You searched for: Pufry

Supplier: Cayman Chemical

Formulation: Concentrated TBS (250 mM Tris-base; pH 7,4; 1,37 M sodium chloride and 27 mM KCl).

Supplier: Biotium

Fish gelatin is commonly used in blocking solutions to minimise background noise in immunodetection procedures such as Western Blotting and immunostaining. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or for blocking and dilution of antibodies during immunostaining procedures. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.

Supplier: Rockland Immunochemicals

A blocking buffer specifically designed for western blotting using fluorochrome conjugated antibodies. Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in tris buffered saline at pH 7,6 with thimerisol added as an antimicrobial agent.

Supplier: Enzo Life Sciences

Hybridisation wash reagent provides a high quality hybridisation wash solution for in situ hybridisation analyses using DNA probes labeled with biotin, digoxigenin or fluorescein.

Supplier: Rockland Immunochemicals

Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridisation process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, in situ hybridisation, or DNA microarrays). SSC is used to help control the stringency of hybridisation in these steps.

Supplier: G-Biosciences

Protein-Free™ Blocking buffer is a formulation of non-protein agents that eliminate non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications. The absence of protein from the Protein-Free™ Blocking buffer eliminates problems associated with traditional protein based blockers, such as cross-reactivity and interference from glycosylated proteins.

Supplier: VWR Chemicals

PBS Tween® 20 blot wash buffer is a 20X concentration with 1% Tween® 20.

Supplier: VWR Chemicals

Phosphate Buffered Saline (PBS) with 0,05% Tween® 20, pH 7,5 is a ready-to-use 1× concentration.

Supplier: VWR Chemicals

Each pack of powdered blocking buffer makes one liter of 1X solution.

Supplier: VWR Chemicals

Rapid transfer buffer is a simple, single component system for quick and efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications. Transfer is completed in 10 or 20 minutes using a standard semi-dry or wet transfer apparatus, respectively.

Supplier: VWR Chemicals

RapidBlock™ solution reduces blocking time to five minutes for Western and dot blotting procedures on PVDF and nitrocellulose membranes. This protein-free formulation minimises cross-reactivity and non specific antibody binding, generating blots with low background and enhanced signal-to-noise ratio.

Supplier: NOVILYTIC

Fluorescent reagent for the Proteometer-L full kit. This is sold separately due to shipping conditions. Store at −20 °C.

Supplier: Enzo Life Sciences

Permeabilization, (buffer)

Supplier: NOVILYTIC

Replacement fluorescent kit reagent for the Proteometer-CV.

Supplier: Enzo Life Sciences

MMP-3 Fluorescent assay buffer

Supplier: VWR Chemicals

Ozářený koncentrát PBS, vhodný pro použití při promývání buněk po naředění.

Supplier: VWR Chemicals

Hybridisation cocktail offering blocking and hybridisation for optimised complimentary base pairing of DNA and RNA in Southern blot, Northern blot, dot blot, colony and in situ hybridisation.

Supplier: VWR Chemicals

Irradiated solution, pH 7,2, for washing blots.

Supplier: Enzo Life Sciences

SIRT assay buffer

Supplier: Enzo Life Sciences

50 mM HEPES, pH 7,4, 100 mM NaCl, 0,1% CHAPS, 10 mM DTT, 1 mM EDTA, 10% glycerol.

Supplier: Enzo Life Sciences

PTP1B 2x assay buffer

Supplier: NOVILYTIC

Specifically formulated buffer packets for use with the Proteometer-UFT kit, sold in sets of 3.

Supplier: NOVILYTIC

Specifically formulated buffer packets for use with the Proteometer-L Kit, sold in sets of seven.

Supplier: Cayman Chemical

Sodium phosphate stock solution (0,5 mol/L), Hodnota pH: 8,0

Supplier: Enzo Life Sciences

HDAC assay buffer

Supplier: Enzo Life Sciences

MMP-1 colorimetric assay buffer

Supplier: VWR Chemicals

Phosphate Buffered Saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate and potassium chloride. PBS helps to maintain a constant pH, is isotonic and non toxic to cells, and provide cells with water and essential inorganic ions. This formulation does not contain calcium and magnesium.

Supplier: Rockland Immunochemicals

Sample buffer for protein electrophoresis.

Supplier: G-Biosciences

FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non specific binding sites in ELISA, blotting, immunohistochemistry and other applications. Buffers are conveniently supplied in widely used TBS (Tris-buffered saline at pH 7,5) and PBS (phosphate-buffered saline at pH 7,5) buffers as well as in separate formulations containing Tween® 20 for improving blocking efficiencies.

Supplier: VWR Chemicals

TBE is optimised for DNA electrophoresis techniques using both acrylamide and agarose. Buffers are available as liquid concentrate or Ready-Pack™ (a foil pouch containing sufficient material to prepare 1 litre of 10X concentrate).