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553 výsledky pro Buněčná stanovení

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Buněčná stanovení

Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signalling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.

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ATP-Glo™ Bioluminometric cell viability assay

ATP-Glo™ Bioluminometric cell viability assay

Supplier: Biotium

ATP-Glo™ Bioluminometric Cell Viability Assay offers a highly sensitive assay for quantifying ATP. The homogeneous assay involves a single addition of ATP-Glo™ Detection Cocktail directly to cells in culture medium.

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CF® Dye Dextran 70000 MW, anionic and fixable

CF® Dye Dextran 70000 MW, anionic and fixable

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 70,000 MW, and contains a formaldehyde-fixable free-amine group.

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Apoptosis and necrosis quantitation kits

Apoptosis and necrosis quantitation kits

Supplier: Biotium

Apoptosis and necrosis are two processes by which cells die. Apoptosis is an active, regulated disassembly of the cell from within. During apoptosis, phosphatidylserine is translocated from the inner to the outer surface of the cell, allowing the dying cell to be engulfed by phagocytic cells. Annexin V is a 35 kD Ca2+-dependent phospholipid binding protein with a high affinity for phosphatidylserine.

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Phagocytosis assay kit (IgG FITC)

Supplier: Cayman Chemical

Phagocytosis Assay Kit (IgG FITC) for the identification of factors regulating the phagocytic process in vitro.

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CF® Dye Dextran 10000 MW, anionic and fixable

CF® Dye Dextran 10000 MW, anionic and fixable

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 10000 MW, and contains a formaldehyde-fixable free-amine group.

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CF® Dye Dextran 250000 MW, anionic and fixable

CF® Dye Dextran 250000 MW, anionic and fixable

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 250,000 MW, and contains a formaldehyde-fixable free-amine group.

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Triglyceride colorimetric assay kit

Supplier: Cayman Chemical

Triglyceride Colorimetric Assay Kit is a simple, reproducible and sensitive tool for assaying triglycerides in plasma, serum, cell lysates and tissue homogenate samples.

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NF-κB (p65) transcription factor assay kit

NF-κB (p65) transcription factor assay kit

Supplier: Cayman Chemical

NF-κB (p65) Transcription Factor Assay Kit is a 96-well assay for measurement of NF-κB (p65).

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ViaFluor® 488 live cell microtubule stain 1000X in DMSO

Supplier: Biotium

Stain microtubules in live cells with green fluorescence.

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Steady-Luc™ Firefly HTS assay kit (lyophilised)

Steady-Luc™ Firefly HTS assay kit (lyophilised)

Supplier: Biotium

Firefly luciferase is widely used as a transcriptional reporter gene for research and drug screening. The Steady-Luc™ HTS kit can be used with luminescence plate readers without sample injection systems or for high-throughput screening.

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LDH Cytotoxicity Assay Kit

LDH Cytotoxicity Assay Kit

Supplier: Cayman Chemical

For measurement of LDH activity as an indicator of cell death.

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Blue IHC chromogen (AP), HIGHDEF®

Supplier: ENZO LIFE SCIENCES

Blue IHC chromogen (AP) is intended to be used in conjunction with alkaline phosphatase (AP)-based immunostaining or in situ hybridisation systems.

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Membrane potential detection kit, MITO-ID®

Membrane potential detection kit, MITO-ID®

Supplier: ENZO LIFE SCIENCES

MITO-ID® membrane potential detection kit monitors energetic status using a simple mix-and-read, no-wash protocol. It measures mitochondrial membrane potential with a cationic dye that fluoresces either green or orange depending upon membrane potential status.

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TOTAL-NUCLEAR-ID® green/red nucleolar/nuclear detection kit

Supplier: ENZO LIFE SCIENCES

The kit contains two proprietary dyes suitable for visualizing nucleoli and nuclei simultaneously in living cells.

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FLUOR DE LYS® HDAC fluorometric cellular activity assay kit

Supplier: ENZO LIFE SCIENCES

The HDAC fluorometric cellular activity assay allows the determination of deacetylase activity within an undisturbed cellular environment and provides accurate activity information that is reflective of endogenous regulation. It also allows the study of the effects of upstream regulators on deacetylase activity and the detection of inhibitors or activators that act indirectly to affect deacetylase activity.

