Hledali jste: Systémy pro extrakci proteinů
Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.
Detergent removal spin column kit, HiPPR™
Supplier: Thermo Fisher Scientific
The HiPPR™ Detergent Removal Spin Column Kit improves mass spectrometry results by efficiently removing detergents from 25 to 200 µl samples with low protein or peptide concentrations.
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Immobilised papain, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Immobilised Papain consists of a cysteine-endopeptidase that has been immobilised onto beaded agarose resin to enable generation and purification of Fab fragments from antibodies.
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Pierce™ Detergent Removal Spin Columns and Resin
Supplier: Thermo Fisher Scientific
Pierce™ Detergent removal resin enables removal of commonly used ionic, non-ionic and zwitterionic detergents from protein/peptide digest solutions.
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Detergent solutions, Surfact-Amps™
Supplier: Thermo Fisher Scientific
Surfact-Amps™ detergents are ready-to-use, diluted solutions of purified detergent that can be used in routine and high-demand protein research methods and molecular biology techniques. These detergents are useful as a component of cell lysis buffers or other solutions intended to extract and solubilise proteins, improve chromatography, prepare yeast spheroplasts, and reduce background in ELISA or Western blot methods.
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Trypsin protease, MS grade
Supplier: Thermo Fisher Scientific
Pierce™ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.
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Pierce™, Detoxi-Gel™ Endotoxin Removing Columns and Gel
Supplier: Thermo Fisher Scientific
Detoxi-Gel™ endotoxin removing columns and gel uses polymixin B ligand immobilised on beaded affinity resin to bind and extract endotoxins from antibody or protein samples.
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Mitochondria Isolation Kit for Tissue
Supplier: Thermo Fisher Scientific
The Mitochondria Isolation Kit for Tissue enables isolation of intact mitochondria from soft and hard tissue samples in about an hour.
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FOCUS™ Protein Alkylation Kit
Supplier: G-Biosciences
The FOCUS™ Protein Alkylation Kit is a proteomic grade iodoacetamide reagent kit for blocking thiols by alkylation. The kit is supplied with a proprietary buffer necessary for efficient alkylation of thiols, while minimising reoxidation of the competing thiol pairs in protein samples. Simply add an appropriate amount to reagent solutions for alkylation of protein thiol groups.
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Phosphoprotein enrichment kit, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Phosphoprotein Enrichment Kit is designed for the efficient enrichment of phosphorylated proteins derived from mammalian cells and tissues.
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Carrier proteins, maleimide-activated carrier proteins, Imject™ Pierce™
Supplier: Thermo Fisher Scientific
Imject® Maleimide-activated mcKLH and PEGylated mcKLH enable conjugation of sulfhydryl-containing peptide haptens to elicit an immune response and antibody production against the hapten.
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Carrier proteins, Imject™ Blue Carrier™
Supplier: Thermo Fisher Scientific
Imject™ Blue Carrier™ protein is a highly soluble, mollusc-derived hemocyanin that enable simple preparation of effective immunogens with amine- or carboxyl-peptide antigens.
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Total protein extraction kit, TPE™
Supplier: G-Biosciences
Total Protein Extraction (TPE™) Kit is a universal lysis system for the solubilisation of total proteins from animal, plant, yeast, bacteria, and other biological samples. The kit contains ready to use buffers for extracting total protein from cells and tissues for SDS-PAGE analysis.
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High pH reversed-phase kit, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ High pH Reversed-Phase Kit increases protein identification from LC/MS analysis through orthogonal peptide fractionation of complex peptide samples.
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Membrane protein extraction kit, Pierce™ Mem-PER™ Plus
Supplier: Thermo Fisher Scientific
The Mem-PER™ Plus Membrane Protein Extraction Kit enables small-scale solubilisation and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure. It effectively isolates membrane proteins from cultured cells and tissues using a simple benchtop microcentrifuge procedure.
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Ubiquitin enrichment kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Ubiquitin enrichment kit facilitates the isolation of polyubiquitin protein conjugates from cultured cells and tissue samples.
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FOCUS™ protein reduction-alkylation kit
Supplier: G-Biosciences
The FOCUS™ Reduction-Alkylation Kit offers a simple two-step method for reduction and alkylation of protein samples for 2D gel analysis.
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FOCUS™ protein reductant kit
Supplier: G-Biosciences
FOCUS™ Protein Reductant Kit is a water soluble, odorless, non-toxic and stable TCEP [Tris (2-carboxyethyl) phosphine] reductant for protein reduction.
