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Činidla pro nukleové kyseliny

Nucleic acid reagents form the foundation of genetic analysis and molecular biology, playing a pivotal role from cloning to gene expression studies. Delve into our collection featuring high-fidelity enzymes for end-point PCR, advanced reagents for isothermal amplification, and comprehensive kits for nucleic acid purification. For precision in quantitative assays, explore our selection of qPCR and RT-qPCR kits, primed for unparalleled accuracy. Our portfolio also includes next-generation sequencing reagents, offering cutting-edge solutions for genomic sequencing and personalized medicine advancements.

dNTP

dNTP

Supplier: VWR Chemicals

dNTP mixy a dNTP sady v kvalitě pro molekulární biologii vhodné k okamžitému použití. dNTP mix je připraven pro úsporu času stráveného manuálním zpracováváním a redukuje pravděpodobnost kontaminace omezením pipetování. Roztoky dNTP jsou též dostupné v sadách po čtyřech jednotlivých 100 mm dNTP. Oboje jsou vhodné pro použití pro PCR, značení DNA a sekvenační procesy.

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Peqlab peqGOLD, Deoxynucleotide Triphosphate (Solution)

Peqlab peqGOLD, Deoxynucleotide Triphosphate (Solution)

Supplier: VWR Peqlab

Individually packaged deoxynucleotide triphosphates manufactured to the highest quality levels.

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VWR®, dTTP solution

VWR®, dTTP solution

Supplier: VWR Chemicals

Deoxyribonucleotides are the foundation of DNA and it takes thousands of these nucleotides to make up DNA.

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TriLink® Uridine-5'-Triphosphate

TriLink® Uridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

This nucleotide consists of an unmodified base, a ribose, and a 5′ triphosphate group for synthesis of RNA molecules.

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TriLink® 2',3'-Dideoxycytidine-5'-O-(1-Thiotriphosphate)

TriLink® 2',3'-Dideoxycytidine-5'-O-(1-Thiotriphosphate)

Supplier: TriLink BioTechnologies

2',3'-Dideoxycytidine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddCTP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.

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TriLink® 2'-Deoxyguanosine-5'-O-(1-Thiotriphosphate)

TriLink® 2'-Deoxyguanosine-5'-O-(1-Thiotriphosphate)

Supplier: TriLink BioTechnologies

TriLink® 2'-Deoxyguanosine-5'-O-(1-Thiotriphosphate)

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TriLink® N1-Propylpseudouridine-5'-Triphosphate

TriLink® N1-Propylpseudouridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

TriLink® N1-Propylpseudouridine-5'-Triphosphate

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Fragment selection kits, Axygen® AxyPrep™ FragmentSelect-I Kit

Fragment selection kits, Axygen® AxyPrep™ FragmentSelect-I Kit

Supplier: Corning

The AxyPrep™ FragmentSelect Kits utilise a paramagnetic bead technology for quick high throughput optimised DNA size selection suitable for various next generation sequencing platforms. The AxyPrep™ FragmentSelect-I kit is optimised for Illumina next generation sequencing platforms and Life Technologies (SOLiD™) fragment size selection needs simplifying the library construction workflow.

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TriLink® CleanAmp® 7-deaza-dGTP Mix

TriLink® CleanAmp® 7-deaza-dGTP Mix

Supplier: TriLink BioTechnologies

CleanAmp® dNTPs are chemically-modified nucleotides designed to improve specificity, sensitivity, and robustness of your PCR.

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TriLink® CleanAmp® dNTP Mix

TriLink® CleanAmp® dNTP Mix

Supplier: TriLink BioTechnologies

CleanAmp® dNTPs are chemically-modified nucleotides designed to improve specificity, sensitivity, and robustness of your PCR.

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TriLink® 7-Deaza-7-Propargylamino-2'-deoxyadenosine-5'-Triphosphate

TriLink® 7-Deaza-7-Propargylamino-2'-deoxyadenosine-5'-Triphosphate

Supplier: TriLink BioTechnologies

TriLink® 7-Deaza-7-Propargylamino-2'-deoxyadenosine-5'-Triphosphate

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TriLink® 2'-Deoxyadenosine-5'-Triphosphate

TriLink® 2'-Deoxyadenosine-5'-Triphosphate

Supplier: TriLink BioTechnologies

This nucleotide consists of an unmodified base, a deoxyribose, and a 5′ triphosphate group for synthesis of DNA molecules.

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Cyanine 5-dUTP

Supplier: ENZO LIFE SCIENCES

Cyanine 5-dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Cyanine 5-dUTP can also be used for multicolor fluorescence labeling.

