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Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
VWR®, Tris-borate-EDTA (TBE) buffer
Supplier: VWR Chemicals
TBE is optimised for DNA electrophoresis techniques using both acrylamide and agarose. Buffers are available as liquid concentrate or Ready-Pack™ (a foil pouch containing sufficient material to prepare 1 litre of 10X concentrate).
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VWR® Buffer, MOPS-SDS, 20X, UltraPure Grade
Supplier: VWR Chemicals
Useful for high resolution of proteins on neutral pH SDS-PAGE gels.
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PBS tablets
Supplier: PanReac AppliChem
Tablets for preparation of 100 ml phosphate buffered saline (PBS).
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Tween-Tris-buffered saline (TTBS), 10X concentrate, pH 7,5
Supplier: Rockland Immunochemicals
Tween Tris buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA.
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6X GelRed prestain loading buffer with tracking dye
Supplier: Biotium
6X GelRed prestain loading buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr).
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In-situ hybridisation buffer (1.25X concentrate), Enzo Life Sciences
Supplier: Enzo Life Sciences
In Situ Hybridization Buffer provides a high quality hybridization medium for in situ hybridization analyses using either DNA or oligonucleotide probes labeled with biotin-, digoxigenin- or fluorescein-modified nucleotides.
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Buffer, TBS solution 10X concentrate (TRIS buffered saline)
Supplier: G-Biosciences
The TBS solution is primarily used as a wash and blocking buffer diluent for Western blot, ELISA and immunoassays.
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Buffer, TBS solution 10X concentrate (TRIS buffered saline) with phenol red, pH 7,4
Supplier: Cayman Chemical
250 mM Tris-base, pH 7,4, 1,37 M sodium chloride, containing 27 mM KCl, 0,4 mM phenol red.
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WB blocking buffer kit, TrueBlack®
Supplier: Biotium
The TrueBlack® WB blocking buffer kit is a ready-to-use buffer system that provides optimal specificity, sensitivity, and background signal suppression for fluorescence-based western blotting (WB).
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Protein blocking agent, BLOT-QuickBlocker™
Supplier: G-Biosciences
BLOT-QuickBlocker™ is a modified milk protein that is highly soluble and does not inhibit peroxidase detection. The modified milk protein has high blocking efficiency with a clear background.
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SSPE Buffer, 20X concentrate, pH 7,4
Supplier: Rockland Immunochemicals
20X SSPE buffer (3,0 M sodium chloride, 0,2 M sodium hydrogen phosphate, 0,02 M EDTA, pH 7,4) - DEPC treated.
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Protein blocking buffers, Superior™
Supplier: G-Biosciences
Blocking buffer is an enhanced blocking agent available in multiple formats. Superior™ Blocking Buffer contains a proprietary antigenically non-determinant protein for blocking Western blot membranes, blocking and storage of ELISA plates, and blocking prior to immunohistochemistry staining.
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Buffer, SSC solution 20X concentrate (saline-sodium citrate)
Supplier: G-Biosciences
Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)
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Protein Transfer buffers
Supplier: G-Biosciences
Efficient™ Western Transfer Buffer (20X) achieves increased protein transfer efficiency. This ready-to-use transfer buffer is prepared for optimal conductivity and efficient protein transfer, without generating excessive heat or transfer distortion.
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Immunoassay Blocking (BSA Free)
Supplier: Abcam
Immunoassay Blocking (BSA Free) (ab171535) maintains the activity of antibodies and other biomolecules adsorbed or immobilized to solid substrates.
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In-situ hybridisation buffer for HPV probes
Supplier: Enzo Life Sciences
In situ hybridisation buffer for HPV probes (ready-to use) provides a high quality hybridisation medium for In situ hybridisation analyses using either DNA or oligonucleotide probes labeled with biotin-, or fluorescein-modified nucleotides.
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Background suppressor system (Permeabilising), TrueBlack® IF
Supplier: Biotium
A buffer set that blocks non-specific antibody binding as well as direct interaction of fluorescent dyes on antibodies with cells or tissue sections.
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Proteometer-L Fluorescent Reagent
Supplier: NOVILYTIC
Set of 7 reagent vials for use with the Proteometer-L kit.
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Tween-20 solution (10%)
Supplier: Enzo Life Sciences
This Tween®-20 solution (10%) is specially designed to create the Blocking Buffer and Assay Buffer in ImmunoSet products. Each bottle is sufficient for use with 5×96 wells microtiter plates if following the instructions in our ImmunoSet protocol.
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IHC PBS tablets
Supplier: Enzo Life Sciences
Solid. 100 ml PBS (1X) tablets containing 137 mM sodium chloride, 2.7 mM potassium chloride, and 10 mM phosphate buffer.
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Proteometer-CV Consumables Kit (240)
Supplier: NOVILYTIC
Kit is for when customers needs more consumables but not the MD Unit or reactor.
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VWR®, MOPS 10X buffer for biotechnology
Supplier: VWR Chemicals
MOPS is a zwitterionic buffer used as a running buffer for denaturing agarose gel electrophoresis of RNA. Having a pH buffering range from 6,5 to 7,9, MOPS works exceptionally well with formaldehyde gels at 20 mM concentration.
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VWR® TISAB II Solution (Total Ionic Strength Adjustment Buffer) for Fluoride Determinations
Supplier: VWR Chemicals
Total ionic strength adjustment buffer solution for use with selective ion electrodes.
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VWR® UltraPure, Buffer, PBS solution (phosphate buffered saline)
Supplier: VWR Chemicals
Dulbecco's formulation without calcium and magnesium.
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Buffers, potassium phosphate buffer (0,5 mol/L) solutions
Supplier: Cayman Chemical
Potassium phosphate buffer solution (0,5 mol/l), Hodnota pH: 8,0
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Proteometer-UFT Starter Kit
Supplier: NOVILYTIC
Ulta-fast Titer kit provides 300 assays each in under 90 seconds. For human mAbs, multi-specifics, recombinant proteins.
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Diluent buffer, 10X concentrate, for dab membrane substrate
Supplier: Rockland Immunochemicals
Concentrate diluent buffer for DAB membrane substrate, 10X
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Proteasome assay buffer
Supplier: Enzo Life Sciences
Assay buffer from the 20S Proteasome Assay Kit for Drug Discovery.
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Cathepsin S Assay Buffer
Supplier: Enzo Life Sciences
50mM potassium phosphate buffer, pH 6,5, 50 mM NaCl, 2 mM EDTA, 0,01% Triton X-100®, 0,5 mM DTT.
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Proteometer-CV Full Kit Reagent (300)
Supplier: NOVILYTIC
Fluorescent reagent portion of the Proteometer-CV full kit. This is sold individually due to shipping and storage conditions.