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329 výsledky pro Systémy pro purifikaci proteinů

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Systémy pro purifikaci proteinů

Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.

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Immunoprecipitation, magnetic IP/Co-IP kit, c-Myc-tag, Pierce™

Immunoprecipitation, magnetic IP/Co-IP kit, c-Myc-tag, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ c-Myc-Tag Magnetic IP/Co-IP Kit contains specific immunoaffinity magnetic beads and reagents to perform immunoprecipitation assays of c-Myc fusion proteins or co-IP experiments using c-Myc-tagged bait proteins.

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HA-Tag Magnetic IP/Co-IP Kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ HA-Tag Magnetic IP/Co-IP Kit contains specific immunoaffinity magnetic beads and reagents to perform immunoprecipitation assays of HA fusion proteins or co-IP experiments using HA-tagged bait proteins.

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Affinity purification media, glutathione agarose, Pierce™

Affinity purification media, glutathione agarose, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Glutathione Agarose is a high-capacity, high-performance resin for affinity purification of GST-tagged fusion proteins from cellular lysates.

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Immobilised ficin (agarose resin), Pierce™

Immobilised ficin (agarose resin), Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Immobilised Ficin consists of a sulfhydryl-specific protease (ficin) that has been immobilised onto beaded agarose resin to enable controlled antibody fragmentation, especially of mouse IgG1.

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Monomeric avidin resin, UltraLink™, Pierce™

Monomeric avidin resin, UltraLink™, Pierce™

Supplier: Thermo Fisher Scientific

UltraLink™ Monomeric Avidin resin is ideal for affinity purification of biotinylated proteins, peptides and other molecules. Immobilisation of avidin monomers results in a purification resin with a much lower biotin-binding affinity than native tetrameric avidin, enabling recovery of biotinylated molecules using mild elution conditions.

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Recombinant protein L, Pierce™

Recombinant protein L, Pierce™

Supplier: Thermo Fisher Scientific

Purified (unconjugated) Pierce™ Recombinant Protein L is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of mouse and human antibodies in immunoassays and antibody purification protocols.

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Pierce™ Pegylation Reagents, Carboxy-PEG-Amine Compounds

Pierce™ Pegylation Reagents, Carboxy-PEG-Amine Compounds

Supplier: Thermo Fisher Scientific

The carboxy-PEGn-amine (CA[PEG]n) PEGylation reagents are zwitterionic, amino acid derivatives that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers. These PEGylation reagents are homogenous compounds of defined molecular weight and spacer length, providing precision in optimising modification applications.

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Pierce™ Conjugate Purification Kit

Pierce™ Conjugate Purification Kit

Supplier: Thermo Fisher Scientific

Pierce™ conjugate purification kit is designed to eliminate excess, unconjugated HRP or alkaline phosphatase enzyme from labeled antibodies (IgG) after performing antibody-enzyme conjugation reactions. The single-use column-kit is intended for purification of 0,5 to 50 mg of antibody conjugate dissolved in tris-buffered saline.

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Glutathione magnetic agarose, Pierce™

Glutathione magnetic agarose, Pierce™

Supplier: Thermo Fisher Scientific

These beads provide a fast, convenient method for purification of glutathione-S-transferase (GST) from a bacterial, yeast, or mammalian crude cell lysate.

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Direct IP kit, Pierce™

Direct IP kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Direct IP Kit uses an activated resin to covalently immobilise IP antibodies (any species or class) on agarose beads without the aid of Protein A/G, enabling immunoprecipitation without antibody interference.

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Spin purification kit for GST proteins, Pierce™

Spin purification kit for GST proteins, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ GST Spin Purification Kit provides a fast, easy to use format for purifying GST-fusion proteins from cellular lysates.

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Coupling catalyst, GlycoLink™

Coupling catalyst, GlycoLink™

Supplier: Thermo Fisher Scientific

An optimized buffer for aldehyde-hydrazide and aldehyde-alkoxyamine coupling reactions.

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Pegylation reagents, methyl-PEG-amine compounds

Pegylation reagents, methyl-PEG-amine compounds

Supplier: Thermo Fisher Scientific

The methyl-PEGn-amine (MA[PEG]n) PEGylation reagents are methyl ether-terminated PEG amines that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers. These PEGylation reagents are homogenous compounds of defined molecular weight and spacer length, providing precision in optimising modification applications.

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Co-immunoprecipitation (Co-IP) Kit, Pierce™

Co-immunoprecipitation (Co-IP) Kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Co-Immunoprecipitation (Co-IP) Kit provides covalent antibody immobilisation and all the components necessary to perform a properly controlled co-IP experiment without antibody interference in the final product.

