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Buněčná stanovení

Buněčná stanovení

Buněčné testy byly vždy silným nástrojem ve výzkumné laboratoři. Jsou mimořádně všestranné a lze je navrhnout tak, aby měřily prakticky jakoukoli buněčnou nebo biochemickou funkci. Citlivá detekce fluorescenčních sond pomáhá při hodnocení buněčné signalizace a drah buněčné smrti, což je užitečné pro rychlou kvantifikaci životaschopnosti buněk. Buněčné testy lze použít k měření účinků různých ošetření na životaschopnost buněk a k hodnocení toxických účinků zprostředkovaných různými mechanismy.

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Red/green (FUN®1), ViaVac™

Red/green (FUN®1), ViaVac™

Supplier: Biotium

A fluorescent vitality dye for yeast, identical in structure to the dye FUN® 1. Living yeast cells show red fluorescent vacuolar tubules.

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Yeast vitality staining kit

Yeast vitality staining kit

Supplier: Biotium

A fluorescent vitality dye for yeast, identical in structure to the dye FUN® 1. Living yeast cells show red fluorescent vacuolar tubules.

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Nrf2 transcription factor assay kit

Nrf2 transcription factor assay kit

Supplier: Cayman Chemical

Nrf2 Transcription Factor Assay kit is a 96-well assay for measurement of Nrf2.

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BCA protein assay kit

Supplier: Thermo Fisher Scientific

BCA Protein Assay Kit can be used to measure very dilute protein concentrations in very small sample volumes. The kit accurately measures protein concentrations from 0,5 to 30 μg/ml in tube assays and 1 to 20 μg/ml in 96- or 384-well plate assays. It is used for studying protein:protein interactions, measuring column fractions after affinity chromatography, estimating percent recovery of membrane proteins from cell extracts and high-throughput screening of fusion proteins.

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CellBrite™ Fix membrane stains, Biotium

CellBrite™ Fix membrane stains, Biotium

Supplier: Biotium

CellBrite™ Fix dyes are fluorogenic membrane dyes that covalently stain the plasma membrane in live cells. They are unique among membrane stains in which they can withstand both fixation and detergent permeabilisation.

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Live-or-Dye™ Yeast fixable live/dead staining kit

Live-or-Dye™ Yeast fixable live/dead staining kit

Supplier: Biotium

The Yeast Live-or-Dye™ Fixable Live/Dead Staining Kit is a fixable, fluorescent assay to determine yeast viability. The kit contains two fluorescent components, Thiazole Orange and Live-or-Dye™ 568/583.

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Firefly luciferase assay kit

Supplier: Biotium

Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues.

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Cell proliferation assay kit, WST-1

Cell proliferation assay kit, WST-1

Supplier: Abnova

WST-1 cell proliferation assay kit provides an easy to use tool for studying induction and inhibition of cell proliferation in any in vitro model.

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ATP assay kit

ATP assay kit

Supplier: Abnova

ATP Assay Kit is for the rapid bioluminescent determination of ATP in biological samples.

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Antioxidant assay kit

Antioxidant assay kit

Supplier: Abnova

Antioxidant Assay Kit is a quantitative colorimetric determination of total antioxidant capacity of cellular samples.

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Malachite green phosphate assay kit

Malachite green phosphate assay kit

Supplier: Abnova

Malachite Green Phosphate Assay Kit provides a fast, reproducible, and non-radioactive method for measuring inorganic free phosphate in aqueous solution.

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Nitric oxide assay kit

Nitric oxide assay kit

Supplier: Abnova

Nitric Oxide Assay Kit for the quantitative colorimetric determination of nitric oxide in cell, food sample, plasma, serum, tissue and urine.

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Methylated DNA capture kit

Supplier: Abnova

Methylated DNA Capture Kit is used to enrich methylated DNA.

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Pyruvate assay kit

Pyruvate assay kit

Supplier: Abnova

Pyruvate Assay Kit is for quantitative colorimetric/fluorimetric pyruvate determination.

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Iron Assay Kit

Supplier: Merck

Iron level in blood is a reliable diagnostic indicator of various disease states. Increased levels of iron concentration in blood are associated with red blood cell destruction, decreased blood cell survival, acute hepatitis, certain sideroblastic anemias, ingestion of iron-rich diets, defects in iron storage (e.g., pernicious anemia). Decreased levels of blood iron may result from insufficient iron ingestion from diets, chronic blood loss pathologies, or increased demand on iron storage as during normal pregnancy.

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Glucose (HK) assay kit

Supplier: Merck

The Glucose (HK) Assay Kit is for the quantitative, enzymatic determination of glucose in food and other material.

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