Hledali jste: Enzymatická stanovení
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Amplite® Ethanol Quantitation Kit
Supplier: AAT BIOQUEST
The ability to rapidly perform quantitative measurements of ethanol is highly desirable in life science research, clinical evaluations, and food and drug industries.
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Amplite® Red Peroxidase Substrate
Supplier: AAT BIOQUEST
Amplite® Red is a sensitive fluorogenic peroxidase substrate that generates a highly red fluorescent product that has maximum absorption of 571 nm and maximum emission of 585 nm.
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Amplite® Fluorimetric Aldehyde Quantitation Kit
Supplier: AAT BIOQUEST
The formation, reactivity and toxicity of aldehydes originating from the peroxidation of lipids of cellular membranes have received great attention in recent years.
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Amplite® Fluorimetric NADH Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
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Amplite® Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
Our Amplite® Fluorometric 20S Proteasome Assay Kit is a homogeneous fluorescent assay that measures the chymotrypsin-like protease activity associated with the proteasome complex either in cultured cells or cell lysates.
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Amplite® Fluorimetric α-Ketoglutarate Quantitation Kit
Supplier: AAT BIOQUEST
Alpha-ketoglutarate (α-ketoglutarate) is a key molecule in the Krebs cycle determining the overall rate of the citric acid cycle of the organism.
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Amplite® Fluorimetric Calcium Quantitation Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Calcium is essential for all living organisms, particularly in cell physiology, where movement of the calcium ion Ca²⁺ into and out of the cytoplasm functions as a signal for many cellular processes.
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Amplite® Rapid Colorimetric PE-Maleimide Quantitation Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® rapid colorimetric PE-maleimide quantitation kit facilitates the quantification of maleimide groups on maleimide-activated PE proteins using a proprietary sensor, Maleimide 680™, which has an absorption peak at ~680 nm.
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Amplite® Colorimetric Asparaginase Activity Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® colorimetric asparaginase activity assay kit provides a simple and straightforward procedure for measuring asparaginase activity in a variety of biological samples.
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Pierce™ Gaussia Luciferase Flash Assay Kit
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Gaussia Luciferase Flash Assay Kit provides researchers with the most sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.
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Peroxide assay kit, PEROXsay™
Supplier: G-Biosciences
PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe²⁺+) ions to ferric (Fe³⁺) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH of the assay allows the ferric (Fe³⁺) ion to complex with the colorimetric reagent, resulting in a change in absorbance that is proportional to the peroxide concentration.
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SAM Methyltransferase assay, SAMfluoro™
Supplier: G-Biosciences
The SAMfluoro™ assay is a fluorescent, continuous enzyme coupled assay that can continuously monitor SAM-dependent methyltransferases without the use of radioactive labels or endpoint measurements.
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Serine hydrolase probes, ActivX™ FP
Supplier: Thermo Fisher Scientific
The ActivX® FP serine hydrolase probes enable selective labeling and enrichment of active serine hydrolases. Fluorophosphonate (FP) probes specifically label serines of enzymatically active serine hydrolases. FP probes are also effective for screening small molecule inhibitors against enzymes derived from cell lysates, subcellular fractions, tissues and recombinant proteins. Depending on the active-site probe tag group and the enzyme class to be profiled, active site-labeled enzymes are detected and quantified by Western blot, fluorescent gel imaging or mass spectrometry.
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ONPG assay kit
Supplier: OZ BIOSCIENCES
ONPG assay kit is ideal to quantify high expression level of beta-Gal.
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Amplite® Colorimetric Phosphofructokinase (PFK) Activity Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® Colorimetric Phosphofructokinase (PFK) Activity Assay Kit provides a simple and direct procedure for precise measurement of PFK enzyme activity in various biological samples.
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Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*
Supplier: AAT BIOQUEST
Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.
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Amplite® Colorimetric Xanthine Oxidase Assay Kit
Supplier: AAT BIOQUEST
Xanthine oxidase (XO)is an enzyme that catalyzes the oxidation of hypoxanthine to xanthine and can further catalyse the oxidation of xanthine to uric acid.
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Amplite® Human Serum Albumin (HSA) Site I Binding Assay Kit
Supplier: AAT BIOQUEST
Human serum albumin (HSA) is one of the most important carriers for acidic drugs in human plasma and has been shown to bind a large number of different compounds in a reversible manner.
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Amplite® Rapid Colorimetric Maleimide Quantitation Kit
Supplier: AAT BIOQUEST
A variety of crosslinking reagents with a maleimide group are widely used for crosslinking proteins to proteins, or proteins to other biomolecules.
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Amplite® Fluorimetric Fluorescamine Protein Quantitation Kit *Blue Fluorescence*
Supplier: AAT BIOQUEST
Fluorescamine is intrinsically non-fluorescent but reacts rapidly with primary aliphatic amines, including those in peptides and proteins, to yield a blue-green-fluorescent derivative.
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Amplite® Fluorimetric Peroxynitrite Quantification Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
Peroxynitrite (ONOO-) is a strong oxidizing species and a highly active nitrating agent.
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Amplite® Fluorimetric Acetylcholine Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Acetylcholine and its metabolites are needed for three main physiological purposes: structural integrity and signaling roles for cell membranes, cholinergic neurotransmission (acetylcholine synthesis), and as a major source for methyl groups via its metabolite, trimethylglycine (betaine) that participates in the S-adenosylmethionine synthesis pathways.
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Amplite® Colorimetric Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® colorimetric GAPDH activity assay kit is a sensitive and simple tool for monitoring GAPDH activity based on a coupled enzymatic reaction catalysed by GAPDH.
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Screen Quest™ Fluorimetric Neutrophil Elastase Inhibitor Screening Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Screen Quest™ fluorimetric neutrophil elastase inhibitor screening kit offers a fast, sensitive, and high-throughput method for screening and characterising potential inhibitors of NE.
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Amplite® Fluorimetric D-Lactate Assay Kit
Supplier: AAT BIOQUEST
Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.
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Amplite® Fluorimetric L-Aspartate (Aspartic Acid) Assay Kit
Supplier: AAT BIOQUEST
Aspartate (or Aspartic acid) is a negatively charged, polar amino acid.
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Amplite® Fluorimetric Beta-Hydroxybutyrate (Ketone Body) Assay Kit
Supplier: AAT BIOQUEST
Ketone bodies are produced by the liver and used peripherally as an energy source when blood glucose levels drop.
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Amplite® Colorimetric Glycerol 3-Phosphate (G3P) Assay Kit
Supplier: AAT BIOQUEST
Glycerol 3-Phosphate (G3P) is an important intermediate in glycolysis metabolic pathway.
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Amplite® Colorimetric Xanthine Assay Kit
Supplier: AAT BIOQUEST
Xanthine is a purine base found in most human body tissues and fluids.
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Amplite® Fluorimetric Xanthine Assay Kit
Supplier: AAT BIOQUEST
Xanthine is a purine base found in most human body tissues and fluids.