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Cyanine 3-UTP (enhanced)

Cyanine 3-UTP (enhanced)

Supplier: ENZO LIFE SCIENCES

Cyanine 3-UTP (Enhanced) can replace UTP as a substrate for T7 RNA polymerase in labeling systems that generate labeled probes through in vitro transcription. Probes generated by these methods are suitable for multicolor fluorescence analysis, specifically dual-color expression arrays in conjunction with cyanine 3-UTP .

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TriLink® 5-Methylcytidine-5'-Triphosphate

TriLink® 5-Methylcytidine-5'-Triphosphate

Supplier: TriLink BioTechnologies

This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.

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TriLink® Uridine-5'-O-(1-Thiotriphosphate)

TriLink® Uridine-5'-O-(1-Thiotriphosphate)

Supplier: TriLink BioTechnologies

TriLink® Uridine-5'-O-(1-Thiotriphosphate)

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TriLink® Biotin-7-Deaza-7-Propargyl-dGTP

TriLink® Biotin-7-Deaza-7-Propargyl-dGTP

Supplier: TriLink BioTechnologies

TriLink® Biotin-7-Deaza-7-Propargyl-dGTP

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TriLink® 2'-O-Methyluridine-5'-Triphosphate

TriLink® 2'-O-Methyluridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

TriLink® 2'-O-Methyluridine-5'-Triphosphate

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TriLink® 2'-Deoxycytidine-5'-Triphosphate

TriLink® 2'-Deoxycytidine-5'-Triphosphate

Supplier: TriLink BioTechnologies

This nucleotide consists of an unmodified base, a deoxyribose, and a 5′ triphosphate group for synthesis of DNA molecules.

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TriLink® 2'-Deoxyguanosine-5'-Triphosphate

TriLink® 2'-Deoxyguanosine-5'-Triphosphate

Supplier: TriLink BioTechnologies

This nucleotide consists of an unmodified base, a deoxyribose, and a 5′ triphosphate group for synthesis of DNA molecules.

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TriLink® Biotin-16-Aminoallyluridine-5'-Triphosphate

TriLink® Biotin-16-Aminoallyluridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

Biotin-16-Aminoallyluridine-5'-Triphosphate (Biotin-16-UTP, Biotin-16-AA-UTP) can be incorporated via a number of RNA polymerases, including SP6-, T3- and T7-RNA polymerase to form labeled RNA.

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5-Tetramethylrhodamine-dUTP, 1 mM Solution

Supplier: Biotium

5-TAMRA-dUTP (5-Tetramethylrhodamine-dUTP) can be enzymatically incorporated into DNA to make fluorescently labeled probes, and has absorption/emission at 553/577 nm. Note: 5-TAMRA-dUTP may not be compatible with PCR labeling of DNA probes. Supplied as a 1 mM solution in Tris-HCl buffer, pH 7.5.

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AMPIGENE® dNTP Mix

Supplier: ENZO LIFE SCIENCES

Ultra-pure, stable, and suitable for a variety of PCR applications.

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Fluorescent nucleotides, Red 594 dUTP and Red 598 dUTP

Supplier: ENZO LIFE SCIENCES

Red dUTP's (Red 594 dUTP, Red 598 dUTP) can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.

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Dideoxyuridine triphosphates (ddUTPs)

Supplier: ENZO LIFE SCIENCES

These ddUTPs are used for 3’-end labeling of oligodeoxynucleotides using terminal transferase. ddUTP also serves as a substrate for E. coli DNA Polymerase I (holoenzyme and Klenow fragment), T4 and T7 DNA polymerases, Taq DNA polymerase and reverse transcriptase.

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Cyanine 3-dUTP

Cyanine 3-dUTP

Supplier: ENZO LIFE SCIENCES

Cyanine 3-dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Cyanine 3-dUTP can also be used for multicolor fluorescence labeling.

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TriLink® 5-Methoxyuridine-5'-Triphosphate

TriLink® 5-Methoxyuridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.

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TriLink® Adenosine-5'-Triphosphate

TriLink® Adenosine-5'-Triphosphate

Supplier: TriLink BioTechnologies

This nucleotide consists of an unmodified base, a ribose, and a 5′ triphosphate group for synthesis of RNA molecules.

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TriLink® Cytidine-5'-Triphosphate

TriLink® Cytidine-5'-Triphosphate

Supplier: TriLink BioTechnologies

This nucleotide consists of an unmodified base, a ribose, and a 5′ triphosphate group for synthesis of RNA molecules.

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PPM1B Human siRNA Oligo Duplex (Locus ID 5495)

Supplier: OriGene

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways.

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