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Supplier: Enzo Life Sciences

A complete colorimetric assay kit for measuring cellular calcineurin (PP2B) phosphatase activity. It employs a convenient 96-well plate format with all reagents necessary for measuring calcineurin (PP2B) phosphatase activity in tissue/cellular extracts PLUS human recombinant calcineurin is included as a positive control.

Supplier: Enzo Life Sciences

For the detection of apoptotic cells by flow cytometry.

Supplier: Enzo Life Sciences

This kit is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy.

Supplier: Enzo Life Sciences

The ROS-ID® Hypoxia/Oxidative Stress Detection Kit is a specific detection system designed for functional detection of hypoxia and oxidative stress levels in live cells (both suspension and adherent) using fluorescent microscopy or flow cytometry.

Supplier: Enzo Life Sciences

The LEADING LIGHT® Wnt Reporter Assay Starter Kit is a cell-based luciferase activity test suitable for 96 well plate format.

Supplier: Enzo Life Sciences

For use with GFP-expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins.

Supplier: Enzo Life Sciences

A Real-time Mitochondrial membrane Potential Assay with Superior Sensitivity. Suitable for high-throughput applications.

Supplier: Enzo Life Sciences

The GOLGI ID® Green assay kit contains a Golgi apparatus-selective dye suitable for live cell, or aldehyde-fixed cell staining. It is more faithfully localized to the target organelle, with minimal staining of the endoplasmic reticulum. Micromolar concentrations of GOLGI ID® Green dye are sufficient for staining mammalian cells, as validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line, Jurkat, canine kidney cell line MDCK, and human bone osteosarcoma epithelial cell line U2OS.

Supplier: Biotium

The Bacteria Viability/Cytotoxicity Assay Kit provides two-colour fluorescence staining of both live bacteria (green) and dead bacteria (red) using two probes, DMAO and EthD-III.

Supplier: Biotium

MTT, XTT, and resazurin are reduced by mitochondrial metabolic activity to yield coloured or fluorescent products, and thus are useful for and assaying cell viability and quantitating cell number. MTT and XTT are reduced to coloured formazin salts that can be measured by absorbance. MTT generates an insoluble formazin salt, requiring cell lysis before the absorbance can be measured, while XTT does not require cell lysis for measurement. Resazurin is a non-fluorescent blue dye that is reduced to the pink fluorescent compound resorufin, which can be measured by fluorescence or absorbance.

Supplier: Biotium

This NucView™488 and RedDot™2 Apoptosis and Necrosis Assay Kit contains NucView™488 caspase-3 substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity. This kit provides a convenient tool for profiling apoptotic and necrotic cell populations by fluorescence microscopy or flow cytometry.

Supplier: Biotium

TUNEL (terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling) is highly selective for the detection of apoptotic cells, but not necrotic cells or cells with DNA strand breaks resulting from irradiation or drug treatment. In this assay, TdT enzyme catalyses the addition of labeled dUTP to the 3’ ends of cleaved DNA fragments. Fluorescent dye-conjugated dUTP can be used for direct detection of fragmented DNA by fluorescence microscopy or flow cytometry.

Supplier: Biotium

CellBrite kits are designed to label cell cytoplasmic membranes with a fluorescent membrane dye pre-dissolved as an optimised staining solution. By combining CellBrite membrane stains, it is possible to label multiple cell populations with distinctive colours, a capability useful for the studies of cell-cell interactions.

Supplier: Enzo Life Sciences

The GFP-Certified™ FluoForte™ Calcium Assay kit provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets.

Supplier: Enzo Life Sciences

For use with the LEADING LIGHT® Wnt Reporter Cell Line.

Supplier: Enzo Life Sciences

This E3 ligase auto-ubiquitinylation kit enables proteins to be tested for ubiquitin E3 ligase activity through assessment of their ability to under auto-ubiquitinylation. Utilizing the first three steps in the ubiquitin cascade the kit facilitates ubiquitinylation of known or putative E3 ligase enzymes followed by Western blot analysis using the highly sensitive reagents provided or using antibodies to the specific protein of interest (user supplied). A high integrity ubiquitin E3 ligase enzyme is also provided for use as a positive control.

Supplier: Enzo Life Sciences

The assay is designed to detect ADP/ATP ratio. The changes in ADP/ATP ratios have been used to conveniently differentiate apoptosis from necrosis.

Supplier: Enzo Life Sciences

This kit provides a rapid assay for nuclear condensation, a prominent hallmark of apoptosis.A highly permeable green-emitting dye for enhanced detection of apoptosis-induced chromatin condensation.

Supplier: Enzo Life Sciences

HIGHDEF® black IHC chromogen (HRP) is intended to be used in conjunction with horseradish peroxidase (HRP)-based immunostaining or in situ hybridisation systems.

Supplier: Enzo Life Sciences

Based on the dye cresyl violet and the caspase 3/7 DEVD peptide recognition sequence, this kit allows the detection of caspases 3 and 7 in living cells by fluorescence microscopy.

Supplier: G-Biosciences

Apoptosis inducers are used for induction of apoptosis in cultured cells. The selection of apoptosis inducers include actinomycin D, camptothecin, cycloheximide, dexamethasone, doxorubicin and etoposide.

Supplier: Cayman Chemical

A convenient method for the direct visualisation of S-glutathionylated proteins in whole (permeabilised) cells.

Supplier: Cayman Chemical

HIF-1α Transcription Factor Assay Kit is a sensitive, non radioactive method for detecting HIF-1α from whole cell lysates.

Supplier: Cayman Chemical

96-Well assay for measurement of p53.

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 3,500 MW, and contains a formaldehyde-fixable free-amine group.

Supplier: Enzo Life Sciences

For the quantitative determination of sPLA2 in culture supernatants, cell lysates, and buffers from any species.

Supplier: Enzo Life Sciences

Simple assay to quantify senescence-associated β-galactosidase activity, in live cells, using flow cytometry or fluorescent microscopy.

Supplier: Enzo Life Sciences

The DPPIV/CD26 Assay kit for biological samples is a complete assay system designed to measure DPPIV activity in biological fluids such as plasma, serum, urine, and saliva. Uses for the kit include correlation of DPPIV activity with disease states or determination of the efficacy of DPPIV inhibitors administered in vivo.

Supplier: Enzo Life Sciences

CYTO-ID can monitor autophagy in live cells and autophagic flux in lysosomally inhibited live cells using a novel dye that selectively labels accumulated autophagic vacuoles.

Supplier: Cayman Chemical

For screening a variety of epigenetic compounds.