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Systémy pro purifikaci proteinů

Systémy pro purifikaci proteinů

Systémy pro purifikaci proteinů jsou určeny k izolaci jednoho nebo více proteinů z komplexní směsi. Pracují s buňkami, tkáněmi nebo celými organismy a čistí podle předdefinovaných počátečních bodů a upravitelných metodických šablon. Jediným kliknutím nabízí kompaktní zařízení kontrolu a vizualizaci běhu v reálném čase. Jsou vhodné i pro práci v chladírenských podmínkách a umožňují purifikaci afinitně značených proteinů, protilátek, neoznačených či native proteinů a také čištění vzorků.

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J.T.Baker® BAKERBOND® PROchievA™, Affinity Chromatography Resin, Recombinant Protein A

J.T.Baker® BAKERBOND® PROchievA™, Affinity Chromatography Resin, Recombinant Protein A

Supplier: Avantor

The J.T.Baker® BAKERBOND® PROchievA™ protein A resin offers superior dynamic binding capacity with excellent alkaline stability linked to Avantor's proprietary ligand.

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Destičky pro kapalinovou extrakci, J.T.Baker® Bakerbond®

Destičky pro kapalinovou extrakci, J.T.Baker® Bakerbond®

Supplier: Avantor

Destičky J.T.Baker® BAKERBOND® pro kapalinovou extrakci (SLE) jsou ideální a rychlejší alternativou k tradičním metodám extrakce kapalina-kapalina a umožňují vynikající výtěžky analytů z biologických matric.

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HRP Conjugate Stabilizer - LifeXtend, Abcam

Supplier: Abcam

HRP Conjugate Stabilizer - LifeXtend (ab270548) is a proprietary multi-component reagent system that protects antibody–HRP conjugates from instability, microbrial attack, and denaturation, thus ensuring the best possible performance in experiments performed at room temperature.

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GTP-Agarose, Abcam

Supplier: Abcam

GTP-Agarose (ab270533) comprises GTP attached to agarose beads.

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Affinity purification Streptavidin Resin and buffers

Supplier: G-Biosciences

Streptavidin Resin is designed for the single step small and large scale affinity purification of proteins and antibodies with a biotin tag. Biotin, a 244 Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10¹⁵ M⁻¹) and streptavidin (Ka=10¹⁵ M⁻¹). Biotin and (strept)avidin interaction is rapid and once the bond is established it can survive up to 3M guanidinehydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at pH 1,5 or by autoclaving. The biotinylated molecules are efficiently probed with avidin or streptavidin conjugated to reporter molecules, such as peroxidases or phosphatases.

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Immunoprecipitation kit, Abcam

Supplier: Abcam

Abcam's Immunoprecipitation Kit (ab206996) can be used to perform immunoprecipitation (IP) and Co-IP for functional studies of immunoprecipitated proteins/complexes and SDS-PAGE or western blot analysis of immunoprecipitated proteins and complexes.

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BSA Removal Kit (Gold/Europium labelling), Abcam

Supplier: Abcam

Our BSA Removal Kit - Nanoparticles (ab204912) is a simple one-step, 10 minute method that effectively separates BSA from the antibody.

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Mouse TCS Antibody Purification Kit, Abcam

Supplier: Abcam

Efficient mouse antibody purification from tissue culture supernatants.

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Destičky k precipitaci proteinu J.T.Baker® Bakerbond®

Destičky k precipitaci proteinu J.T.Baker® Bakerbond®

Supplier: Avantor

Destičky k precipitaci proteinu J.T.Baker® Bakerbond® umožňují rychlé odstranění proteinů z biologických vzorků.

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Antibody Purification Kit (Gold/Europium labelling), Abcam

Supplier: Abcam

Antibody Purification Kit - Nanoparticles (ab204909) removes common buffer constituents found in commercial antibodies.

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Human affinity purification resin

Human affinity purification resin

Supplier: ImmunoReagents

ImmunoReagents Human IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Human IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

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Protein G

Protein G

Supplier: Rockland Immunochemicals

Protein G is a surface protein of two groups of streptococcal bacteria that has ability to bind immunoglobulins and also to albumins. Protein G are prepared from chromatographically pure protein G.

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Immobilised Pepstatin for purification of Cathepsins/Pepsin/Pepstatin binding molecules

Immobilised Pepstatin for purification of Cathepsins/Pepsin/Pepstatin binding molecules

Supplier: G-Biosciences

Immobilised Pepstatin is for the purification of cathepsins, pepsin, bacterial aspartic proteases, HIV proteases and other molecules that bind pepstatin.

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Jacalin for IgA purification

Jacalin for IgA purification

Supplier: G-Biosciences

Jacalin, or Artocarpus integrifolia lectin, is a tetrameric two-chain lectin with a molecular weight of 66 kDa. It is a α-D-galactose binding lectin purified from jack fruit (Artocarpus integrifolia) seeds.

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Serum Antibody Purification Kit (Protein G), Abcam

Supplier: Abcam

Protein G has a high affinity for the Fc regions of IgG molecules from a variety of species.

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Llama affinity purification resin

Llama affinity purification resin

Supplier: ImmunoReagents

ImmunoReagents Llama IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Llama IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

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