid reagents form the foundation of genetic analysis and molecular biology, playing a pivotal role from cloning to gene expression studies. Delve into our collection featuring high-fidelity enzymes for end-point PCR, advanced reagents for isothermal amplification, and comprehensive kits for nucleic acid purification. For precision in quantitative assays, explore our selection of qPCR and RT-qPCR kits, primed for unparalleled accuracy. Our portfolio also includes next-generation sequencing reagents, offering cutting-edge solutions for genomic sequencing and personalized medicine advancements.

Nucleic acid extraction, MPure™ Viral nucleic acids extraction kits, MP Biomedicals

Nucleic acid extraction, MPure™ Viral nucleic acids extraction kits, MP Biomedicals

Supplier: MP Biomedicals

MPure™ Viral Nucleic Acids Extraction Kit is used with the MPure-12 instrument for extraction of Viral DNA or RNA from human biological specimens such as serum, plasma, and other cell-free fluids.

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Sequencing reaction purification, illustra™ AutoScreen 96 Well Plates

Sequencing reaction purification, illustra™ AutoScreen 96 Well Plates

Supplier: Cytiva

AutoScreen 96 well plate consists of a 96-well filter plate containing DNA grade Sephadex™ G-50 for purification of sequencing reactions prior to analysis on MegaBACE 1000 and can be used for other size exclusion applications.

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Phusion® High-Fidelity DNA Polymerase

Phusion® High-Fidelity DNA Polymerase

Supplier: FINNZYMES REAGENTS

Phusion® High Fidelity DNA Polymerase generates PCR products with an accuracy and speed unattainable with a single enzyme, even on the most difficult templates. The structure and characteristics of Phusion® High Fidelity DNA Polymerase make it a superior choice for cloning, and sets the standard for PCR performance with an error rate 50-fold lower than that of Taq DNA polymerase, and 6-fold lower than that of Pfu DNA polymerase. In addition, Phusion® High Fidelity DNA Polymerase possesses processivity 10-fold greater than Pfu DNA polymerase.

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Phusion® High-Fidelity PCR Kit

Phusion® High-Fidelity PCR Kit

Supplier: FINNZYMES REAGENTS

Phusion® High-Fidelity PCR Kit offers extreme performance for all major PCR applications. Incorporating Phusion® DNA polymerase, the Phusion® High-Fidelity PCR Kit contains all the reagents necessary, including lambda DNA control template and primers for 1,3 kb and 10 kb amplicons. The template amount is sufficient for performing 20 control reactions in 50 μl volume or 50 control reactions in 20 μl volume.

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Phusion® Flash high-fidelity PCR master mix

Phusion® Flash high-fidelity PCR master mix

Supplier: FINNZYMES REAGENTS

Phusion® Flash High-Fidelity PCR Master Mix is a 2X master mix enabling the use of extremely short PCR protocols without compromising either the fidelity or the yield in the reaction. The master mix utilises Phusion® Flash II DNA Polymerase, a proofreading DNA polymerase modified from Phusion® Hot Start II High-Fidelity DNA Polymerase. The fidelity of the Phusion® Flash II DNA Polymerase is 25-fold greater than Taq.

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DyNAmo™ ColorFlash SYBR® green QPCR kits

DyNAmo™ ColorFlash SYBR® green QPCR kits

Supplier: FINNZYMES REAGENTS

DyNAmo™ qPCR Kits offer excellent performance in detection and quantitation of DNA and RNA sequences from various sources. The DyNAmo™ qPCR kit family features optimised kits for fast and conventional qPCR. Kits utilising either SYBR® Green or probe chemistries for various platforms are available. All DyNAmo™ qPCR kits are provided as convenient 2X master mixes - only template and primers need to be added.

