Hledali jste: Testy proteinové
Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.
Pierce™ Coomassie Plus (Bradford) Protein Assay Kit
Supplier: Thermo Fisher Scientific
The Pierce™ Coomassie Plus Protein assay is a quick and ready-to-use, reducing agent-compatible, improved Bradford assay to quickly measure (A595 nm) total protein concentration compared to a protein standard.
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Phosphoprotein phosphate estimation assay Kit, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Phosphoprotein Phosphate Estimation Assay Kit enables classification and identification of proteins as phosphorylated (serine and threonine), as well as semi-quantitative assessment of the phosphorylation level.
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Horseradish Peroxidase, Biotinylated, Pierce™
Supplier: Thermo Fisher Scientific
Biotinylated horse radish peroxidase is used as control or signal amplification in IHC to amplify the signal of biotinylated primary antibodies using the avidin-biotin complex staining method.
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Amplite® Fluorimetric Biotin Quantitation Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. Amplite® Fluorimetric Biotin Quantitation Kit uses Biotinylite™ Green, a fluorogenic biotin sensor.
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ReadiUse™ TCEP Removal Solution
Supplier: AAT BIOQUEST
TCEP is a common reducing reagent widely used to break disulfide bonds within and between proteins.
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GST protein interaction pull down kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ GST Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with GST-tagged fusion proteins.
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Amplite® Rapid Colorimetric Biotin Quantitation Kit
Supplier: AAT BIOQUEST
Optimised to use with Nanodrop. Amplite™ colorimetric biotin quantitation kit is designed to quantify the number of biotins attached to a protein or other macromolecule with a nanodrop spectrometer.
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Protein Assay, Protn-Latex™
Supplier: G-Biosciences
A simple and reliable method for estimation of protein contamination in latex. Detects as low as 25 to 40 μg protein/gm latex (or 25 to 50 ppm). Protein solutions applied to the test strips form compact and symmetrical spots, their diameter being directly proportional to protein concentration. Protn-Latex™ latex protein extraction buffer extracts protein from the latex sample, which is then applied to the test strips and rapidly developed. By measuring the Ø of protein spots with the Protn-Latex™ card, the range of protein concentration in the sample can be determined.
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Quantitative colorimetric peptide assay, Pierce™
Supplier: Thermo Fisher Scientific
Thermo Scientific™ Pierce™ Quantitative Colorimetric Peptide Assay is an easy-to-use colorimetric microplate assay designed specifically to improve the sensitivity and reproducibility of quantitation of peptide mixtures.
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Streptavidin poly-HRP conjugate, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Streptavidin Poly-HRP conjugate is biotin-binding protein conjugated with polymers of horseradish peroxidase, enabling signal amplification and detection of biotinylated antibodies for IHC and other methods.
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Glutathione Fluorometric Assay Kit
Supplier: Abcam
Simple assay to detect total glutathione.
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Biotin labelling kit
Supplier: Abnova
Biotin Labelling Kit is used for simple and rapid preparation of biotin-labeled antibodies and proteins.
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2-D Quant kit
Supplier: Cytiva
2-D Quant Kit is designed for the accurate determination of protein concentration in samples prepared for electrophoresis techniques such as 2-D electrophoresis, SDS-PAGE, or IEF.
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Histone, from calf thymus, MP Biomedicals
Supplier: MP Biomedicals
Histones are soluble in water (10 mg/ml) or 0,5 N HCl (10 mg/ml), yielding a clear to hazy solution depending on the fraction. They are soluble in 6 M urea (4 to 10 mg/ml), but this will denature the histones.