Supplier: G-Biosciences

Tris is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane, with the formula (HOCH2)3CNH2. Tris is extensively used in as a major component in buffer solutions.

Supplier: PanReac AppliChem

These buffer solutions are useful for the calibration of high precision laboratory pH meters.

Supplier: PanReac AppliChem

Buffer solutions contain citric acid 1 H₂O (11,768 g); sodium hydroxide 50% (6 ml); hydrochlorid acid 35% (3,96 ml); preservative 1% (5 ml) and water (1 l).

Supplier: PanReac AppliChem

HEPES buffer solution pH 7,5 (1 mol/l)

Supplier: PanReac AppliChem

10X concentrated solution of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂, pH 8,3 ±0,2.

Supplier: Rockland Immunochemicals

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: VWR Chemicals

TAE is an extensively used buffer for agarose gel electrophoresis applications requiring high resolution and separation of high molecular weight, double-stranded DNA. TAE buffer is more compatible with in-gel manipulations and band recovery procedures than TBE buffer.

Supplier: LIOFILCHEM

Buffered NaCl Peptone Solution pH 7,0 is used for dissolving, suspending and diluting test samples. Its composition conforms to recommendations in the Harmonised Pharmacopoeias for the microbiological examination of non-sterile products.

Supplier: HyClone products (Cytiva)

Balanced salt solutions for cell culture applications.

Supplier: VWR Chemicals

Useful for high resolution of proteins on neutral pH SDS-PAGE gels.

Supplier: VWR Chemicals

Running buffers for both 1-D and 2-D protein gel electrophoresis.

Supplier: Hach

pH buffer standard solution with certificate of analysis, 125 ml, Hodnota pH: 4,01, Plastová láhev

Supplier: Hach

Buffers traceable to IUPAC/NIST pH scale.

Supplier: G-Biosciences

The IBS™ buffer is specifically developed for solubilisation of inclusion bodies.

Supplier: AAT BIOQUEST

Current demands for RT-PCR testing of SARS-CoV-2 has led to a worldwide shortage of RNA extraction kits.

Supplier: G-Biosciences

Tissue PE LB™ has been developed for extraction of total biologically active, soluble proteins from animal tissues. Tissue PE LB™ is based on an organic buffer and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Tissue PE LB™. Tissue PE LB™ has been tested for use with a wide variety of fresh and frozen animal tissues.

Supplier: G-Biosciences

Bacterial PE LB™ kit has been developed for the extraction of biologically active soluble proteins, including recombinant proteins and inclusion bodies, from bacterial cells. Bacterial PE LB™ is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA and RNA) released during cell lysis. The Bacterial PE LB™ lysis system eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.

Supplier: G-Biosciences

Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.

Supplier: Thermo Fisher Scientific

Phenol-chloroform, is phenol supplied premixed with chloroform:isoamyl alcohol (25:24:1) and accompanying bottle of buffer to equilibrate at either pH 6,7 or pH 8,0.

Supplier: Thermo Fisher Scientific

This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.

Supplier: Biowest

These irrigating buffers and salt solutions are sterile, physiological balanced solutions intended for use in the maintenance of mammalian cells, where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. Applications include the intermediate steps for cultivation of cells, like washing, centrifugation, suspending and counting, as well as for many analytical methods or biochemical treatments. Principally these solutions should be used to keep the cells in suspension only for minutes or a few hours maximum. These buffers and solutions are not cell culture media.

Supplier: VWR Chemicals

137 mM sodium chloride, 2,7 mM potassium chloride, 12 mM phosphate buffer

Supplier: VWR Chemicals

Cell culture buffer, TRIS buffer solution pH 7,4 (0,1 mol/l)

Supplier: HyClone products (Cytiva)

Maintain physiological pH in cell culture, despite changes in carbon dioxide concentration (produced by cellular respiration) with Cytiva HyClone HEPES solution.

Supplier: Enzo Life Sciences

Sterile and endotoxin-free PBS for use with TLRgrade™ or endotoxin-free grade reagents.

Supplier: G-Biosciences

There are many electrophoresis running buffers including: TAE running buffer (50×), TBE running buffer (10×), TBE-urea sample buffer, MOPS running buffer (10×), and MOPS/ SDS running buffer (20×). These include the SDS-PAGE sample loading buffer, SDS-PAGE running buffer, native sample loading buffer, tris glycine native gel running buffer, tricine sample buffer, tris-tricine, tris-tricine-SDS, MES SDS running buffer, MOPS/ SDS running buffer, IEF anode buffer, IEF cathode buffer, zymogram sample buffer, zymogram renature buffer, zymogram development buffer and the discontinuous buffer system.

Supplier: Thermo Fisher Scientific

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Thermo Fisher Scientific

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: G-Biosciences

Add an appropriate volume of the supplied rehydration buffer to the dry, ready-to-use, urea based equilibration buffer.

Supplier: Biotium

For simple, optimised tyramide amplification of immunostaining in cells or tissue sections. No need to add hydrogen peroxide before use.