Pufry
Pufry odolávají změnám pH a chrání vzorky při přidání kyselin nebo zásad. Práškové a kapalné formy účinně sledují proteiny během elektroforézy, izolace a separace. Slané pufry lze také použít ke kalibraci a standardizaci pH metrů pro přesné měření. Blokovací látky zabraňují navázání protilátek na místa bez membrány, která by mohla vytvořit rušivé pozadí. Pufry mohou být k dispozici v různých koncentracích, aby vyhovovaly jakýmkoli potřebám, a to v kapalné nebo dehydratované formě.
TPE buffer, 10X tris-phosphate-EDTA, pH 8
Supplier: Rockland Immunochemicals
Running Buffer, Tris-phosphate-EDTA 10X (TPE) (0,9 M tris-phosphate 0,01 M EDTA pH 8,0)
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In situ hybridisation wash reagent
Supplier: Enzo Life Sciences
Hybridisation wash reagent provides a high quality hybridisation wash solution for in situ hybridisation analyses using DNA probes labeled with biotin, digoxigenin or fluorescein.
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Blocking buffers
Supplier: Rockland Immunochemicals
A blocking buffer specifically designed for western blotting using fluorochrome conjugated antibodies. Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in tris buffered saline at pH 7,6 with thimerisol added as an antimicrobial agent.
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Native lysis Buffer, Abcam
Supplier: Abcam
Abcam’s 1X Native lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells.
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RIPA cell lysis buffer 2
Supplier: Enzo Life Sciences
RIPA cell lysis buffer 2, Enzo Life Sciences
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100x Citrate Buffer pH 6.0, Abcam
Supplier: Abcam
Citrate buffer pH 6.0 for heat-induced antigen retrieval (HIER) during IHC (ab64236).
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Dry buffer packs, JAW™
Supplier: G-Biosciences
Premixed buffer powders that simply require adding water to generate a ready-to-use buffer.
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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)
Supplier: PanReac AppliChem
Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)
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In-situ hybridisation buffer for HPV probes
Supplier: Enzo Life Sciences
In situ hybridisation buffer for HPV probes (ready-to use) provides a high quality hybridisation medium for In situ hybridisation analyses using either DNA or oligonucleotide probes labeled with biotin-, or fluorescein-modified nucleotides.
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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)
Supplier: Rockland Immunochemicals
Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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Extraction Buffer 5X PTR, Abcam
Supplier: Abcam
Extraction buffer 5X PTR is formulated for preparing cell or tissue extracts for use in immunoassays, with added phosphatase inhibitors and protease inhibitor aprotinin. - Cited in over 20 publications - Phosphatase and protease inhibitors – contains inhibitors to preserve sample integrity - Easy to use – dilute to 1X with distilled water before use, or use with extraction enhancer buffer 50X for enhanced extraction - Compatible with a variety of sample types
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Protein blocking buffers, Superior™
Supplier: G-Biosciences
Blocking buffer is an enhanced blocking agent available in multiple formats. Superior™ Blocking Buffer contains a proprietary antigenically non-determinant protein for blocking Western blot membranes, blocking and storage of ELISA plates, and blocking prior to immunohistochemistry staining.
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Optimizer Buffer™ for optimal cross-linking and modification
Supplier: G-Biosciences
The conjugation and modification reactions used to cross-link proteins or couple labels to proteins, such as biotin, enzymes, and fluorescent dyes, require certain conditions, including pH and chemical composition for optimal conjugation. Many common buffers routinely used in laboratories have an inhibitory effect on conjugation reactions; for example, Tris buffers inhibit coupling to amines.
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G-Biosciences Sodium Chloride-Sodium Citrate Buffer (20X)
Supplier: G-Biosciences
Saline-Sodium Citrate Hybridization Buffer (20X) controls stringency during the washing steps in Southern blotting, in situ hybridization, DNA microarray or Northern blotting.



