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Systémy pro purifikaci proteinů

Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.

Sepharose™ CL-2B Gel Filtration Base Matrix

Sepharose™ CL-2B Gel Filtration Base Matrix

Supplier: Cytiva

Sepharose CL-2B is a well-proven cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

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Affinity chromatography media, Ni Sepharose™ 6 Fast Flow

Affinity chromatography media, Ni Sepharose™ 6 Fast Flow

Supplier: Cytiva

Ni Sepharose 6 Fast Flow is a BioProcess medium that combines the advantages of Ni₂₊ for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.

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CM Sephadex™ C-25 Ion Exchange Chromatography Media

CM Sephadex™ C-25 Ion Exchange Chromatography Media

Supplier: Cytiva

Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.

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Con A Sepharose™ 4B Affinity Chromatography Media, Cytiva

Supplier: Cytiva

Con A Sepharose is Concanavalin A coupled to Sepharose 4B by the cyanogen bromide method.

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Ion exchange chromatography media, DEAE Sephacel™

Ion exchange chromatography media, DEAE Sephacel™

Supplier: Cytiva

DEAE Sephacel™ is a weak anion exchanger based on beaded cellulose. The ion exchange group is diethylaminoethyl, which remains charged and maintains consistently high capacity over the entire working range. This media is macroporous and has an exclusion limit of ~10000 kDa for globular proteins. Supplied pre-swollen in 20% ethanol.

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Affinity coupling media, Epoxy-Activated Sepharose™ 6B

Supplier: Cytiva

Epoxy-activated Sepharose™ 6B is a pre-activated medium for immobilisation of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose™ 6B via a 12-atom hydrophilic spacer arm. Medium is formed by reacting Sepharose™ 6B with 1,4-bis (2,3- epoxy-propoxy-) butane. It can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups.

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Sepharose™ CL-6B Gel Filtration Base Matrix

Sepharose™ CL-6B Gel Filtration Base Matrix

Supplier: Cytiva

Sepharose™ CL-6B is a cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix.

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Affinity chromatography media, MabSelect SuRe™

Affinity chromatography media, MabSelect SuRe™

Supplier: Cytiva

MabSelect SuRe™ is composed of a rigid, high-flow agarose matrix and alkali-tolerant rProtein A ligand. The ligand has been engineered to provide greater stability than conventional protein A-based media in the alkaline conditions used in CIP protocols. This enhanced alkali stability improves process economy and product quality.

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Plasmid purification media, PlasmidSelect Xtra

Plasmid purification media, PlasmidSelect Xtra

Supplier: Cytiva

PlasmidSelect Xtra is a thiophilic aromatic adsorption chromatography medium with a selectivity that allows generic purification of supercoiled covalently closed circular forms of plasmid DNA to be separated from open circular forms.

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Gel filtration media, Superdex™ 30 PG

Gel filtration media, Superdex™ 30 PG

Supplier: Cytiva

Superdex prep grade is a preparative gel filtration medium with a composite matrix of dextran and agarose

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Hydrophobic interaction chromatography media, Butyl Sepharose™ 4 Fast Flow

Hydrophobic interaction chromatography media, Butyl Sepharose™ 4 Fast Flow

Supplier: Cytiva

Butyl Sepharose 4 Fast Flow is part of the Sepharose Fast Flow HIC platform, which has been an industrial standard for HIC chromatography during recent decades.

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Affinity chromatography media, MabSelect™

Affinity chromatography media, MabSelect™

Supplier: Cytiva

MabSelect™ is a protein A affinity resin designed for capturing monoclonal antibodies (mAbs) from large sample volumes.

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Affinity chromatography media, Benzamidine Sepharose™ 4 Fast Flow (High Sub)

Affinity chromatography media, Benzamidine Sepharose™ 4 Fast Flow (High Sub)

Supplier: Cytiva

Benzamidine Sepharose™ 4 Fast Flow (High Sub) is a tool for removal and/or purification of trypsin and trypsin-like serine proteases, as well as zymogens, including urokinase and prekallikrein.

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Accessories for ÄKTA pure

Supplier: Cytiva

Tubing kit I. Ø. 0,25 mm, for ÄKTA pure 25

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Gel filtration media, Sephadex™ LH-20

Gel filtration media, Sephadex™ LH-20

Supplier: Cytiva

Sephadex™ LH-20 is a liquid chromatography media for molecular sizing of natural product such as steroid, terpenoid, lipid and low molecular weight peptides. Sephadex™ LH-20 is pre-pared by hydroxypropylation of Sephadex™ G-25, a bead-formed dextran medium.

