Buněčná stanovení
Buněčné testy byly vždy silným nástrojem ve výzkumné laboratoři. Jsou mimořádně všestranné a lze je navrhnout tak, aby měřily prakticky jakoukoli buněčnou nebo biochemickou funkci. Citlivá detekce fluorescenčních sond pomáhá při hodnocení buněčné signalizace a drah buněčné smrti, což je užitečné pro rychlou kvantifikaci životaschopnosti buněk. Buněčné testy lze použít k měření účinků různých ošetření na životaschopnost buněk a k hodnocení toxických účinků zprostředkovaných různými mechanismy.
LysoBrite™ Orange
Supplier: AAT Bioquest
Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris.
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PhosphoWorks™ Fluorimetric Pyrophosphate Assay Kit, Blue Fluorescence
Supplier: AAT Bioquest
Pyrophosphate (PPi) is produced by a number of biochemical reactions, such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation by the enzyme adenylate cyclase and the enzymatic activation of fatty acids to form their coenzyme A esters.
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Cell Meter™ Autophagy Assay Kit *Blue Fluorescence*
Supplier: AAT Bioquest
Autophagy is an evolutionarily conserved degradation process that targets long-lived proteins, organelles, and other cytoplasmic components for degradation via the lysosomal pathway.
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Cell Meter™ Fixable Viability Dye, RX780
Supplier: AAT Bioquest
Discrimination and exclusion of dead cells from live cells allows cleaner separation and identification of cell populations.
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Cell Meter™ Fluorimetric Live Cell Cycle Assay Kit *Green Fluorescence Optimised for Flow Cytometry*
Supplier: AAT Bioquest
The Cell Meter™ assay kits are a set of tools for monitoring cell viability and proliferation.
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Cell Meter™ Intracellular NADH/NADPH Flow Cytometric Analysis Kit *Red Fluorescence*
Supplier: AAT Bioquest
The detection of intracellular dihydronicotinamide adenine dinucleotide NADH and its phosphate ester NADPH is important for disease diagnostics and drug discovery.
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Helix Fluor™ 545 Succinimidyl Ester
Supplier: AAT Bioquest
FAM, TET, VIC, JOE, NED, TAMRA and ROX fluorescent dyes are frequently used for labelling oligos.
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G-Biosciences Calcein Release Assay (Cell Viability Assay)
Supplier: G-Biosciences
Calcein AM Cell Viability Assay can be easily adapted to various fluorescence setups such as microplate assays, fluorescence microscope and flow cytometry. The assay is useful for various studies such as cell viability, cell adhesion, chemotaxis, multidrug resistance, apoptosis and cytotoxicity.
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BCA protein assay kit
Supplier: Merck Millipore (Novagen)
BCA Protein Assay is a simple and reliable protein quantification method. It can be used to determine protein concentration in the range of 20-2000 µg/ml in either a standard assay or microassay configuration.
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Firefly luciferase assay kit 2.0
Supplier: Biotium
This kit is designed to measure Renilla luciferase activity in transfected cell reporter gene assays.
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Amyloid plaque staining reagent, Amylo-Glo® RTD™
Supplier: Biosensis
Amylo-Glo RTD reagent is designed to stain amyloid plaques in tissue sections.
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Firefly and Renilla Luciferase single tube Assay
Supplier: Biotium
This kit is designed to measure Firefly and Renilla luciferase activities in transfected cell reporter assays. Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues. Renilla luciferase has been used as a reporter gene for studying gene regulation and function in vitro and in vivo. It commonly is used in multiplex transcriptional reporter assays or as a normalising transfection control for firefly luciferase assays.
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Alamar blue cell viability assay
Supplier: G-Biosciences
AlamarBlue Cell Viability Assay reagent quantitatively measures the proliferation of mammalian cell lines, bacteria and fungi. The dye incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and change colour in response to the chemical reduction of growth medium due to cell growth.
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Yeast viability staining kits
Supplier: Biotium
The Yeast Viability Staining Kit is a fluorescent assay to determine yeast viability. Each kit contains two fluorescent components: CF® Dye-labeled Concanavalin A (Con A) and Live-or-Dye™ viability dye. Biotium offers 3 different color combinations to choose from.
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Amyloid plaque stain reagent, with EtBr counter stain, Amylo-Glo® RTD™
Supplier: Biosensis
Amylo-Glo RTD reagent is designed to stain amyloid plaques in tissue sections.
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Live-or-Dye NucFix™ red staining kit
Supplier: Biotium
Live-or-Dye NucFix™ Red is a red fluorescent dye to covalently label dead cells, allowing cells with permeable plasma membranes to be detected by flow cytometry or fluorescence microscopy.
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