Hledali jste: Systémy pro spojování a značení proteinů
Protein linking and labelling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colours, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labelling follows a simple protocol of reaction, separation, and then use.
VWR® Antibody signal enhancer
Supplier: VWR Chemicals
Antibody Signal Enhancer is a Western blotting antibody diluent that increases intensity of protein detection up to 5-fold compared to conventional blotting.
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Antibody labelling kits, Mix-n-Stain™ CF™ Dye
Supplier: Biotium
Label your antibody with one of our superior CF® Dyes in 30 minutes without a purification step. Labelling tolerates many common buffer components including BSA and ascites.
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CF™ dye bovine serum albumin conjugates
Supplier: Biotium
Fluorescent bovine serum albumin (BSA) conjugates are commonly used as tracers in applications where physical dimensions are important. The conjugates can also be used for quantitative studies of electroporation, measurement of plasma volume in rats, and endocytosis studies. CF™ dye BSA conjugates are labelled with fluorescent CF™ dyes, which offer advantages in brightness and photostability compared to other fluorescent dyes.
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femtoCHROMO™- AP Substrate for chromogenic detection
Supplier: G-Biosciences
A ready to use modified BCIP (5-Bromo-4-Chloro-3’-Indolyphosphate p-Toluidine salt) and NBT (Nitro-Blue Tetrazolium Chloride) substrate that generates a black-purple insoluble precipitate in the presence of alkaline phosphatase.
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ZnAF-2.tetrahydrochloride
Supplier: ENZO LIFE SCIENCES
Fluorescent probe for Zn2+
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SeramunBlock
Supplier: Apollo Scientific
Combined Stabiliser/ blocking solution for solid phase immobilised proteins effecting protein stabilisation and blocking of free surfaces.
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ExoBrite™ EV Surface Stain Sampler Kit
Supplier: Biotium
The ExoBrite™ EV Surface Stain Sampler Kit, Green was developed to offer each of Biotium’s ExoBrite™ EV Surface Stains (CTB, WGA, and Annexin V) for assessing which stain offers the best coverage for the EV samples of interest.
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Nuclear-ID™ red/green cell viability reagent
Supplier: ENZO LIFE SCIENCES
Cell viability dye
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Chemiluminescent labelling kit
Supplier: ENZO LIFE SCIENCES
A complete kit for the labeling of antibodies, proteins, peptides, and nucleic acids with Acridinium NHS Ester.
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Aqua 431 dUTP
Supplier: ENZO LIFE SCIENCES
Aqua 431 [DEAC] dUTP is a coumarin derivative that can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labelled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labelling, cDNA labelling and 3’-end labelling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Aqua 431 dUTP can also be used for multicolor fluorescence labelling.
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Red 580 dUTP
Supplier: ENZO LIFE SCIENCES
Red 580 [5-ROX] dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labelled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labelling, cDNA labelling and 3’-end labelling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Red 580 dUTP can also be used for multicolor fluorescence labelling.
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Bungarotoxins and fluorescent bungarotoxin conjugates
Supplier: Biotium
Bungarotoxins are venom toxins from the many-banded krait (Bungarus Multicinctus), which inhibit neurotransmission at the neuromuscular junction. Alpha-bungarotoxin is a potent inhibitor for the motor endplate acetylcholine receptor (Kd = 1 nM to 1 pM). Beta-bungarotoxin contains a small subunit that blocks voltage-gated K+ channels on the presynaptic membranes, and a large subunit that has phospholipase A2 activity. Fluorescent conjugates of alpha-bungarotoxin can be used to label acetylcholine receptors in skeletal muscle endplates.
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Antibody labelling kits, Mix-n-Stain™
Supplier: Biotium
Mix-n-Stain™ antibody labelling kits utilise a technology that allows rapid antibody labelling without a purification step.
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CF® Dye BCN
Supplier: Biotium
CF® Dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® Dye BCN conjugates for intracellular labelling reactions are also available.
