Systémy pro spojování a značení proteinů
Systémy pro navázání a označování proteinů poskytují pohodlný způsob připojení fluorescenčních značek nebo biotinů k protilátkám a větším proteinům. Tyto sady jsou ideální pro použití v mnoha aplikacích, včetně průtokové cytometrie, fluorescenční mikroskopie, imunohistochemie, primární detekce, ELISA, imunocytochemie a FISH. Tyto systémy jsou dostupné s různými barvami barviv, biotinem, fluorescenčními činidly a všemi komponenty potřebnými k provedení více konjugací a purifikací. Označování proteinů se řídí jednoduchým protokolem: reakce, separace a následné použití.
Biotin kits, HOOK™
Supplier: G-Biosciences
HOOK™ Biotin Reagent that conjugates to proteins through amines, sulfhydryls, carboxyls or carbohydrates. The amine and sulfhydryl coupling HOOK™ Biotin Reagents couple directly to the protein through their reactive groups, however the carboxyl coupling HOOK™ Biotin Reagents require a carbodiimide crosslinker and the carbohydrate coupling HOOK™ Biotin Reagents require carbohydrate oxidation before coupling.
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Peptide coupling kits, HOOK™
Supplier: G-Biosciences
The HOOK™ Peptide Coupling kits allow researchers to attach peptides to a carrier protein of choice using either primary amines or sulfhydryls in their peptide. The HOOK™ Peptide Coupling kits can be supplied with or without carrier proteins.
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FluorSave™ reagent, Calbiochem®
Supplier: Merck Millipore (Calbiochem)
FluorSave™ reagent is an aqueous mounting medium for use in conjunction with fluorescent tracers such as fluorescein, rhodamine and phycobiliprotein probes.
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Immunostainer enhancer, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Immunostain Enhancer is compatible with fluorescent and chromogenic detection and routinely increases both signal intensity and detection sensitivity.
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VeriFluor™ Far-Red Passive Reference Dye
Supplier: Biotium
A far-red passive reference dye for qPCR that frees up the ROX channel for use with JUN™ probes, EvaRuby™ qPCR dye, and other orange dyes.
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Biotin NTA
Supplier: Biotium
Biotinylated probes for detection of His-tagged proteins immobilised on nitrocellulose membranes.
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Amersham™ CyDye™ Value Packs (Bis-Reactive NHS Esters)
Supplier: Cytiva
Amersham™ CyDye™ fluors combine exceptional photostability with bright and intense signals, resulting in high sensitivity and a highly reproducible performance at a biologically suitable pH range of 3 to 10.
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Reactive dye 5-packs, Amersham CyDye™
Supplier: Cytiva
Mono-reactive dye pack contains derivatised CyDye™ that carries only one reactive group on each dye molecule for accurate labelling of amine groups, for example on antibodies or oligonucleotides.
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Amersham™ CyDye™ Value Packs (Mono-Reactive Hydrazide), Cytiva
Supplier: Cytiva
Hydrazide esters provide a convenient method for the labelling of free carbonyl groups of glycoproteins and carbohydrates. For maximum convenience, stable active hydrazide esters are supplied and stored as solid material.
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Amersham Cy3 a Cy5 Maleimid Mono-Reactive Dye 5-packs, Cytiva
Supplier: Cytiva
Mono-functional maleimides are particularly suitable for the selective labelling of molecules containing free sulfhydryl groups, such as cysteine residues in proteins and peptides and oligonucleotides.
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Fluorescent dyes, M series, streptavidin conjugate
Supplier: Abnova
Fluorescent dye, M series, products are designed to be maximally excited by one of the major light sources equipped in flow cytometers. In comparison with phycobiliprotein tandems, Fluorescent dye, M series, possesses better photostability, higher conjugation yields, and little pH sensitivity making it an ideal choice in fluorescence imaging applications.
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Protein labelling Kit, Amersham™ QuickStain
Supplier: Cytiva
The Amersham™ QuickStain Kit contains a Cy™5 fluorophore and labeling buffer for easy detection of proteins in SDSPAGE gels and on Western blotting membranes. The ready to use Cy5 N-hydroxysuccinimide (NHS) ester and Tris labeling buffer ensure robust and consistent labeling for detection of proteins in diverse samples.
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Amersham™ CyDye™ Fluorescent Nucleotides
Supplier: Cytiva
Fluorescent nucleotides for labelling DNA and RNA probes, especially during multi-colour analysis in microarrays, FISH, chromosome identification, whole chromosome painting, karyotyping, and gene mapping.
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Mono-Reactive NHS Ester dye packs, Amersham CyDye™
Supplier: Cytiva
Mono-reactive dye pack contains derivatised CyDye™ that carries only one reactive group on each dye molecule for targeted and specific labelling of amine groups, for example on antibodies, peptides or oligonucleotides.
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Amersham CyDye DIGE fluors
Supplier: Cytiva
CyDye™ DIGE fluors are available as minimal and saturation labeling dyes. The minimal dyes are intended for general 2-D application use where sufficient amounts of sample are available. The saturation dyes from the Scarce Sample Labeling Kit are designed to be used for applications where only small amounts of sample are available, for example in Laser Capture Microdissection.
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Monofunctional maleimide fluors, CyDye™
Supplier: Cytiva
Cy3 and Cy5 monofunctional maleimides are particularly suitable for the selective labelling of molecules containing free sulfhydryl groups, such as cysteine residues in proteins and peptides and oligonucleotides.
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