Hledali jste: Systémy pro spojování a značení proteinů
Protein linking and labelling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colours, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labelling follows a simple protocol of reaction, separation, and then use.
PSA™ iFluor® 555 Imaging Kit with Goat Anti-Rabbit IgG
Supplier: AAT BIOQUEST
iFluor® 555 PSA kit is a much superior replacement for Alexa Fluor 555 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
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PSA™ iFluor® 647 Imaging Kit with Goat Anti-Rabbit IgG
Supplier: AAT BIOQUEST
iFluor® 647 PSA kit is a much superior replacement for Alexa Fluor 647 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
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30 Minute IHC DAB Detection System
Supplier: ANTIBODIES.COM
This 30 Minute IHC DAB Detection System is a powerful intensification system that significantly enhances chromogenic signals. This system can be integrated into standard immunohistochemistry (IHC) staining methods and results in a significant increase in sensitivity compared to standard IHC detection methods, while maintaining similar specificity.
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RNA 3' End biotinylation kit, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ RNA 3' End Biotinylation Kit is optimised for labeling the 3' terminal end of RNA probes to facilitate their use as probes or targets in EMSA and other methods for studying protein-RNA interactions.
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PEGylated SPDP Crosslinkers, Heterobifunctional, Pierce™
Supplier: Thermo Fisher Scientific
Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.
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Antibody labelling kits, Mix-n-Stain™ Digoxigenin
Supplier: Biotium
Mix-n-Stain™ Digoxigenin antibody labelling kits utilise a technology that allows rapid antibody labelling with digoxigenin without a purification step.
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Streptavidin, maleimide-activated
Supplier: Thermo Fisher Scientific
Maleimide-Activated Streptavidin is for directly preparing streptavidin-protein conjugates with proteins, peptides and other molecules that contain a free sulphhydryl (-SH) group. Streptavidin has been maleimide-activated using Sulpho-SMCC, a heterobifunctional crosslinker that contains an N-hydroxysuccinimide ester and a maleimide group. The activated streptavidin presents an available maleimide group that reacts with sulphhydryl-containing molecules.
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Peptide coupling kits, HOOK™
Supplier: G-Biosciences
The HOOK™ Peptide Coupling kits allow researchers to attach peptides to a carrier protein of choice using either primary amines or sulfhydryls in their peptide. The HOOK™ Peptide Coupling kits can be supplied with or without carrier proteins.
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Biotin kits, HOOK™
Supplier: G-Biosciences
HOOK™ Biotin Reagent that conjugates to proteins through amines, sulfhydryls, carboxyls or carbohydrates. The amine and sulfhydryl coupling HOOK™ Biotin Reagents couple directly to the protein through their reactive groups, however the carboxyl coupling HOOK™ Biotin Reagents require a carbodiimide crosslinker and the carbohydrate coupling HOOK™ Biotin Reagents require carbohydrate oxidation before coupling.
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Antibody labelling kits, Mix-n-Stain™ DNP
Supplier: Biotium
Mix-n-Stain™ DNP antibody labelling kits utilise a technology that allows rapid antibody labelling with dinitrophenol without a purification step.
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Fluor-free acids, DyLight™
Supplier: Thermo Fisher Scientific
DyLight™ fluor-free acids are non-activated forms of selected DyLight™ fluorescent dyes for use as controls and calibration factors in fluorescence imaging applications. These non-activated fluorescent dyes contain the native carboxyl group of the standard dye molecule.
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Biotin NTA
Supplier: Biotium
Biotinylated probes for detection of His-tagged proteins immobilised on nitrocellulose membranes.
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FluorSave™ reagent, Calbiochem®
Supplier: Merck Millipore (Calbiochem)
FluorSave™ reagent is an aqueous mounting medium for use in conjunction with fluorescent tracers such as fluorescein, rhodamine and phycobiliprotein probes.
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VeriFluor™ Far-Red Passive Reference Dye
Supplier: Biotium
A far-red passive reference dye for qPCR that frees up the ROX channel for use with JUN™ probes, EvaRuby™ qPCR dye, and other orange dyes.
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Immunostainer enhancer, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Immunostain Enhancer is compatible with fluorescent and chromogenic detection and routinely increases both signal intensity and detection sensitivity.
