Hledali jste: Pufry
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
VWR® PBS/Tween® 20, 20X, Blot Wash Buffer, UltraPure Grade
Supplier: VWR Chemicals
PBS Tween® 20 blot wash buffer is a 20X concentration with 1% Tween® 20.
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VWR®, Blocking buffer, non-fat powdered milk
Supplier: VWR Chemicals
Each pack of powdered blocking buffer makes one liter of 1X solution.
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Phosphate buffered saline (PBS), 10X
Supplier: G-Biosciences
PBS 10X solution is used as a wash buffers for Western blotting, ELISA and other applications.
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Hybridisation buffers
Supplier: G-Biosciences
The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).
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VWR® Separating Gel Buffer, 4X
Supplier: VWR Chemicals
4X concentrated buffer solution (1,5 M Tris, pH 8,8 and 0,4% SDS). Used for preparation of standard acrylamide:bis-acrylamide gels as well as preparation of gels using PAGE-PLUS™ acrylamide solution.
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VWR®, SSC buffer, 20X, ultra-pure grade
Supplier: VWR Chemicals
SSC buffer is a hybridisation buffer for Northern and Southern blots.
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SSC buffer, 20X concentrate, pH 7,0
Supplier: Rockland Immunochemicals
Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridisation process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, in situ hybridisation, or DNA microarrays). SSC is used to help control the stringency of hybridisation in these steps.
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Blocking buffers, Protein-Free™
Supplier: G-Biosciences
Protein-Free™ Blocking buffer is a formulation of non-protein agents that eliminate non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications. The absence of protein from the Protein-Free™ Blocking buffer eliminates problems associated with traditional protein based blockers, such as cross-reactivity and interference from glycosylated proteins.
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J.T.Baker®, Sheath fluid, 3200/3400
Supplier: Avantor
Sheath fluid, 3200/4000, J.T.Baker®
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Caspase assay buffer
Supplier: ENZO LIFE SCIENCES
50 mM HEPES, pH 7,4, 100 mM NaCl, 0,1% CHAPS, 10 mM DTT, 1 mM EDTA, 10% glycerol.
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PBS tablets
Supplier: PanReac AppliChem
Tablets for preparation of 100 ml phosphate buffered saline (PBS).
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6X GelRed prestain loading buffer with tracking dye
Supplier: Biotium
6X GelRed prestain loading buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr).
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Tween-Tris-buffered saline (TTBS), 10X concentrate, pH 7,5
Supplier: Rockland Immunochemicals
Tween Tris buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA.
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In-situ hybridisation buffer (1.25X concentrate), Enzo Life Sciences
Supplier: ENZO LIFE SCIENCES
In Situ Hybridization Buffer provides a high quality hybridization medium for in situ hybridization analyses using either DNA or oligonucleotide probes labeled with biotin-, digoxigenin- or fluorescein-modified nucleotides.
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Fixation/Permeabilisation, (buffer)
Supplier: ENZO LIFE SCIENCES
Fixation/Permeabilization, (buffer)
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Protein blocking buffers, Superior™
Supplier: G-Biosciences
Blocking buffer is an enhanced blocking agent available in multiple formats. Superior™ Blocking Buffer contains a proprietary antigenically non-determinant protein for blocking Western blot membranes, blocking and storage of ELISA plates, and blocking prior to immunohistochemistry staining.
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In-situ hybridisation buffer for HPV probes
Supplier: ENZO LIFE SCIENCES
In situ hybridisation buffer for HPV probes (ready-to use) provides a high quality hybridisation medium for In situ hybridisation analyses using either DNA or oligonucleotide probes labeled with biotin-, or fluorescein-modified nucleotides.
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Protein Transfer buffers
Supplier: G-Biosciences
Efficient™ Western Transfer Buffer (20X) achieves increased protein transfer efficiency. This ready-to-use transfer buffer is prepared for optimal conductivity and efficient protein transfer, without generating excessive heat or transfer distortion.
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Buffer, SSC solution 20X concentrate (saline-sodium citrate)
Supplier: G-Biosciences
Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)
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Buffers, sodium phosphate buffer solutions (0,5 mol/L)
Supplier: Cayman Chemical
Sodium phosphate stock solution (0,5 mol/L), Hodnota pH: 7,0
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MMP-1 colorimetric assay buffer
Supplier: ENZO LIFE SCIENCES
MMP-1 colorimetric assay buffer
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VWR®, MOPS 10X buffer for biotechnology
Supplier: VWR Chemicals
MOPS is a zwitterionic buffer used as a running buffer for denaturing agarose gel electrophoresis of RNA. Having a pH buffering range from 6,5 to 7,9, MOPS works exceptionally well with formaldehyde gels at 20 mM concentration.
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Buffer, TBS solution 10X concentrate (TRIS buffered saline)
Supplier: G-Biosciences
The TBS solution is primarily used as a wash and blocking buffer diluent for Western blot, ELISA and immunoassays.
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SSPE Buffer, 20X concentrate, pH 7,4
Supplier: Rockland Immunochemicals
20X SSPE buffer (3,0 M sodium chloride, 0,2 M sodium hydrogen phosphate, 0,02 M EDTA, pH 7,4) - DEPC treated.
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WB blocking buffer kit, TrueBlack®
Supplier: Biotium
The TrueBlack® WB blocking buffer kit is a ready-to-use buffer system that provides optimal specificity, sensitivity, and background signal suppression for fluorescence-based western blotting (WB).
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Protein blocking agent, BLOT-QuickBlocker™
Supplier: G-Biosciences
BLOT-QuickBlocker™ is a modified milk protein that is highly soluble and does not inhibit peroxidase detection. The modified milk protein has high blocking efficiency with a clear background.
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Buffer, TBS solution 10X concentrate (TRIS buffered saline) with phenol red, pH 7,4
Supplier: Cayman Chemical
250 mM Tris-base, pH 7,4, 1,37 M sodium chloride, containing 27 mM KCl, 0,4 mM phenol red.