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DreamTaq™ Green DNA Polymerase

DreamTaq™ Green DNA Polymerase

Supplier: Thermo Fisher Scientific

DreamTaq™ Green DNA Polymerase is a combination of DreamTaq™ DNA Polymerase and 10X DreamTaq™ Green Buffer. DreamTaq™ DNA Polymerase is an enhanced Taq DNA polymerase optimised for high throughput PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase.
DreamTaq™ Green DNA Polymerase incorporates modified nucleotides, but is inhibited by dUTP. The 10X DreamTaq™ Green Buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with PCR performance and is compatible with downstream applications such as DNA sequencing,
phosphorylation, ligation and restriction digestion. For applications that require PCR product analysis by absorbance or fluorescence excitation, it is recommended either to use colourless 10X DreamTaq™ Buffer or purify the PCR product prior to analysis.

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Maxima™ SYBR® green QPCR master mix (2X), with ROX

Maxima™ SYBR® green QPCR master mix (2X), with ROX

Supplier: Thermo Fisher Scientific

The Maxima™ SYBR® Green qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase, SYBR® Green dye I and dNTPs in an optimised PCR buffer. ROX solution is provided in a separate vial for use with machines that require ROX as a passive reference dye. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ SYBR® Green qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

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PCR buffers

Supplier: Thermo Fisher Scientific

Thermo Scientific 10X Taq Buffers are ready-to-use buffers for PCR using Taq DNA Polymerases (both recombinant and native). Available in different compositions and also without detergents.

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Master mixes, green multiplex, Phusion® U

Master mixes, green multiplex, Phusion® U

Supplier: FINNZYMES REAGENTS

Phusion® U green multiplex PCR master mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.

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Phusion® green hot start II high-fidelity PCR master mix

Phusion® green hot start II high-fidelity PCR master mix

Supplier: FINNZYMES REAGENTS

Phusion® green hot start II high-fidelity PCR master mix is a convenient 2X mix designed to minimise the number of pipetting steps. The master mix contains Phusion Hot Start II DNA Polymerase, nucleotides and optimised reaction buffer including MgCl₂. The buffer also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel.

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Master mixes, hot start, Phusion® U

Supplier: FINNZYMES REAGENTS

Phusion® U hot start PCR master mix is a novel engineered high fidelity enzyme developed using fusion technology.

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Fast RNA™ Pro green kit, MP Biomedicals

Fast RNA™ Pro green kit, MP Biomedicals

Supplier: MP Biomedicals

FastRNA® Pro Green Kit is used to isolate total RNA from any type of plant and animal tissue or cultured cells. Extracted RNA is suitable for RT-PCR analysis and other downstream applications.

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MPure-32™ aNAP Nucleic Acid Extraction System, MP Biomedicals

Supplier: MP Biomedicals

MPure-32™ aNAP System is a fully automated magnetic bead-based nucleic acid extraction system. It can process up to 32 samples simultaneously within a short period of time (around 40 to 60 minutes). Besides its capability to extract nucleic acid with high purity and yield, MPure-32™ aNAP System is easy to use; this eliminates the risk of human error and cross-contamination.

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TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX*

Supplier: AAT Bioquest

TAQuest™ FAST qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.

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TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX*

Supplier: AAT Bioquest

TAQuest™ FAST qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.

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TAMRA Dye qPCR Calibration Solution *10000X*

TAMRA Dye qPCR Calibration Solution *10000X*

Supplier: AAT Bioquest

TAMRA dye qPCR Calibration Solution supplied as a 10000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ 7 systems

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DNA extraction kits, MPure™ Plant DNA extraction kit, MP Biomedicals

DNA extraction kits, MPure™ Plant DNA extraction kit, MP Biomedicals

Supplier: MP Biomedicals

MPure™ Plant DNA Extraction Kit is used with the MPure-12™ Automated Nucleic Acid Purification System for extraction of genomic DNA from plant (leaf, seeds and spores) and fungal tissues. Up to 100 mg of tissue can be used for purification.

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Taq DNA polymerase (native, with BSA)

Supplier: Thermo Fisher Scientific

Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, Taq DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Native Taq DNA polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in E.coli. Native Taq DNA polymerase stabilised with BSA is often the best choice when amplifying DNA samples of lower purity, e.g. genomic DNA from mouse tail.

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dNTP mix

dNTP mix

Supplier: Thermo Fisher Scientific

A combined mixture of  dATP, dCTP, dGTP and dTTP containing 5 mM of each dNTP. Applications include PCR, RT-PCR, DNA labelling, and site-directed mutagenesis.

