Hledali jste: Membrány transferové

Supplier: Cytiva (Formerly Pall Lab)

Transferové membrány Biodyne® jsou velmi senzitivní a mají nízké pozadí pro lepší detekci a rozlišení. Jsou ideálníi pro detekci nukleových kyselin.

Supplier: Cytiva (Formerly Pall Lab)

Přirozeně hydrofobní PVDF, ideální pro široké rozpětí proteinových analýz. Skupina médií FluroTrans® je bílá mikroporózní pevná podpora, která váže proteiny hydrofobními interakcemi. Membrána FluoroTrans® je optimalizovaná pro N-terminal sekvenování proteinů. Membrána FluoroTrans® W je optimilizovaná pro transferové aplikace Western. Membrána FluoroTrans® W umožňuje citlivou detekci proteinů s nízkým pozadím a velmi nízké spálení proteinu. Imobilizované proteiny lze vizuálně detekovat pomocí Coomassie® Blue, Amido Black, Ponceau S a koloidním zlatem.

Supplier: Thermo Fisher Scientific

Nitrocellulose membranes are a popular matrix used in protein blotting because of their high protein-binding affinity, compatibility with a variety of detection methods (chemiluminescence, chromogenic, and fluorescence), and the ability to immobilise proteins, glycoproteins, or nucleic acids. Protein immobilisation is thought to occur by hydrophobic interactions, and high salt and low methanol concentrations help improve protein immobilisation to the membrane during electrophoretic transfer, especially for proteins with higher molecular weights. Nitrocellulose membranes are not optimal for electrophoretic transfer of nucleic acids, as the high salt concentrations that are required for efficient binding will effectively elute some or all of the charged nucleic acid fragments.

Supplier: Thermo Fisher Scientific

Biodyne™ A nylon membranes are sheets of unmodified nylon that provide excellent binding characteristics for nucleic acid transfer, blotting and detection methods. Biodyne™ A nylon membrane is manufactured with a matrix pore size of 0,45 micron. It contains both negatively and positively charged groups, enabling it to bind many kinds of macromolecules.

Supplier: THERMO OWL SCIENTIFIC

Hydrophobic polyvinylidene fluoride transfer membrane has a high signal-to-noise ratio, which allows for the use of mild blocking procedures

Supplier: Thermo Fisher Scientific

Transfer membranes made of high quality PVDF, which provides high binding capacity for proteins and nucleic acids.

Supplier: Thermo Fisher Scientific

Biodyne™ B nylon membranes are sheets of positively charged nylon that provide excellent binding characteristics for nucleic acid transfer, blotting and detection methods. Biodyne™ B nylon membrane is manufactured with a matrix pore size of 0,4 micron. It contains exclusively positive charges, providing the highest possible binding capacity for negatively charged molecules, like DNA and RNA.

Supplier: Whatman products (Cytiva)

Membrány Protran® jsou vyrobeny ze 100% čisté nitrocelulózy, aby byla zajištěna nejvyšší možná vazebná kapacita. Jsou kompatibilní s řadou detekčních metod, včetně izotopových, chemiluminiscenčních, kolorimetrických a fluorescenčních. Není vyžadován žádný krok předmáčení methanolem, což z této membrány dělá volbu pro proteiny, které preferují vodné prostředí. Před přenosem je membrána jednoduše navlhčena vodou a poté umístěna do přenosového pufru. Žádné další kroky předběžného ošetření nejsou nutné.

Supplier: Whatman products (Cytiva)

Nylonové membrány Nytran® SuPerCharge (SPC) mají velice vysoký kladný náboj a vyšší hustotu nylonu na jednotku plochy. Dostupné jako kruhové výseče, archy nebo role pro mikrodestičky.

Supplier: G-Biosciences

Pre-cut transfer membranes and padding for Western blot transfer procedures. Pre-cut membranes are supplied sandwiched between blotting paper padding. Simply soak the membrane in transfer buffer and assemble with the gel in a transfer cassette. Nitrocellulose (0,22 µm pore size) and PVDF (0,45 µm pore size) membranes are available.