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CF®DI Aminooxy
Supplier: Biotium
CF®DI aminooxy dyes react with aldehyde/ketone groups on proteins or other biomolecules to form a stable oxime bond. The CF®DI dyes (CF®488DI, CF®555DI and CF®647DI) are designed to match each other for molecular weight and charge. The molecular weights of the same protein conjugated to different CF®DI dye colors are close to each other and the pl of the labeled proteins remains the same.
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CF™ dye picolyl azide
Supplier: Biotium
CF® dye picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency. The selected CF® dyes are exceptionally bright and photostable, making them the perfect dye for fluorescence detection.
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Streptavidin conjugates
Supplier: Biotium
A high quality biotin-binding protein conjugated to Biotium's signature bright and photostable fluorescent CF® dyes, and a selection of other labels.
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HRP-conjugate stabiliser, SeramunStab® Stplus
Supplier: Apollo Scientific
Liquid product colourless to slightly yellow or greenish, odourless, pH 7,3 to 7,5. Stabiliser for conjugates with monomeric HRP.
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Single-round RNA amplification and biotin labeling system
Supplier: ENZO LIFE SCIENCES
The Single-Round RNA Amplification and Biotin Labeling System provides an optimized protocol and reagents for the production of biotin-labeled antisense RNA (aRNA) from total cellular RNA samples in less than 24 hours for array analysis.
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Nuclear-ID™ Blue/Green cell viability reagent
Supplier: ENZO LIFE SCIENCES
Nucleus dye
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Thiol-reactive dyes and reagents
Supplier: Biotium
MTS, TS and maleimide, and other thiol-modifying reagents and reactive fluorescent dyes.
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Biotin conjugation estimation, HOOK™ BiotinQuant
Supplier: G-Biosciences
HOOK™ BiotinQuant is designed for the estimation of biotin conjugation. It measures biotin using HABA [4’-hydroxyazobenzene-2-carboxylic acid] dye. HABA binds with avidin at the biotin-binding site.
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Tyramide amplification kit with HRP streptavidin and CF® dye or biotin tyramide
Supplier: Biotium
Kits for increasing immunofluorescence sensitivity using HRP-catalysed tyramide signal amplification with the choice of next-generation fluorescent CF® dyes or biotin.
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Tyramide amplification kit with HRP goat, anti-mouse and CF® dye or biotin tyramide
Supplier: Biotium
Kits for increasing immunofluorescence sensitivity using HRP-catalysed tyramide signal amplification with the choice of next-generation fluorescent CF® dyes or biotin.
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CF® Dye Azide
Supplier: Biotium
CF® Dyes with an azide group. Can be used to label or detect alkyne, BCN or cyclooctyne-containing molecules by fluorescence spectroscopy, microscopy or flow cytometry following copper-catalyzed azide-alkyne cycloaddition.
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Carbohydrate coupling resin
Supplier: G-Biosciences
Carbohydrate coupling resin is designed for the covalent immobilisation of carbohydrate containing molecules, including glycoproteins, to agarose beads. It is ideal for the coupling of polyclonal antibodies as it allows for the optimal orientation of the antibodies for affinity purification.
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Affibody® Imaging Agent, Negative Control
Supplier: Abcam
Product to be used as a negative control for ErbB 2 Affibody® Molecule Imaging Agent.
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CF™ Dye lectin conjugates
Supplier: Biotium
A lectin labelled with our superior fluorescent CF® dyes is an effective and widely used cell surface stain for yeast, fungi, and mammalian cells.
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CDI amine reactive resin
Supplier: G-Biosciences
CDI amine reactive agarose consists of 6% cross-linked agarose activated with CDI (1,1’-carbonyl diimidazole) to form reactive imidazole carbamates.
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Annexin V Near IR CF® dye conjugates
Supplier: Biotium
Near-IR Annexin V fluorescent conjugates can be used to detect apoptotic cells and is suitable for in vivo imaging.