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Tissue direct PCR master mix, Phire™

Tissue direct PCR master mix, Phire™

Supplier: FINNZYMES REAGENTS

Phire™ Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects. The master mix containing Phire™ Hot Start II DNA polymerase is specially formulated to perform PCR in the presence of different animal tissue-derived inhibitors, such as collagen, melanin and eumelanin (hair, skin) or myoglobin (muscle).

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DNA purification kits, GeneClean® for Ancient DNA™ Kit, MP Biomedicals

DNA purification kits, GeneClean® for Ancient DNA™ Kit, MP Biomedicals

Supplier: MP Biomedicals

The GeneClean® for Ancient DNA™ Kit is ideal for purification of fragmented or damaged DNA from aged or preserved samples. It is designed for isolation of up to 20 μg DNA from bone, preserved tissue, soil organisms, or animal by-products.

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Plasmid purification, MiniPrep Express™ Matrix, MP Biomedicals

Plasmid purification, MiniPrep Express™ Matrix, MP Biomedicals

Supplier: MP Biomedicals

MiniPrep Express™ Matrix is designed for rapid purification of plasmid DNA. It is an ideal tool for screening hundreds of recombinant clones in record time using restriction enzyme analysis and PCR.

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DNA clean up kits, GeneClean® Turbo Kits, MP Biomedicals

DNA clean up kits, GeneClean® Turbo Kits, MP Biomedicals

Supplier: MP Biomedicals

GeneClean® Turbo Kit is used for purification of DNA fragments of sizes 0,1 kb to 300 kb from TAE or TBE buffered agarose gels, PCR reactions and other enzymatic solutions. Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.

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DNA purification kits, GeneClean® III Kit, MP Biomedicals

DNA purification kits, GeneClean® III Kit, MP Biomedicals

Supplier: MP Biomedicals

The GeneClean® III Kit is used to purify fragments of DNA from 200 bp to 20 kb from solutions and agarose gels.

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SPINeasy® DNA Kit for Yeast

Supplier: MP Biomedicals

SPINeasy® DNA Kit for Yeast is a high-performance genomic DNA (gDNA) extraction kit which is developed based on silica-membrane spin-column technology. This kit enables quick isolation of gDNA from yeast cells, typically in less than 30 minutes.

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Master mixes, multiplex, Phusion® U

Master mixes, multiplex, Phusion® U

Supplier: FINNZYMES REAGENTS

Phusion® U multiplex PCR master mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.

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DNA polymerases, Phusion® hot start II high-fidelity PCR master mix

DNA polymerases, Phusion® hot start II high-fidelity PCR master mix

Supplier: FINNZYMES REAGENTS

Phusion® high-fidelity DNA polymerases set a gold standard for high performance PCR.

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DreamTaq™ PCR master mix (2X)

DreamTaq™ PCR master mix (2X)

Supplier: Thermo Fisher Scientific

DreamTaq™ PCR Master Mix (2X) is a ready to use solution containing DreamTaq™ DNA polymerase, optimised DreamTaq™ buffer, MgCl₂ and dNTPs. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set-up. DreamTaq™ DNA polymerase is an enhanced Taq polymerase optimised for high throughput PCR applications. It ensures robust amplification of PCR products up to 6 kb from genomic DNA and up to 20 kb from viral DNA.

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DreamTaq™ Green PCR Master Mix (2X)

DreamTaq™ Green PCR Master Mix (2X)

Supplier: Thermo Fisher Scientific

DreamTaq Green PCR Master Mix (2X) is a ready to use solution containing DreamTaq™ DNA polymerase, optimised DreamTaq Green buffer, MgCl2 and dNTPs. The master mix is supplemented with two tracking dyes and a density reagent that allows for direct loading of the PCR product on a gel. The dyes in the master mix do not interfere with PCR performance and are compatible with downstream applications, such as DNA sequencing, ligation and restriction digestion. The master mix retains all features of DreamTaq DNA polymerase. It is capable for robust amplification up to 6 kb from genomic DNA and up to 20 kb from viral DNA.

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