Supplier: Whatman products (Cytiva)

Membrána Nytran® Nylon (Nytran® N) je ideální pro aplikace vyžadující nižší náboj. Je navržena pro Southernův a Northernův blotting, stejně jako přenos kolonií a plaku a "Dot Blots" nebo "Slot Blots" techniky. Nytran® N je kompatibilní s izotopními detekčními metodami.

Supplier: Cytiva

Amersham™ Hybond™-N+ is a positively charged nylon membrane with a binding capacity for nucleic acids up to 600 µg/cm² recommended for use with radioactive or non radioactive chemiluminescence and chemifluorescence detection systems.

Supplier: Cytiva

Amersham™ Hybond™ 0.2 PVDF is a 0,2 μm pore size hydrophobic membrane with high physical strength. This results in significant handling advantages over unsupported nitrocellulose and makes the membrane highly suitable for stripping and reprobing. The small pore size of the membrane minimises 'blow-through' and increases protein binding over a wide range of molecular weights. Ideal for use with standard colorimetric and chemiluminescent detection methods for proteins of

Supplier: Cytiva

Amersham™ Hybond™ SEQ 0.2 PVDF is a 0,2 µm pore size hydrophobic membrane designed specifically for protein sequencing applications. The small pore size of this membrane minimises ‘blow-through’ and increases protein binding over a wide range of molecular weights.

Supplier: Cytiva

Amersham™ Protran® Supported is a robust protein blotting membrane, with 0,2 µm pore size, made of reinforced nitrocellulose for multiple reprobings.

Supplier: Cytiva

Amersham™ Hybond™ LFP 0.2 PVDF is a low fluorescent, hydrophobic membrane, with 0,2 µm pore size, for protein transfer. Amersham™ Hybond™ LFP is optimised for fluorescent detection in Western blotting applications and for use with the Amersham™ ECL Plex™ detection system.

Supplier: Cytiva

Amersham™ Hybond™ P 0,45 PVDF is a 0,45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing. For use in chemiluminescent and fluorescent detection methods for proteins of >Mr 20000.

Supplier: Cytiva

Amersham™ Protran® membranes are manufactured from 100% pure nitrocellulose to ensure the highest binding capacity possible. They are compatible with a variety of detection methods, including isotopic, chemiluminescent, colorimetric and fluorescent. No methanol pre-wetting step is required, making this the membrane of choice for proteins which prefer aqueous environments. Prior to transfer the membrane is simply wetted with water, and then placed in the transfer buffer. No other pretreatment steps are necessary.

Supplier: Cytiva

Amersham™ Protran® Premium nitrocellulose blotting membranes provide excellent sensitivity, resolution and low background in fluorescence Western blotting detection applications.

Supplier: Cytiva

Hybond™ ECL™ gives excellent sensitivity, resolution, and low background for all labelling and detection systems (radioactive, non radioactive, and chromogenic), especially when used with ECL™, ECL™ Prime, and ECL™ Plex Western Blotting Detection kits and reagents. Special packaging features include interleaf on both sides of the membrane and air-tight resealable aluminium bag with label space for marking the 'opened on' date.

Supplier: MILLIPORE

Web-supported positively charged nylon transfer membrane for Western blotting with chemiluminescence, radioactive and fluorescent detection methods.

Supplier: MILLIPORE

Immobilon®-P Blotting Sandwich Transfer Membranes

Supplier: MILLIPORE

Immobilon-NC (HATF) membrane is composed of a mixed cellulose esters matrix. It is devoid of surfactants that may interfere with cell wall integrity during cell growth

Supplier: MILLIPORE

These Immobilon® PVDF membranes, each optimised for different protein blotting applications.

Supplier: Sartorius

These nitrocellulose blotting membranes are ideal for Western blotting, DNA blotting, as well as dot or slot blots. They are optimally adapted to all protein blotting systems, such as electro transfer, semi-dry or simple capillary blotting. A high protein binding capacity is combined with low background to give good blocking possibilities. High membrane stability ensures easy handling of the blotting membrane, even when wet.