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Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Blocking buffers, FirstChoice™
Supplier: G-Biosciences
FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non specific binding sites in ELISA, blotting, immunohistochemistry and other applications. Buffers are conveniently supplied in widely used TBS (Tris-buffered saline at pH 7,5) and PBS (phosphate-buffered saline at pH 7,5) buffers as well as in separate formulations containing Tween® 20 for improving blocking efficiencies.
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2X SDS-PAGE sample buffer without DTT or b-ME
Supplier: Rockland Immunochemicals
Sample buffer for protein electrophoresis.
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Proteasome assay buffer
Supplier: ENZO LIFE SCIENCES
Assay buffer from the 20S Proteasome Assay Kit for Drug Discovery.
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Buffers, potassium phosphate buffer (0,5 mol/L) solutions
Supplier: Cayman Chemical
Potassium phosphate buffer solution (0,5 mol/l), Hodnota pH: 6,0
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Cathepsin S Assay Buffer
Supplier: ENZO LIFE SCIENCES
50mM potassium phosphate buffer, pH 6,5, 50 mM NaCl, 2 mM EDTA, 0,01% Triton X-100®, 0,5 mM DTT.
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Diluent buffer, 10X concentrate, for dab membrane substrate
Supplier: Rockland Immunochemicals
Concentrate diluent buffer for DAB membrane substrate, 10X
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VWR® UltraPure, Buffer, PBS solution (phosphate buffered saline)
Supplier: VWR Chemicals
Dulbecco's formulation without calcium and magnesium.
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VWR® TISAB II Solution (Total Ionic Strength Adjustment Buffer) for Fluoride Determinations
Supplier: VWR Chemicals
Total ionic strength adjustment buffer solution for use with selective ion electrodes.
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Wash buffer concentrate
Supplier: ENZO LIFE SCIENCES
Liquid. TRIS buffered saline containing detergents.
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VWR® TBE Buffer Solution, 10X Concentrate (Tris-Borate-EDTA Buffer), UltraPure Grade
Supplier: VWR Chemicals
Running Buffer, TBE buffer solution 10X concentrate (Tris-borate-EDTA buffer)
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Antibody diluent (HAMA blocker) for ELISA
Supplier: Abcam
Antibody diluent (HAMA blocker) for ELISA is a proprietary reagent for immunoassays involving a sandwich antibody complex that are susceptible to heterophilic antibody interference.
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HDAC assay buffer II
Supplier: ENZO LIFE SCIENCES
50 mM Tris/Cl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1 mg/ml BSA.
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VWR®, Phosphate Buffered Saline (PBS), pH 7,4
Supplier: VWR Chemicals
Phosphate Buffered Saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate and potassium chloride. PBS helps to maintain a constant pH, is isotonic and non toxic to cells, and provide cells with water and essential inorganic ions. This formulation does not contain calcium and magnesium.
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Tween-20 solution (10%)
Supplier: ENZO LIFE SCIENCES
This Tween®-20 solution (10%) is specially designed to create the Blocking Buffer and Assay Buffer in ImmunoSet products. Each bottle is sufficient for use with 5×96 wells microtiter plates if following the instructions in our ImmunoSet protocol.
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Background suppressor system (Permeabilising), TrueBlack® IF
Supplier: Biotium
A buffer set that blocks non-specific antibody binding as well as direct interaction of fluorescent dyes on antibodies with cells or tissue sections.
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IHC PBS tablets
Supplier: ENZO LIFE SCIENCES
Solid. 100 ml PBS (1X) tablets containing 137 mM sodium chloride, 2.7 mM potassium chloride, and 10 mM phosphate buffer.
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VWR® UltraPure, Phosphate buffered saline (PBS), powdered
Supplier: VWR Chemicals
PBS, Ultra Pure Grade is a powdered, magnesium- and calcium-free formulation of PBS suitable for cell culture and for use in flow cytometry applications.
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VWR®, Tris-borate-EDTA (TBE) buffer
Supplier: VWR Chemicals
TBE is optimised for DNA electrophoresis techniques using both acrylamide and agarose. Buffers are available as liquid concentrate or Ready-Pack™ (a foil pouch containing sufficient material to prepare 1 litre of 10X concentrate).
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VWR® Buffer, MOPS-SDS, 20X, UltraPure Grade
Supplier: VWR Chemicals
Useful for high resolution of proteins on neutral pH SDS-PAGE gels.
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Dulbecco's phosphate buffered saline (DPBS), for cell culture, HyClone™
Supplier: HyClone products (Cytiva)
Balanced salt solution used for multiple cell culture applications, (liquid form).
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Wash buffer salts, lyophilised, Enzo Life Sciences
Supplier: ENZO LIFE SCIENCES
Wash Buffer Salts can be used in tissue preparations, immunohistochemical staining procedures and in assays that require HRP-linked detection reagents.
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VWR®, Cell culture salt solutions, Dulbecco's Phosphate Buffered Saline (DPBS)
Supplier: VWR Chemicals
DPBS is commonly used salt solution in cell enumeration as a diluent, for rinsing cells and as a buffer in many chromatographic procedures. DPBS is also used in FACS (Fluorescence-Activated Cell Sorting) process to wash and re-suspend cells during the dissociation process, as well as for many analytical methods or biochemical treatments.
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VWR® UltraPure, Buffer, PBS solution 10X concentrate (phosphate buffered saline), USP
Supplier: VWR Chemicals
Cell culture buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)
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VWR® UltraPure, Tris-acetate-EDTA (TAE) buffer
Supplier: VWR Chemicals
TAE is an extensively used buffer for agarose gel electrophoresis applications requiring high resolution and separation of high molecular weight, double-stranded DNA. TAE buffer is more compatible with in-gel manipulations and band recovery procedures than TBE buffer.
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VWR®, Phosphate buffered saline (PBS) 1X, for biotechnology
Supplier: VWR Chemicals
137 mM sodium chloride, 2,7 mM potassium chloride, 12 mM phosphate buffer
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VWR® Tris Buffer Solution, pH 7,4 (0,1 mol/L) for Biotechnology
Supplier: VWR Chemicals
Cell culture buffer, TRIS buffer solution pH 7,4 (0,1 mol/l)
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TPE buffer, 10X tris-phosphate-EDTA, pH 8
Supplier: Rockland Immunochemicals
Running Buffer, Tris-phosphate-EDTA 10X (TPE) (0,9 M tris-phosphate 0,01 M EDTA pH 8,0)
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VWR® UltraPure, Tris-glycine buffers, 10X concentrate
Supplier: VWR Chemicals
Running buffers for both 1-D and 2-D protein gel electrophoresis.