Hledali jste: Enzymatická stanovení

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Choline and its metabolites play an important role in the structural integrity and signaling of cell membranes and cholinergic neurotransmission (acetylcholine synthesis).

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Caspases play important roles in apoptosis and cell signaling.

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Aspartate aminotransferase (AST), also called serum glutamic oxaloacetic transaminase (GOT), is a member of transferase family.

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Alanine aminotransferase (ALT), also called serum glutamate pyruvic transaminase (GPT), is a member of transferase family.

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Alanine aminotransferase (ALT), also called serum glutamate pyruvic transaminase (GPT), is a member of transferase family.

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Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells.

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Glutamate oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-NH₂ group of donors with oxygen as an acceptor.

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When cells are exposed to increased levels of oxidative stress, GSSG will accumulate and the ratio of GSH to GSSG will decrease.

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Formaldehyde is a naturally occurring substance.

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Nicotinamide adenine dinucleotide (NAD⁺) and nicotinamide adenine dinucleotide phosphate (NADP⁺) are two important cofactors found in cells.

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Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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Horseradish Peroxidase (HRP) is a small molecule (MW ~40 Kd) that is widely used in a variety of biological detections.

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The traditional BCA protein assay is widely used for quantifying proteins.

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Site-selective modification of proteins is of major interest in chemical biology.

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Glucose, a monosaccharide, is the most important carbohydrate in biology.

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Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.

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Glycerol is a precursor for synthesis of triglycerides and phospholipids in the liver and adipose tissue.

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Oxaloacetate is an important part of citric acid cycle, where it reacts with Acetyl-CoA to form citrate.

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A variety of substrates are available for detecting peroxidase activity in ELISA assays. colourimetric HRP substrates (e.g., TMB, OPD and ABTS) have been widely used for years. Each of these substrates varies greatly with respect to its performance characteristics such as detection sensitivity, working range and attainable signal-to-noise ratios. Amplite® Blue peroxidase substrate is a soluble fluorogenic substrate for detection of peroxidase activity. Amplite® Blue allows rapid HRP detection assays to be performed with greater sensitivity than the colourimetric HRP substrates (such as TMB and OPD). The fluorescent product of Amplite® Blue does not photobleach. Amplite® Blue exhibits a flat baseline in assays, which facilitates low-level detection sensitivity and allows for high signal-to-noise ratios.

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The formation, reactivity and toxicity of aldehydes originating from the peroxidation of lipids of cellular membranes have received great attention in recent years.

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Mercuric ion is an essential metal ion that plays an important role in a number of biological processes.

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Butyrylcholinesterase (EC 3.1.1.8; BChE), also known as pseudocholinesterase or plasma cholinesterase, is mainly synthesised in liver and present in blood.

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Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.

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The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.

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Lysyl oxidase (LOX) is an extracellular enzyme that catalyses formation of aldehydes from lysine residues in collagen and elastin precursors.

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Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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Glutathione peroxidase is an enzyme family with peroxidase activity to protect the organism from oxidative damage.

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Glucose-6-phosphate dehydrogenase (G6PD) catalyses the conversion of glucose-6-phosphate to 6-phosphoglucono-δ-lactone, the first and rate-limiting step in the pentose phosphate pathway.