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Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signalling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.
HAT activity assay kit
Supplier: Enzo Life Sciences
Histone acetyltransferases (HATs) have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation. HAT Activity Colorimetric Assay Kit offers a convenient, nonradioactive system for a rapid and sensitive detection of HAT activity in mammalian samples.
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Glucose Uptake Cell-Based Assay Kit
Supplier: Cayman Chemical
A cell-based assay for studying modulators of cellular glucose uptake.
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Prostaglandin Screening Library III
Supplier: Cayman Chemical
For A and J series prostaglandin analog screening.
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NETosis Assay Kit
Supplier: Cayman Chemical
For the measurement of NET-derived neutrophil elastase.
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Glycolysis Cell-Based Assay Kit
Supplier: Cayman Chemical
A colorimetric-based method for detecting L-lactate in culture medium.
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Cell-based assay calcein AM
Supplier: Cayman Chemical
Cellular assay, Cell-based assay calcein AM
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Calcein-AM cell viability assay kits
Supplier: Biotium
Calcein-AM is a non-fluorescent, membrane permeable compound. Esterase activity in the cytoplasm of viable cells converts calcein-AM to the green fluorescent, membrane impermeant compound calcein, which is retained in viable cells with intact plasma membranes. The Viability/Cytotoxicity Assay kit for Animal Live & Dead Cells pairs calcein-AM with the vital dye ethidium homodimer III for quantitation of live and dead cells.
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JC-1 Mitochondrial Membrane Potential Detection Kit, Biotium
Supplier: Biotium
JC-1 dye is used for fluorescence-based measurement of mitochondrial membrane potential changes in cells, a key measure of cell viability.
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CellBrite™ NIR cytoplasmic membrane dyes
Supplier: Biotium
Biotium’s CellBrite™ NIR Cytoplasmic Membrane Dyes are near-infrared lipophilic carbocyanine dyes for labeling cytoplasmic membranes in live or formaldehyde-fixed cells.
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LDH Cytotoxicity WST Assay
Supplier: Enzo Life Sciences
Colorimetric kit for the determination of cytotoxicity through the measurement of lactate dehydrogenase activity.
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Apoptosis inducer set
Supplier: Enzo Life Sciences
The set contains five reagents that induce apoptosis through different mechanisms.
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OXI-TEK TBARS assay kit
Supplier: Enzo Life Sciences
The sensitivity of measuring thiobarbituric acid reactive substances (TBARS) has made this assay the method of choice for screening and monitoring lipid peroxidation, which is a major indication of oxidative stress.
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Caspase-3 assay kits, NucView™ 488
Supplier: Biotium
NucView® 488 caspase-3 substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3 activity within live cells in real time.
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CV-Cathepsin B detection kit
Supplier: Enzo Life Sciences
Based on the dye cresyl violet and the cathepsin B ArgArg peptide recognition sequence, this kit allows the detection of cathepsin B activity in living cells by fluorescence microscopy.
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Annexin V-FITC apoptosis detection kit
Supplier: Enzo Life Sciences
For the detection of apoptotic cells by flow cytometry.
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Calcineurin cellular activity assay kit
Supplier: Enzo Life Sciences
A complete colorimetric assay kit for measuring cellular calcineurin (PP2B) phosphatase activity. It employs a convenient 96-well plate format with all reagents necessary for measuring calcineurin (PP2B) phosphatase activity in tissue/cellular extracts PLUS human recombinant calcineurin is included as a positive control.
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Annexin V-EGFP apoptosis detection kit
Supplier: Enzo Life Sciences
For the detection of apoptotic cells by flow cytometry.
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ROS-ID™ ROS/RNS Detection kit
Supplier: Enzo Life Sciences
This kit is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy.
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Hypoxia/oxidative stress detection kit, ROS-ID®
Supplier: Enzo Life Sciences
The ROS-ID® Hypoxia/Oxidative Stress Detection Kit is a specific detection system designed for functional detection of hypoxia and oxidative stress levels in live cells (both suspension and adherent) using fluorescent microscopy or flow cytometry.
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LEADING LIGHT® Wnt Reporter Assay Starter Kit
Supplier: Enzo Life Sciences
The LEADING LIGHT® Wnt Reporter Assay Starter Kit is a cell-based luciferase activity test suitable for 96 well plate format.
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Lyso-ID® red detection kit (GFP-CERTIFIED®)
Supplier: Enzo Life Sciences
For use with GFP-expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins.
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Mito-ID® Membrane potential cytotoxicity kit for microplates
Supplier: Enzo Life Sciences
A Real-time Mitochondrial membrane Potential Assay with Superior Sensitivity. Suitable for high-throughput applications.
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GOLGI ID® Green assay kit
Supplier: Enzo Life Sciences
The GOLGI ID® Green assay kit contains a Golgi apparatus-selective dye suitable for live cell, or aldehyde-fixed cell staining. It is more faithfully localized to the target organelle, with minimal staining of the endoplasmic reticulum. Micromolar concentrations of GOLGI ID® Green dye are sufficient for staining mammalian cells, as validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line, Jurkat, canine kidney cell line MDCK, and human bone osteosarcoma epithelial cell line U2OS.
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Bacterial Ssaining, viability/cytotoxicity assay kit for bacteria live and dead cells
Supplier: Biotium
The Bacteria Viability/Cytotoxicity Assay Kit provides two-colour fluorescence staining of both live bacteria (green) and dead bacteria (red) using two probes, DMAO and EthD-III.
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Cell viability assay kits
Supplier: Biotium
MTT, XTT, and resazurin are reduced by mitochondrial metabolic activity to yield coloured or fluorescent products, and thus are useful for and assaying cell viability and quantitating cell number. MTT and XTT are reduced to coloured formazin salts that can be measured by absorbance. MTT generates an insoluble formazin salt, requiring cell lysis before the absorbance can be measured, while XTT does not require cell lysis for measurement. Resazurin is a non-fluorescent blue dye that is reduced to the pink fluorescent compound resorufin, which can be measured by fluorescence or absorbance.
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Apoptosis and necrosis assay kit, NucView™488 RedDot™2
Supplier: Biotium
This NucView™488 and RedDot™2 Apoptosis and Necrosis Assay Kit contains NucView™488 caspase-3 substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity. This kit provides a convenient tool for profiling apoptotic and necrotic cell populations by fluorescence microscopy or flow cytometry.
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TUNEL assay kits
Supplier: Biotium
TUNEL (terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling) is highly selective for the detection of apoptotic cells, but not necrotic cells or cells with DNA strand breaks resulting from irradiation or drug treatment. In this assay, TdT enzyme catalyses the addition of labeled dUTP to the 3’ ends of cleaved DNA fragments. Fluorescent dye-conjugated dUTP can be used for direct detection of fragmented DNA by fluorescence microscopy or flow cytometry.
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Cytoplasmic membrane labelling kits, CellBrite™
Supplier: Biotium
CellBrite kits are designed to label cell cytoplasmic membranes with a fluorescent membrane dye pre-dissolved as an optimised staining solution. By combining CellBrite membrane stains, it is possible to label multiple cell populations with distinctive colours, a capability useful for the studies of cell-cell interactions.
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GFP-CERTIFIED® FLUOFORTE® Calcium assay kit
Supplier: Enzo Life Sciences
The GFP-Certified™ FluoForte™ Calcium Assay kit provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets.
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LEADING LIGHT® Wnt cell line medium pack
Supplier: Enzo Life Sciences
For use with the LEADING LIGHT® Wnt Reporter Cell Line.