Činidla pro nukleové kyseliny

Supplier: OMEGA BIO-TEK

Souprava E.Z.N.A.® bacterial DNA kit umožňuje rychlou a spolehlivou izolaci vysoce kvalitní celkové buněčné DNA ze širokého spektra bakterií. Souprava využívá optimálních lyzačních podmínek, v každé kolonce lze zpracovat až 1×10⁹ bakteriálních buněk. Bez organické extrakce - úspora času i plastového odpadu, což umožňuje zpracovávat současně více vzorků. Bakteriální buňky jsou pěstovány do log-fáze a následně použity. Buněčná stěna je odstraněna štěpením lysozymem a mechanicky pomocí kuliček, následně štěpením proteázou. Po lyzi jsou upraveny vazebné podmínky a vzorek je dále purifikován pomocí HiBind DNA centrifugačních kolonek. Dva rychlé promývací kroky odstraní stopy solí a kontaminujících proteinů a poté je DNA eluována vodou nebo elučním pufrem. Purifikovaná DNA může být přímo použita pro následné aplikace bez nutnosti dalšího čištění.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Supplier: Quantabio

sparQ Universal Library Quant Kit provides rapid and accurate quantification of libraries prepared for sequencing on Illumina® NGS platforms.

Supplier: OMEGA BIO-TEK

Purifikace DNA z gelů je běžná technika používaná pro izolaci specifických fragmentů DNA. Většina metod však buďto neumožňuje kompletní odstranění agarózy (což může vést k problémům při následných aplikacích), nastříhá řetězce DNA nebo poskytuje velmi nízké výtěžky.

Supplier: OMEGA BIO-TEK

Plazmid izolovaný pomocí tradičních purifikačních metod běžně obsahuje vysoké hladiny endotoxinů, které mohou významně interferovat s následnými transfekčními experimenty. U souprav E.Z.N.A.® Endo-Free Plasmid Kit je do postupu plazmidové purifikace za účelem získání plazmidu o vysoké kvalitě vhodného pro transfekci včleněn krok efektivního odstranění endotoxinů.

Supplier: OMEGA BIO-TEK

RNA-Solv® Reagent is a one reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is a modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenised and lysed in RNA-Solv® Reagent, which maintains the integrity of the RNA while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.

Supplier: Thermo Fisher Scientific

AATTC sites and cuts best at 37 °C in its own unique buffer. Exhibit 100% activity in the recommended buffer and reaction conditions.

Supplier: Quantabio

AccuStart™ Genotyping ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes.

Supplier: Apollo Scientific

Recombinant Taq DNA polymerase produced in E.coli 20 U/µl.

Supplier: TriLink BioTechnologies

2',3'-Dideoxycytidine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddCTP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.

Supplier: Corning

The efficient removal of unincorporated dNTPs, salts and enzymes after any PCR is detrimental to the success or failure of downstream applications, such as genotyping, Sanger, and Next Generation Sequencing. The AxyPrep™ Mag PCR Clean Up Kit utilises a paramagnetic bead technology for rapid high throughput purification of PCR products. Additionally, the AxyPrep™ Mag PCR Clean Up Kit employs an optimised buffer to selectively bind PCR amplicons 60 bp and larger to paramagnetic beads, providing end users flexibility and high recovery options. The protocol comprises binding, washing and eluting steps, where primers, nucleotides, salts and enzymes in the reaction mixture are efficiently removed leaving a purified PCR product that is essentially free of contaminants. No centrifugation or filtration steps are required, allowing the process to be adapted to multiple commercially available automation platforms.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Food DNA Kit allows rapid and reliable isolation of high quality DNA from complex matrixes such as processed food, chocolate, cereals and meat.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Blood RNA Kit is designed for the isolation of total intracellular RNA from up to 1 ml of fresh, or frozen whole blood treated with any common anticoagulant such as heparin, EDTA or acid-citrate-dextrose. The procedure completely removes contaminants and enzyme inhibitors making total RNA isolation fast, convenient and reliable. Red blood cells are selectively lysed and white cells are collected by centrifugation. After lysis of white blood cells under denaturing conditions that inactivate RNases, the lysate is homogenised with a homogeniser spin column. The sample is then applied to a HiBind® spin column. Cellular debris and other contaminants such as haemoglobin are effectively washed away and high-quality RNA is finally eluted in DEPC treated water.

Supplier: TriLink BioTechnologies

TriLink® 2'-Deoxyguanosine-5'-O-(1-Thiotriphosphate)

Supplier: Abcam

ab185908 is a complete set of reagents required for carrying out a successful ChIP-Seq starting from mammalian cells or tissues.

Supplier: Abcam

Genomic DNA Extraction Kit (ab156900) is designed for rapid isolation of pure genomic DNA from blood leukocytes or cultured mammalian cells in a small amount.