Hledali jste: Buněčná stanovení
Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signalling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.
ATP-Glo™ Bioluminometric cell viability assay
Supplier: Biotium
ATP-Glo™ Bioluminometric Cell Viability Assay offers a highly sensitive assay for quantifying ATP. The homogeneous assay involves a single addition of ATP-Glo™ Detection Cocktail directly to cells in culture medium.
Expand 3 Items
CF® Dye Dextran 70000 MW, anionic and fixable
Supplier: Biotium
CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 70,000 MW, and contains a formaldehyde-fixable free-amine group.
Expand 6 Items
Apoptosis and necrosis quantitation kits
Supplier: Biotium
Apoptosis and necrosis are two processes by which cells die. Apoptosis is an active, regulated disassembly of the cell from within. During apoptosis, phosphatidylserine is translocated from the inner to the outer surface of the cell, allowing the dying cell to be engulfed by phagocytic cells. Annexin V is a 35 kD Ca2+-dependent phospholipid binding protein with a high affinity for phosphatidylserine.
Expand 6 Items
Phagocytosis assay kit (IgG FITC)
Supplier: Cayman Chemical
Phagocytosis Assay Kit (IgG FITC) for the identification of factors regulating the phagocytic process in vitro.
Expand 1 Items
CF® Dye Dextran 10000 MW, anionic and fixable
Supplier: Biotium
CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 10000 MW, and contains a formaldehyde-fixable free-amine group.
Expand 11 Items
CF® Dye Dextran 250000 MW, anionic and fixable
Supplier: Biotium
CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 250,000 MW, and contains a formaldehyde-fixable free-amine group.
Expand 4 Items
Triglyceride colorimetric assay kit
Supplier: Cayman Chemical
Triglyceride Colorimetric Assay Kit is a simple, reproducible and sensitive tool for assaying triglycerides in plasma, serum, cell lysates and tissue homogenate samples.
Expand 1 Items
NF-κB (p65) transcription factor assay kit
Supplier: Cayman Chemical
NF-κB (p65) Transcription Factor Assay Kit is a 96-well assay for measurement of NF-κB (p65).
Expand 1 Items
ViaFluor® 488 live cell microtubule stain 1000X in DMSO
Supplier: Biotium
Stain microtubules in live cells with green fluorescence.
Expand 2 Items
Steady-Luc™ Firefly HTS assay kit (lyophilised)
Supplier: Biotium
Firefly luciferase is widely used as a transcriptional reporter gene for research and drug screening. The Steady-Luc™ HTS kit can be used with luminescence plate readers without sample injection systems or for high-throughput screening.
Expand 3 Items
LDH Cytotoxicity Assay Kit
Supplier: Cayman Chemical
For measurement of LDH activity as an indicator of cell death.
Expand 2 Items
Blue IHC chromogen (AP), HIGHDEF®
Supplier: ENZO LIFE SCIENCES
Blue IHC chromogen (AP) is intended to be used in conjunction with alkaline phosphatase (AP)-based immunostaining or in situ hybridisation systems.
Expand 1 Items
Membrane potential detection kit, MITO-ID®
Supplier: ENZO LIFE SCIENCES
MITO-ID® membrane potential detection kit monitors energetic status using a simple mix-and-read, no-wash protocol. It measures mitochondrial membrane potential with a cationic dye that fluoresces either green or orange depending upon membrane potential status.
Expand 2 Items
TOTAL-NUCLEAR-ID® green/red nucleolar/nuclear detection kit
Supplier: ENZO LIFE SCIENCES
The kit contains two proprietary dyes suitable for visualizing nucleoli and nuclei simultaneously in living cells.
Expand 1 Items
FLUOR DE LYS® HDAC fluorometric cellular activity assay kit
Supplier: ENZO LIFE SCIENCES
The HDAC fluorometric cellular activity assay allows the determination of deacetylase activity within an undisturbed cellular environment and provides accurate activity information that is reflective of endogenous regulation. It also allows the study of the effects of upstream regulators on deacetylase activity and the detection of inhibitors or activators that act indirectly to affect deacetylase activity.
Expand 1 Items
FLUOR DE LYS® HDAC8 fluorometric drug discovery kit
Supplier: ENZO LIFE SCIENCES
A Fluor-de-Lys® fluorescent assay system. The HDAC8 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC8 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions. The Fluor de Lys® HDAC8 assay is based on the Fluor de Lys® Substrate and Fluor de Lys® Developer combination. The assay procedure has two steps. First, the Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with HDAC6. Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the Fluor de Lys®Developer produces a fluorophore.