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Protein fractionation kit, nuclear and cytoplasmic extraction kit
Supplier: G-Biosciences
The Nuclear and Cytoplasmic Extraction kit is for the enrichment of cytoplasmic and nuclear fractions from cultured cells and tissues. The kit generates a cleaner separation of cytoplasmic proteins from nuclear proteins and is ideal for expression and transport studies.
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Protein fractionation kit, FOCUS™ cytoplasmic and nuclear proteins
Supplier: G-Biosciences
FOCUS™ Cytoplasmic and Nuclear Protein Fractionation Kit for the fractionation of nuclear and cytoplasmic proteins from cells and tissues.
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Glycoprotein Isolation Kit, Cona And WGA, Pierce™
Supplier: Thermo Fisher Scientific
The glycoprotein isolation kit allows isolation of glycoproteins from complex mixtures, including serum, tissue and cultured cell lysates. The procedure uses concanavilin A (ConA) or wheat germ agglutinin (WGA) as a ligand to bind a specific class of glycoprotein from complex protein samples - those containing alpha-lined mannose or terminal glucose residues (in case of ConA) and N-Acetyl glucosamine (GlcNAc) groups or sialic acid (in case of WGA). This kit has been used to isolate glycoproteins from serum as well as HeLa and CHO cell lysates in approximately 50 minutes.
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Albumin depletion kit, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Albumin Depletion Kit improves serum component analysis by rapidly removing abundant albumin protein from serum samples. The albumin depletion resin provided in the kit is a high capacity, immobilised Cibacron Blue dye agarose resin, which binds a variety of species-specific albumins.
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Pierce™ In-solution Tryptic Digestion and Guanidination Kit
Supplier: Thermo Fisher Scientific
The In-Solution Tryptic Digestion and Guanidination Kit contains proteomics grade trypsin, buffers and reagents to alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry.
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In-Gel tryptic digestion kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ In-Gel Tryptic Digestion Kit provides a complete set of reagents for digesting and recovering peptide fragments from approximately 150 stained protein bands excised from SDS-PAGE (polyacrylamide gel plugs).
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Albumin/IgG removal kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Albumin/IgG Removal Kit is optimised to decrease the abundant albumin and antibody components of human serum samples in preparation for 2D electrophoresis and other protein profiling methods. By eliminating most of these two proteins, which account for nearly 70% of total serum proteins, other less abundant proteins of interest can be more easily detected and studied.
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InGel™ Array kit for protein digestion
Supplier: G-Biosciences
InGel™ Array is a 96-well format kit suitable for processing larger numbers of protein spots concurrently and is compatible with spot-picking instruments. Fewer than 96 samples a batch may also be processed without any waste.
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Cell surface protein isolation kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Cell Surface Protein Isolation Kit is a complete set of reagents for selective biotinylation and subsequent purification of mammalian cell surface proteins to the exclusion of intracellular proteins.
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Isobaric mass tag labelling kit and reagent sets, TMT10plex™
Supplier: Thermo Fisher Scientific
TMT10plex™ Isobaric Mass Tag Labelling Kit and Reagent Sets enable multiplex relative quantitation by mass spectrometry (MS).
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Mitochondria isolation kit for cultured cells
Supplier: Thermo Fisher Scientific
The Mitochondria isolation Kit for cultured cells provides a versatile, microcentrifuge-tube method for fractionating intact mitochondria from cultured mammalian cell samples in about 40 minutes.
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Organelle Enrichment Kits
Supplier: Thermo Fisher Scientific
Organelle enrichment kit for lysosomes enables enrichment of intact organelles from cells and tissue. The kit provides the media and reagents necessary to accomplish subcellular fractionation using high-speed density gradient centrifugation.
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Carrier proteins, Imject™ Maleimide-Activated Blue Carrier™
Supplier: Thermo Fisher Scientific
Imject™ Maleimide-Activated Blue Carrier™ Protein enables conjugation of a sulfhydryl-containing hapten to elicit an immune response and antibody production against the hapten. Blue Carrier™ Protein is a haemocyanin from the Concholepas concholepas mollusc with immunogenic properties similar to keyhole limpet haemocyanin. Blue Carrier™ Protein has a large mass (~8 × 106 Da) and has been successfully used in polyclonal and monoclonal antibody production for
synthetic haptens and peptides.