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Buněčná stanovení

Buněčná stanovení

Buněčné testy byly vždy silným nástrojem ve výzkumné laboratoři. Jsou mimořádně všestranné a lze je navrhnout tak, aby měřily prakticky jakoukoli buněčnou nebo biochemickou funkci. Citlivá detekce fluorescenčních sond pomáhá při hodnocení buněčné signalizace a drah buněčné smrti, což je užitečné pro rychlou kvantifikaci životaschopnosti buněk. Buněčné testy lze použít k měření účinků různých ošetření na životaschopnost buněk a k hodnocení toxických účinků zprostředkovaných různými mechanismy.

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CYTO-ID® Autophagy detection kit

Supplier: Enzo Life Sciences

The CYTO-ID® Autophagy detection kit provides a rapid, specific and quantitative approach for monitoring autophagy in live cells by fluorescence microscopy and flow cytometry.

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Nitric oxide reagents and kits

Nitric oxide reagents and kits

Supplier: Biotium

Nitric oxide donors, nitric oxide synthase inhibitors and nitric oxide detection reagents and kits for nitric oxide (NO) studies.

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Red hydrogen peroxide assay kit

Supplier: Enzo Life Sciences

Red Hydrogen Peroxide Assay Kit provides a simple absorbance-based or fluorometric assay to detect and quantify hydrogen peroxide or peroxidase activity in biochemical assays, cell extracts and in live cells. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions. The kit is an optimized ‘mix and read’ assay that is suitable for both 96- and 348-well formats and is compatible with HTS liquid handling.

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SREBP-2 transcription factor assay kit

SREBP-2 transcription factor assay kit

Supplier: Cayman Chemical

96 well assay for measurement of SREBP-2.

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Prostaglandin Screening Library I

Prostaglandin Screening Library I

Supplier: Cayman Chemical

For screening of PGF analogs.

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FLUOR DE LYS®-Green HDAC2 fluorometric drug discovery assay kit

Supplier: Enzo Life Sciences

The HDAC2 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC2 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or kinetic assay of the enzyme under varying conditions.

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NUCLEAR-ID® green cell cycle kit for flow cytometry

Supplier: Enzo Life Sciences

For cell cycle results independent of incubation time, temperature, dye and cell concentrations.

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ROS Detection Cell-Based Assay Kit (DHE)

ROS Detection Cell-Based Assay Kit (DHE)

Supplier: Cayman Chemical

Measure superoxide and hydrogen peroxide levels in living cells.

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Steady-Luc™ Firefly HTS assay kit

Steady-Luc™ Firefly HTS assay kit

Supplier: Biotium

Firefly luciferase is widely used as a transcriptional reporter gene for research and drug screening. The Steady-Luc™ HTS kit can be used with luminescence plate readers without sample injection systems or for high-throughput screening.

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PPARα transcription factor assay kit

PPARα transcription factor assay kit

Supplier: Cayman Chemical

A 96-well assay for measurement of PPARα DNA binding activity.

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Comet SCGE assay kit

Supplier: Enzo Life Sciences

COMET SCGE Assay measures DNA damage by fluorescently detecting the integrity of DNA liberated from cells embedded in low melting point agarose. Upon electrophoresis, fragmented DNA produces a characteristic "comet" shaped tail as small DNA fragments migrate in the gel more rapidly than in-tact genomic DNA.

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Solutions colorantes, Ghost Dye™

Solutions colorantes, Ghost Dye™

Supplier: Tonbo Biosciences

Ghost Dye solutions allow for clear live vs. dead cell discrimination for more precise flow cytometry analysis, and are especially useful for intracellular staining protocols.

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LipidLaunch™ BODIPY SM-102 LNP Kit (Loadable)

Supplier: Cayman Chemical

Cayman’s LipidLaunch™ BODIPY SM-102 LNP kit (loadable) provides cargo-ready, empty, BODIPY-labelled SM-102 LNPs. LNPs are capable of rapid encapsulation of nucleic acids, without the need for incubation steps, enabling subsequent delivery to target cells or other downstream research applications. Also demonstrate very low toxicity compared to traditional lipid-based transfection methods.

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Green detection kit, MITO-ID®

Supplier: Enzo Life Sciences

MITO-ID® Green Detection Kit is a potent mitochondria-selective dye suitable for live, permeabilised and fixed cell staining.

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ViaFluor® 405 live cell microtubule stain 1000X in DMSO

Supplier: Biotium

Stain microtubules in live cells with blue fluorescence.

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ExoBrite™ WGA EV Staining Kits

Supplier: Biotium

ExoBrite™ WGA EV staining kits were designed to overcome some of the challenges of EV detection, particularly in flow cytometry. ExoBrite™ WGA EV stains bind to molecules in the EV membrane for bright, specific staining, with little background.

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