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Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Phosphate buffered saline (PBS), Sigma-Aldrich®

Supplier: Merck

Contents of one pouch, when dissolved in one liter of distilled or deionised water, will yield 0,01 M phosphate buffered saline (NaCl 0,138 M; KCl - 0,0027 M); TWEEN® 20 - 0,05%, pH 7,4, at 25 °C.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Sigma-Aldrich®

Supplier: Merck

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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TISAB II Solution, Supelco®

Supplier: Merck

TISAB, in general, is mixed with samples, as it helps in maintaining the ionic strength and pH constant. It is mainly used to decomplex metal-F complexes.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Sigma-Aldrich®

Supplier: Merck

TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated, water-soluble, and protected from degradation. This concentrate can be easily diluted to 1X or 0,5X before use (with molecular biology grade water).

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Buffer solutions

Supplier: Hanna

Solutions in sachets ensure optimum precision. The plastic-coated sachet provides a perfect liquid seal and good protection from light. The solutions should only be used once to avoid any danger of contamination. They also satisfy the requirements of the GLP and ISO standards. They have been adjusted using a pH meter that has been calibrated according to the NIST standards.

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Technical pH buffer solutions in sachets, HI 50000 series

Supplier: Hanna

These technical calibration buffers produced in accordance with ISO 3696/BS3978 standards using high purity salts, deionised water, certified weight-checked balances, and Class A glassware in a temperature controlled environment monitored with certified thermometers.

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pH Buffer solutions, CX-1

Supplier: Thermo Fisher Scientific

Delivers robust reproducible gradients with the Thermo Scientific™ pH buffer platform that is applicable to a wide range of MAbs and ready to use with existing LC columns and systems, without the need to formulate mobile phases.