Expand 1 Items
Mitochondria/cytosol fractionation kit
Supplier: ENZO LIFE SCIENCES
For the effective isolation of a highly enriched mitochondrial fraction from the cytosolic fraction of various mammalian cells including both apoptotic an non-apoptotic cells. The enriched mitochondrial and cytosolic fractions can be used to study apoptosis and signal trasduction pathways by detecting translocations of factors of interest between the two fractions by ELISA, Western blot or other assays.
Expand 1 Items
Myeloperoxidase fluorometric detection kit
Supplier: ENZO LIFE SCIENCES
The Myeloperoxidase fluorometric detection kit is a one-step no wash homogeneous activity assay that is adaptable to HTS applications. This kit offers highly sensitive fluorescent detection and the ability to monitor multiple time points to follow the kinetics of MPO activity.
Expand 1 Items
FLUOR DE LYS® HDAC fluorometric activity assay kit
Supplier: ENZO LIFE SCIENCES
The FLUOR DE LYS® HDAC fluorometric activity assay kit is a sensitive and convenient alternative to protocols utilizing radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. It is based on the unique FLUOR DE LYS® (Fluorimetric Histone deAcetylaseLysyl) substrate and developer combination and provides an assay that can be carried out in two simple mixing steps, all on the same 96-well plate. Histone deacetylase inhibitors have shown promise as anti-tumor agents and naturally this has stimulated interest in the screening of compounds for HDAC inhibition.
Expand 1 Items
ER-ID™ red assay kit (GFP-CERTIFIED®)
Supplier: ENZO LIFE SCIENCES
ER-ID® Red assay kit (GFP-CERTIFIED®) contains an endoplasmic reticulum-selective dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining.
Expand 1 Items
Nitrotyrosine IP Kit
Supplier: Cayman Chemical
For the capture and pulldown of nitrated proteins from cell and tissue lysates.
Expand 1 Items
Glutathione Cell-Based Detection Kit (Blue Fluorescence)
Supplier: Cayman Chemical
For rapid and easy determination of GSH in whole cells.
Expand 1 Items
Prostaglandin Screening Library III
Supplier: Cayman Chemical
For A and J series prostaglandin analog screening.
Expand 1 Items
Cell counting kit-F
Supplier: ENZO LIFE SCIENCES
Fluorometric kit for simple and accurate cell proliferation and cytotoxicity assays. Advantages includes no radioisotopes, more sensitive than the colorimetric assay, short staining reaction time, no solubilization step, correlates with the [3H]-thymidine incorporation assay.
Expand 2 Items
Glucose Uptake Cell-Based Assay Kit
Supplier: Cayman Chemical
A cell-based assay for studying modulators of cellular glucose uptake.
Expand 1 Items
DuraClone Dry Unitized Antibody Panels For Flow Cytometry
Supplier: Beckman Coulter
The dry DURAClone antibody panels are designed for clinical research studies. Every assay tube or well contains a layer with the dried-down antibody panel unitized for a single test.
Expand 8 Items
ALDetect™ (MDA-Specific) Lipid Peroxidation Assay kit
Supplier: ENZO LIFE SCIENCES
Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals, and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides, derived from polyunsaturated fatty acids, are unstable and decompose to form a complex series of compounds. These include reactive aldehydes, of which the most abundant is malondialdehyde (MDA). Therefore, measurement of malondialdehyde is widely used as an indicator of lipid peroxidation. Increased levels of lipid peroxidation products have been associated with a variety of chronic diseases in both humans and model systems.
Expand 1 Items
LEADING LIGHT® Wnt cell line assay reagents
Supplier: ENZO LIFE SCIENCES
For use with the LEADING LIGHT® Wnt Reporter Cell Line.
Expand 1 Items
LEADING LIGHT® Wnt reporter cell line
Supplier: ENZO LIFE SCIENCES
Wnt reporter cell line for discovering Wnt pathway modulators.
Expand 1 Items
ROS-ID™ NO Detection kit
Supplier: ENZO LIFE SCIENCES
The kit is designed to directly monitor real time Nitric Oxide production in live cells by fluorescence microscopy using Nitric oxide Detection Reagent (red fluorescent) as the major component.