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Supplier: AAT BIOQUEST

A citrate buffer is an aqueous solution composed of a weak acid (citric acid) and its conjugate base (sodium citrate).

Supplier: PanReac AppliChem

These buffer solutions are useful for the calibration of high precision laboratory pH meters.

Supplier: PanReac AppliChem

pH Buffer solution, Hodnota pH: 7,00, Referenční teplota: 20 °C

Supplier: G-Biosciences

Yeast PE LB™ kit has been developed for extraction of biologically active, soluble proteins from yeast cells. Yeast PE LB™ is a proprietary improvement on the lyticase (Zymolyase®) based spheroplast preparation and extraction of soluble proteins from yeast cells. Yeast PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins.

Supplier: Thermo Fisher Scientific

Blocker™ BLOTTO is a ready-to-use 5% solution of non fat powdered milk in Tris-buffered saline for Western blot, ELISA, IHC and other detection methods.

Supplier: G-Biosciences

TE Buffer (Tris-EDTA Buffer) is a common molecular biology buffer that is used to protect DNA and RNA from degradation. The Tris buffering agent and EDTA metal chelating properties help protect DNA and RNA.

Supplier: G-Biosciences

FOCUS™ Extraction Buffers are experimentally optimised concentrations of critical agents, buffering and stabilising agents, including urea, thiourea, Nonidet® P-40, CHAPS, and sulphobetaines (SB). They are designed to produce optimal protein extraction and improved spot resolution for 2D gel analysis.

Supplier: Thermo Fisher Scientific

The NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The nuclear protein extraction method involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilise and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. The isolated proteins can be used to perform immunoassays as well as protein interaction studies such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.

Supplier: AAT BIOQUEST

ReadiUse™ Universal Kinase Reaction Stopping Buffer is an aqueous solution that effectively chelates divalent metal ions.

Supplier: OMEGA BIO-TEK

BL buffer, Omega Bio-Tek

Supplier: Thermo Fisher Scientific

The M-PER Mammalian Protein Extraction Reagent is designed to provide highly efficient total protein extraction from cultured mammalian cells.

Supplier: PanReac AppliChem

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

Supplier: Rockland Immunochemicals

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Biotium

Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)

Supplier: Rockland Immunochemicals

SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearise proteins and to impart a negative charge to linearised proteins.

Supplier: Thermo Fisher Scientific

BupH packs are pre-blended and pre-measured dry mixtures of buffers that are easy to prepare and used in protein purification and common laboratory techniques.

Supplier: G-Biosciences

BLOK™ Casein is a purified protein, used for blocking free binding sites in Western blot, ELISA plate well, immunohistochemistry and other immunochemical applications.

Supplier: Thermo Fisher Scientific

These wash buffers are designed for use in dialysis, cross-linking, enzyme assays, ELISAs, immunohistochemistry, protein plate-coating, biotinylation applications.

Supplier: Thermo Fisher Scientific

North2South™ hybridisation buffer is an aqueous buffer which is used with the North2South™ chemiluminescent detection kit.

Supplier: HyClone products (Cytiva)

Balanced salt solution used for multiple cell culture applications (powder form).

Supplier: PanReac AppliChem

Cloning Buffer, TE buffer solution (TRIS-EDTA buffer) pH 8,0

Supplier: PanReac AppliChem

Cationic detergent cetyltrimethylammonium bromide (CTAB) is used to liberate and complex with total cellular nucleic acids.

Supplier: PanReac AppliChem

HEPES buffer solution pH 8 (1 mol/l)

Supplier: Thermo Fisher Scientific

The RIPA lysis and extraction buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent for cultured mammalian cells.

Supplier: AAT BIOQUEST

ReadiCleave™ AML Cleavage Buffer provides an optimised formula to effectively remove the fluorescence tags from ReadiCleave™ AML-labeled biological targets when needed.

Supplier: 3M Food Safety

The 3M™ Mini Flip-Top Vial with 9 ml Letheen Broth is a leak-resistant plastic cylinder with a flip-open lid that seals securely for safe shipping and handling.

Supplier: G-Biosciences

FOCUS™ protein solubilisation buffer is a dry, urea-based buffer for protein solubilisation. It is a pre-mixed formulation of urea, thiourea, CHAPS and non detergent sulphobetaine for maximum solubilising strength.

Supplier: Hach

For calibration of pH meters.

Supplier: OMEGA BIO-TEK

RBB Buffer, 1000 ml, Omega Bio-Tek

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce luciferase cell lysis buffer effectively lyses adherent cells or pelleted suspension cells for analysis of promoter expression in luciferase assays.