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Affinity chromatography media, Streptavidin Sepharose™ High Performance

Affinity chromatography media, Streptavidin Sepharose™ High Performance

Supplier: Cytiva

Streptavidin Sepharose™ High Performance is an affinity chromatography medium designed for fast and reliable, high resolution purification of biotinylated biomolecules. Purified Streptavidin isolated from Streptomyces avidinii is immobilised on Sepharose™ High Performance.

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Multimodal chromatography media, Capto™ MMC

Multimodal chromatography media, Capto™ MMC

Supplier: Cytiva

Capto™ MMC is a multimodal cation exchanger with a different selectivity, compared with traditional ion exchangers.

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Affinity chromatography media, TALON® Superflow™

Affinity chromatography media, TALON® Superflow™

Supplier: Cytiva

Cobalt-based chromatography medium designed for purification of histidine-tagged recombinant proteins by immobilised metal affinity chromatography (IMAC).

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Affinity chromatography media, MabSelect Xtra™

Affinity chromatography media, MabSelect Xtra™

Supplier: Cytiva

MabSelect Xtra has the same recombinant Protein A ligand as MabSelect but is based on a high-flow agarose base matrix with increased porosity and a slightly decreased particle size compared to MabSelect.

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Accessories for chromatography systems

Accessories for chromatography systems

Supplier: Cytiva

Tubing/Pump P-3, with stop collars, 0,14 m, silicone

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Gelatin-Sepharose® 4B

Gelatin-Sepharose® 4B

Supplier: Cytiva

Gelatin Sepharose® 4B affinity media with immobilised gelatin is designed for the purification or depletion of fibronectin as gelatin binds to fibronectin with high specificity. Gelatin Sepharose® 4B is bovine gelatin coupled to Sepharose® 4B by the cyanogen bromide method.

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Capto™ adhere ImpRes multimodal chromatography media, Cytiva

Capto™ adhere ImpRes multimodal chromatography media, Cytiva

Supplier: Cytiva

Capto™ adhere ImpRes is a strong anion exchange multimodal BioProcess chromatography medium (resin), designed to meet the demands of modern large-scale manufacturers, for high-resolution polishing of monoclonal antibodies (MAbs) and other biomolecules. Capto™ adhere ImpRes is designed to allow effective MAb polishing in the second or third step of a purification scheme downstream of the protein A capture step. Polishing can be performed in either bind and elute (binding) or flowthrough (non binding) modes.

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Gel filtration calibration kits

Gel filtration calibration kits

Supplier: Cytiva

Gel Filtration Calibration Kits are suitable for calibration of high-resolution gel filtration media and columns, such as Superdex, Superose and Sephacryl.

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Affinity chromatography media, Dextrin Sepharose™ High Performance

Affinity chromatography media, Dextrin Sepharose™ High Performance

Supplier: Cytiva

Dextrin Sepharose™ High Performance is an affinity media for purification of recombinant proteins tagged with Maltose Binding Protein (MBP).

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Gel filtration media, Sephadex™ G-10

Gel filtration media, Sephadex™ G-10

Supplier: Cytiva

Sephadex™ G-10 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >700 molecular weight.

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Sepharose™ CL-4B Gel Filtration Base Matrix

Sepharose™ CL-4B Gel Filtration Base Matrix

Supplier: Cytiva

Sepharose™ CL-4B is a cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix.

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Affinity chromatography media, Chelating Sepharose™ Fast Flow

Affinity chromatography media, Chelating Sepharose™ Fast Flow

Supplier: Cytiva

Chelating Sepharose Fast Flow is composed of cross-linked 6% agarose beads modified with iminodiacetic immobilized to the base matrix by stable ether linkages and sufficiently long spacer arms.

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Multimodal chromatography media, Capto™ Core 700

Multimodal chromatography media, Capto™ Core 700

Supplier: Cytiva

Capto™ Core 700 is designed for intermediate purification and polishing of viruses and other large biomolecules (molecular weight >700000).

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2D Clean-Up kit

2D Clean-Up kit

Supplier: Cytiva

The 2-D Clean-Up Kit is designed to prepare samples for 2-D electrophoresis that otherwise produce poor 2-D spot-maps due to high conductivity, high levels of interfering substances, or low protein concentration. The reagents quantitatively precipitate proteins while leaving interfering substances, such as detergents, salts, lipids, phenolics, and nucleic acids, in solution.

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Protein purification system, ÄKTA™ start

Protein purification system, ÄKTA™ start

Supplier: Cytiva

ÄKTA start is an easy to use chromatography system that automates manual purification procedures using, for example, HiTrap columns. It offers an entry level chromatography solution for the preparative purification of proteins at lab scale.

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