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FLUOR DE LYS® HDAC8 fluorometric drug discovery kit

Supplier: ENZO LIFE SCIENCES

A Fluor-de-Lys® fluorescent assay system. The HDAC8 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC8 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions. The Fluor de Lys® HDAC8 assay is based on the Fluor de Lys® Substrate and Fluor de Lys® Developer combination. The assay procedure has two steps. First, the Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with HDAC6. Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the Fluor de Lys®Developer produces a fluorophore.

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Mitochondria/cytosol fractionation kit

Supplier: ENZO LIFE SCIENCES

For the effective isolation of a highly enriched mitochondrial fraction from the cytosolic fraction of various mammalian cells including both apoptotic an non-apoptotic cells. The enriched mitochondrial and cytosolic fractions can be used to study apoptosis and signal trasduction pathways by detecting translocations of factors of interest between the two fractions by ELISA, Western blot or other assays.

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Myeloperoxidase fluorometric detection kit

Supplier: ENZO LIFE SCIENCES

The Myeloperoxidase fluorometric detection kit is a one-step no wash homogeneous activity assay that is adaptable to HTS applications. This kit offers highly sensitive fluorescent detection and the ability to monitor multiple time points to follow the kinetics of MPO activity.

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FLUOR DE LYS® HDAC fluorometric activity assay kit

Supplier: ENZO LIFE SCIENCES

The FLUOR DE LYS® HDAC fluorometric activity assay kit is a sensitive and convenient alternative to protocols utilizing radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. It is based on the unique FLUOR DE LYS® (Fluorimetric Histone deAcetylaseLysyl) substrate and developer combination and provides an assay that can be carried out in two simple mixing steps, all on the same 96-well plate. Histone deacetylase inhibitors have shown promise as anti-tumor agents and naturally this has stimulated interest in the screening of compounds for HDAC inhibition.

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ER-ID™ red assay kit (GFP-CERTIFIED®)

ER-ID™ red assay kit (GFP-CERTIFIED®)

Supplier: ENZO LIFE SCIENCES

ER-ID® Red assay kit (GFP-CERTIFIED®) contains an endoplasmic reticulum-selective dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining.

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Nitrotyrosine IP Kit

Nitrotyrosine IP Kit

Supplier: Cayman Chemical

For the capture and pulldown of nitrated proteins from cell and tissue lysates.

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Glutathione Cell-Based Detection Kit (Blue Fluorescence)

Glutathione Cell-Based Detection Kit (Blue Fluorescence)

Supplier: Cayman Chemical

For rapid and easy determination of GSH in whole cells.

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Prostaglandin Screening Library III

Prostaglandin Screening Library III

Supplier: Cayman Chemical

For A and J series prostaglandin analog screening.

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Cell counting kit-F

Supplier: ENZO LIFE SCIENCES

Fluorometric kit for simple and accurate cell proliferation and cytotoxicity assays. Advantages includes no radioisotopes, more sensitive than the colorimetric assay, short staining reaction time, no solubilization step, correlates with the [3H]-thymidine incorporation assay.

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Glucose Uptake Cell-Based Assay Kit

Glucose Uptake Cell-Based Assay Kit

Supplier: Cayman Chemical

A cell-based assay for studying modulators of cellular glucose uptake.

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DuraClone Dry Unitized Antibody Panels For Flow Cytometry

DuraClone Dry Unitized Antibody Panels For Flow Cytometry

Supplier: Beckman Coulter

The dry DURAClone antibody panels are designed for clinical research studies. Every assay tube or well contains a layer with the dried-down antibody panel unitized for a single test.

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ALDetect™ (MDA-Specific) Lipid Peroxidation Assay kit

Supplier: ENZO LIFE SCIENCES

Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals, and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides, derived from polyunsaturated fatty acids, are unstable and decompose to form a complex series of compounds. These include reactive aldehydes, of which the most abundant is malondialdehyde (MDA). Therefore, measurement of malondialdehyde is widely used as an indicator of lipid peroxidation. Increased levels of lipid peroxidation products have been associated with a variety of chronic diseases in both humans and model systems.

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LEADING LIGHT® Wnt cell line assay reagents

Supplier: ENZO LIFE SCIENCES

For use with the LEADING LIGHT® Wnt Reporter Cell Line.

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LEADING LIGHT® Wnt reporter cell line

Supplier: ENZO LIFE SCIENCES

Wnt reporter cell line for discovering Wnt pathway modulators.

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ROS-ID™ NO Detection kit

Supplier: ENZO LIFE SCIENCES

The kit is designed to directly monitor real time Nitric Oxide production in live cells by fluorescence microscopy using Nitric oxide Detection Reagent (red fluorescent) as the major component.

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