Hledali jste: Systémy pro purifikaci proteinů

Supplier: Abcam

Sample Diluent NBP is part of the reagents, controls and accessories range.

Supplier: Genscript

Anti-His affinity resins are designed for purification of His-tagged protein. Composed of agarose and conjugated THE™ anti-His monoclonal antibody, they are compatible with most commonly used reagents at certain concentrations.

Supplier: G-Biosciences

The Pearl™ Antibody Clean Up Kit is designed for the rapid clean up of antibody solutions using a combination of our Pearl™ IgG Purification Resin to remove the protein stabilisers and SpinOUT™ desalting columns to ensure the antibody solutions are in an optimal buffer for clean up. It binds high abundant, non IgG proteins (i.e. BSA and gelatin) and allows the IgG molecules to pass through in a physiological buffer. For the purification of ten 0,5 ml IgG samples with up to 1% BSA and gelatin.

Supplier: G-Biosciences

The Pearl™ Monoclonal IgG Purification Kit allows for the rapid purification of antibodies from cell culture supernatant and ascites fluid. The Pearl™ IgG purification resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer. The IgG molecules can be stored or used in downstream applications without further clean-up, such as ammonium sulphate precipitation.

Supplier: Abcam

Protein A Agarose (ab193254) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

Supplier: G-Biosciences

The SpinOUT™ GT-100, GT-600 and GT-1200 columns are versatile, spin format columns for the desalting and buffer exchange of protein solutions ranging from 5 µl through to 4 ml sample volumes. The SpinOUT™ columns are available in three MWCO sizes for >700, >6000 or >30 000 dalton proteins and are suitable for samples containing as little as 20 µg protein/ml.

Supplier: Rockland Immunochemicals

Protein A Sepharose™ is a suspension of beads conjugated to native Protein A derived from Staphylococcus aureaus. Protein A has a high affinity for the Fc regions of IgG molecules from a variety of species, including human, mouse, rat IgG2c, cow IgG2c, goat IgG2, sheep IgG2 and rabbit.

Supplier: G-Biosciences

Following digestion of proteins with proteases, the addition of Pep-Extract™ rapidly elutes digested peptides in under 20 minutes. The eluted peptides can be used directly in mass spectrometry analysis without any additional clean up. Contains sufficient for 500 protein spots.

Supplier: Abcam

Streptavidin-HRP Solution (ab210901) is part of the reagents, controls and accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

Supplier: Abcam

His-Tag Protein Purification Column (Pre-packed, 5 x 1ml) - AminTrap (ab270528) is a simple and easy ready to use chromatography medium for purifying His-tagged proteins that have been expressed in series of expression vectors, such as E.coli., yeast, insect cells and mammalian cells.

Supplier: Abcam

ATP-Agarose (ab270535) comprises ATP attached to agarose beads.

Supplier: Abcam

Abcam's BSA Removal Kit( ab173231) offers: Efficiency: It provides a simple one-step, 10-minute method to effectively separate BSA from antibodies. Versatility: The kit can be used on any antibody subtype and species, making it highly adaptable for various research needs. Convenience: The antibody is left in a suitable position for transfer to a buffer more suited to conjugation, streamlining the preparation process.

Supplier: ImmunoReagents

ImmunoReagents Donkey IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Donkey IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

Supplier: G-Biosciences

The Pearl™ IgG purification kits are designed for the one step purification of IgG antibodies from serum. The supplied Pearl™ IgG purification resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer. The purified IgG molecules can be stored or used in further downstream applications without further clean-up, such as ammonium sulphate precipitation.

Supplier: ImmunoReagents

ImmunoReagents Bovine IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Bovine IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

Supplier: Abcam

Antibodies are sometimes only available at concentrations that are too low for antibody conjugation or other antibody applications.

Supplier: Abcam

His-Tag Protein Purification Column (Pre-packed, 5 x 5ml) - AminTrap (ab270529) is a simple and easy ready to use chromatography medium for purifying His-tagged proteins that have been expressed in series of expression vectors, such as E.coli., yeast, insect cells and mammalian cells.

Supplier: Abcam

TCS Antibody Purification Kit - Nanoparticles (ab270554) is designed for the purification of IgG fractions from tissue culture supernatants.

Supplier: Abcam

Anti-HA Affinity Resin - Amintra (ab270603) is designed for simple, rapid HA-tagged recombinant protein purification and immunoprecipitation.

Supplier: Abcam

Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. ab128745 quickly removes these contaminants.

Supplier: G-Biosciences

Cobalt chelating resin, for the purification of 6X His proteins

Supplier: ImmunoReagents

ImmunoReagents Mouse IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Mouse IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

Supplier: G-Biosciences

Glutathione resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 4% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of ~8 mg GST/ml resin. Supplied as slurry in 20% ethanol.

Supplier: G-Biosciences

Thiophilic adsorption or thiophilic chromatography is a routinely used technique for the low cost, simple purification of immunoglobulins. Thiophilic adsorption was first developed by Porath et al in 1984 and is a group specific, salt-dependent purification technique that has distinct affinity towards immunoglobulins and α2- macroglobulins. The thiophilic adsorption works on the principle that some proteins in high salt are able to bind to an immobilised ligand that contains a sulfone group in proximity to a thioether group. The bound proteins are then eluted in decreasing salt concentrations. The thiophilic resin binds immunoglobulins, including IgG, IgY and IgM, from serum, ascites or tissue culture supernatants and the purified immunoglobulins are then eluted in a near neutral aqueous buffer.

Supplier: Abcam

TMB substrate recommended for use with the Matched Antibody Pair kits for sandwich ELISA.

Supplier: G-Biosciences

This avidin resin is designed for the single step small and large scale affinity purification of proteins and antibodies with a biotin tag. The resin can also be used for immunoprecipitations using biotin labelled antibodies.

Supplier: Biotium

TEV protease kit that may be used for cleaving proteins or peptides containing the TEV protease recognition sequence E-N-L-Y-F-Q↓(S/G/A/M/C/H).

Supplier: Abcam

TCS Antibody Purification Kit (ab109207) has been designed for tissue culture supernatant purification, and can be used to purify 10-50 mL in each purification.

Supplier: Abcam

Diluent M Concentrate (10X) (ab138908) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

Supplier: G-Biosciences

This sulphhydryl coupling resin is designed for coupling of peptides and proteins to a solid 6% agarose support through free sulfhydryl groups (-SH). The iodoacetyl groups of the sulfhydryl coupling resin specifically react with free sulfhydryls to form covalent, permanent thioether bonds.