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Mass spectrometry reagents, MALDI matrices, single-use

Mass spectrometry reagents, MALDI matrices, single-use

Supplier: Thermo Fisher Scientific

MALDI Matrices are highly purified, recrystallised reagents packaged in convenient single-use tubes sufficient for analysing up to 96 samples.

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Pierce™ Agarose ChIP Kit

Pierce™ Agarose ChIP Kit

Supplier: Thermo Fisher Scientific

Provides a complete set of reagents and a simple, fast and reproducible protocol to perform chromatin immunoprecipitation (ChIP) assays.

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Pegylation reagent, MT(PEG)₄ methyl-PEG-thiol compound

Pegylation reagent, MT(PEG)₄ methyl-PEG-thiol compound

Supplier: Thermo Fisher Scientific

Methyl-PEG4-thiol is a methyl- and sulfhydryl-terminated compound that contains a 4-unit polyethylene glycol (PEG) spacer and is used to modify surfaces such as quantum dots, monolayers and magnetic particles.

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Agarose coupling kits, HOOK™

Agarose coupling kits, HOOK™

Supplier: G-Biosciences

The amine reactive HOOK™ Agarose Coupling Kit is designed for the simple and efficient coupling of proteins and peptides to a solid support. The kit allows the generation of both a stable and reusable column for the affinity purification of antibodies, proteins and other protein interacting biomolecules.

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Sulphhydryl immobilisation kit for proteins and peptides

Sulphhydryl immobilisation kit for proteins and peptides

Supplier: G-Biosciences

This Sulphhydryl Immobilisation Kit is designed for the coupling of sulphhydryl containing peptides to a solid agarose support. The sulphhydryl coupling resin columns utilises iodoacetyl groups that specifically react with free sulphhydryls to form covalent, permanent thioether bonds.

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Immobilised Catalase

Supplier: G-Biosciences

Immobilised Catalase is prepared by covalent coupling of catalase enzyme to 6% cross-linked agarose beads. Immobilised Catalase is used to remove hydrogen peroxide from biological samples for use in downstream applications which require absenceof hydrogen peroxide as it causes interference.

 

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Pierce™ F(ab')2 Preparation Kit

Supplier: Thermo Fisher Scientific

Pierce F(ab')2 preparation kit uses immobilised pepsin protease to selectively digest whole human and other IgG antibodies to make F(ab')2 fragments that retain antigen binding activity.

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HCCA matrix

Supplier: BRUKER DALTRONIK

Pre-portioned HCCA matrix provides easy and convenient preparation of HCCA matrix solutions.

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Control agarose resin, Pierce™

Control agarose resin, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Control Agarose Resin is underivatised beaded agarose support for pre-clearing protein immunoprecipitation samples.

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BTSNM support materials, Chelex beads, 0,4 g with buffer

Supplier: G-Biosciences

Chelex beads are made of a styrene divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions.

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Recombinant streptavidin

Recombinant streptavidin

Supplier: G-Biosciences

Recombinant streptavidin from Streptomyces avidinii, produced in E.coli.

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Immobilised β-cyclodextrin

Supplier: G-Biosciences

Immobilised β-cyclodextrin is designed to allow refolding of recombinant proteins that were aggregated or formed inclusion bodies when expressed in the genetically engineered cells. The refolding of misfolded proteins is adapted from GroE chaperone refolding mechanism.

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Pegylation reagent, CT(PEG)₁₂ carboxy-PEG-thiol compound

Pegylation reagent, CT(PEG)₁₂ carboxy-PEG-thiol compound

Supplier: Thermo Fisher Scientific

Carboxy-PEG12-thiol, is a carboxyl- and sulphhydryl-terminated compound that contains a 12-unit polyethylene glycol (PEG) spacer and is used to modify surfaces such as quantum dots, monolayers and magnetic particles.

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Protein G Sepharose™ 4 Fast Flow Affinity Chromatography Media

Protein G Sepharose™ 4 Fast Flow Affinity Chromatography Media

Supplier: Cytiva

Protein G Sepharose 4 Fast Flow has recombinant protein G immobilized by the cyanogen bromide (CNBr) method to Sepharose 4 Fast Flow.

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Affinity coupling media, CNBr-Activated Sepharose™ 4B

Affinity coupling media, CNBr-Activated Sepharose™ 4B

Supplier: Cytiva

CNBr-activated Sepharose™ 4B is pre-activated media used for coupling antibodies or other large proteins containing -NH₂ groups to the Sepharose™ media, by the cyanogen bromide method, without an intermediate spacer arm.

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Affinity coupling media, NHS-Activated Sepharose™ 4 Fast Flow

Affinity coupling media, NHS-Activated Sepharose™ 4 Fast Flow

Supplier: Cytiva

NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. The resin provides a spacer arm to the coupled ligand and suitable for immobilising smaller protein and peptide ligands.

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