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High sensitivity NeutrAvidin™ protein-HRP conjugate, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ High Sensitivity NeutrAvidin™-HRP Conjugate is a specially prepared peroxidase conjugated form of avidin biotin binding protein that provides signal amplification like poly-HRP and exceptional storage stability.
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Ghost Dye™ Red 710
Supplier: Tonbo Biosciences
Ghost Dye Red 710 is an amine reactive viability dye, that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications.
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Amersham™ CyDye™ Value Packs (Bis-Reactive NHS Esters)
Supplier: Cytiva
Amersham™ CyDye™ fluors combine exceptional photostability with bright and intense signals, resulting in high sensitivity and a highly reproducible performance at a biologically suitable pH range of 3 to 10.
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Reactive dye 5-packs, Amersham CyDye™
Supplier: Cytiva
Mono-reactive dye pack contains derivatised CyDye™ that carries only one reactive group on each dye molecule for accurate labelling of amine groups, for example on antibodies or oligonucleotides.
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Amersham™ CyDye™ Value Packs (Mono-Reactive Hydrazide), Cytiva
Supplier: Cytiva
Hydrazide esters provide a convenient method for the labelling of free carbonyl groups of glycoproteins and carbohydrates. For maximum convenience, stable active hydrazide esters are supplied and stored as solid material.
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Fluorescent dyes, M series, streptavidin conjugate
Supplier: Abnova
Fluorescent dye, M series, products are designed to be maximally excited by one of the major light sources equipped in flow cytometers. In comparison with phycobiliprotein tandems, Fluorescent dye, M series, possesses better photostability, higher conjugation yields, and little pH sensitivity making it an ideal choice in fluorescence imaging applications.
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Protein labelling Kit, Amersham™ QuickStain
Supplier: Cytiva
The Amersham™ QuickStain Kit contains a Cy™5 fluorophore and labeling buffer for easy detection of proteins in SDSPAGE gels and on Western blotting membranes. The ready to use Cy5 N-hydroxysuccinimide (NHS) ester and Tris labeling buffer ensure robust and consistent labeling for detection of proteins in diverse samples.
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Amersham Cy3 a Cy5 Maleimid Mono-Reactive Dye 5-packs, Cytiva
Supplier: Cytiva
Mono-functional maleimides are particularly suitable for the selective labelling of molecules containing free sulfhydryl groups, such as cysteine residues in proteins and peptides and oligonucleotides.
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Mono-Reactive NHS Ester dye packs, Amersham CyDye™
Supplier: Cytiva
Mono-reactive dye pack contains derivatised CyDye™ that carries only one reactive group on each dye molecule for targeted and specific labelling of amine groups, for example on antibodies, peptides or oligonucleotides.
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CyDye Antibody Labeling Kits
Supplier: Cytiva
CyDye Fluorescent Dyes are supplied as highly water soluble, reactive NHS esters for easy conjugation to biomolecules containing aliphatic amino groups.
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Amersham CyDye DIGE fluors
Supplier: Cytiva
CyDye™ DIGE fluors are available as minimal and saturation labeling dyes. The minimal dyes are intended for general 2-D application use where sufficient amounts of sample are available. The saturation dyes from the Scarce Sample Labeling Kit are designed to be used for applications where only small amounts of sample are available, for example in Laser Capture Microdissection.
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Monofunctional maleimide fluors, CyDye™
Supplier: Cytiva
Cy3 and Cy5 monofunctional maleimides are particularly suitable for the selective labelling of molecules containing free sulfhydryl groups, such as cysteine residues in proteins and peptides and oligonucleotides.
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Amersham™ CyDye™ Fluorescent Nucleotides
Supplier: Cytiva
Fluorescent nucleotides for labelling DNA and RNA probes, especially during multi-colour analysis in microarrays, FISH, chromosome identification, whole chromosome painting, karyotyping, and gene mapping.
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Labeling dye, Atto 488, Sigma-Aldrich®
Supplier: Merck
Atto 488 is a labeling dye with high molecular absorption (90000) and quantum yield (0,80) as well as sufficient stokes shift between excitation and emission maximum. It is optimised for excitation with an argon laser, and is characterised by high photostability.
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Atto 647N-biotin, BioReagent
Supplier: Merck
Designed for high sensitivity applications, including single molecule detection. These labels have rigid structures that do not show any cis-trans-isomerisation and display exceptional intensity with minimal spectral shift on conjugation.