Hledali jste: Enzymatická stanovení
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Amplite® Fluorimetric Malondialdehyde (MDA) Quantitation Kit
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Malondialdehyde (MDA) is one of the natural byproducts during lipid peroxidation.
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Amplite® Universal Fluorimetric MMP Activity Assay Kit *Red Fluorescence*
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The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.
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Amplite® Fluorimetric Acidic Sphingomyelinase Assay Kit *Red Fluorescence*
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Sphingomyelinase (SMase) is an enzyme that is responsible for cleaving sphingomyelin (SM) to phosphocholine and ceramide.
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Amplite® Colorimetric D-Lactate Dehydrogenase (LDH) Assay Kit
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Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.
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Amplite® Fluorimetric Glucose Oxidase Assay Kit *Red Fluorescence*
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The glucose oxidase is a dimeric protein that catalyses the oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolyzed to gluconic acid.
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Amplite® Colorimetric Antioxidant Activity Assay Kit
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Reactive oxygen species (ROS) are essential in cell signaling and maintaining physiological cell metabolism.
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Amplite® Fluorimetric Glucose Quantitation Kit
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Glucose, a monosaccharide, is the most important carbohydrate in biology.
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Amplite® IR
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Our Amplite® IR is a fluorogenic peroxidase substrate that generates near infrared fluorescence upon reaction with peroxidase and H2O2.
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Amplite® Fluorimetric Glutamic Acid Assay Kit *Red Fluorescence*
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Glutamic acid is one of the 20 proteinogenic amino acids.
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Amplite® Fluorimetric Glutathione Assay Kit *Green Fluorescence*
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The monitoring of reduced and oxidised glutathione (GSH) in biological samples is essential for evaluating the redox and detoxification status of cells and tissues in relation to the protective role of glutathione against oxidative and free-radical-mediated cell injury.
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Amplite® Fluorimetric NADP/NADPH Ratio Assay Kit *Red Fluorescence*
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Nicotinamide adenine dinucleotide (NAD⁺) and nicotinamide adenine dinucleotide phosphate (NADP⁺) are two important cofactors found in cells.
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ReadiUse™ NADPH Regenerating Kit
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NADPH provides the reducing equivalents for biosynthetic reactions and for oxidation-reduction involved in protection against the toxicity of ROS (reactive oxygen species).
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Amplite® Fluorimetric NADP Assay Kit *Blue Fluorescence*
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Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors for many enzyme reactions found in living cells.
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Amplite® Fluorimetric Peroxidase (HRP) Assay Kit *Red Fluorescence*
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Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.
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Amplite® Luminometric Peroxidase (HRP) Assay Kit
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Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.
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Amplite® Colorimetric Endotoxin Detection Kit
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Lipopolysaccharide (LPS), also known as endotoxin, is the major component of the outer membranes of Gram-negative bacteria.
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Amplite® Colorimetric Beta-Galactosidase Assay Kit
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E. coli beta-galactosidase is a 464 kD tetramer.
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Amplite® Colorimetric L-Lactate Assay Kit
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Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.
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Amplite® Colorimetric Lipase Activity Assay Kit
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Designed for consistent and dependable results. The Amplite® colorimetric lipase activity assay kit offers a straightforward and efficient protocol for quantifying lipase activity.
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Beadlite™ Rapid Colorimetric Thiol Quantitation Kit for Nanoparticles
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Designed for consistent and dependable results. Beadlite™ Rapid Colorimetric Thiol Quantitation kit provides a robust method to quantify thiol-functionalised nanoparticle with our proprietary Thiolite™ 490 that has its maximum absorbance at ~490 nm.
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Amplite® Fluorimetric Glucose and Sucrose Assay Kit
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Designed for consistent and dependable results. The Amplite® fluorimetric glucose and sucrose assay kit offers a convenient method for measuring glucose and sucrose levels in various biological samples, such as serum, plasma, body fluids, food, tissue extracts, and growth medium.
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Amplite® Fluorimetric Protein Quantitation Kit *Orange Fluorescence*
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Protein quantification is an essential task in protein purification, electrophoresis, cell biology, molecular biology and other research applications.
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MMP-3 Green™ Substrate
Supplier: AAT BIOQUEST
The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.
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Amplite® Fluorimetric Sphingomyelinase Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Five types of sphingomyelinase (SMase) have been identified based on their cation dependence and pH optima of action.
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Amplite® Fluorimetric Xanthine Oxidase Assay Kit *Red Fluorescence*
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Xanthine oxidase (XO) is an enzyme that catalyses the oxidation of hypoxanthine to xanthine and can further catalyze the oxidation of xanthine to uric acid.
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Amplite® Fluorimetric Acetylcholinesterase Assay Kit *Green Fluorescence*
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Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group.
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Amplite® Colorimetric Peroxidase (HRP) Assay Kit *Blue Color*
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Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.
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Amplite® Fluorimetric Tyrosinase Assay Kit
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Tyrosinase is an enzyme expressed across a vast range of species from bacteria and fungi to animals.
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Amplite® Colorimetric L-Lactate Dehydrogenase (LDH) Assay Kit
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Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.
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Amplite® Fluorimetric Pyruvate Assay Kit
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Pyruvate is an important chemical compound in intracellular metabolic pathways.