Pufry

Pufry odolávají změnám pH a chrání vzorky při přidání kyselin nebo zásad. Práškové a kapalné formy účinně sledují proteiny během elektroforézy, izolace a separace. Slané pufry lze také použít ke kalibraci a standardizaci pH metrů pro přesné měření. Blokovací látky zabraňují navázání protilátek na místa bez membrány, která by mohla vytvořit rušivé pozadí. Pufry mohou být k dispozici v různých koncentracích, aby vyhovovaly jakýmkoli potřebám, a to v kapalné nebo dehydratované formě.

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SIRT assay buffer

Supplier: Enzo Life Sciences

SIRT assay buffer

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Buffer, TBS solution 10X concentrate (TRIS buffered saline) pH 7,4

Supplier: Cayman Chemical

Formulation: Concentrated TBS (250 mM Tris-base; pH 7,4; 1,37 M sodium chloride and 27 mM KCl).

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PBS and TBS buffers

Supplier: Rockland Immunochemicals

Phosphate buffered and Tris buffered saline are suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA. Other applications may require detergents or other additional components.

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Caspase assay buffer

Supplier: Enzo Life Sciences

50 mM HEPES, pH 7,4, 100 mM NaCl, 0,1% CHAPS, 10 mM DTT, 1 mM EDTA, 10% glycerol.

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Kinase Dilution Buffer III, Abcam

Supplier: Abcam

Solution of buffer used for diluting kinase protein.

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)

Supplier: Thermo Fisher Scientific

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

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Blocking buffer, fish gelatin

Supplier: Biotium

Fish gelatin is commonly used in blocking solutions to minimise background noise in immunodetection procedures such as Western Blotting and immunostaining. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or for blocking and dilution of antibodies during immunostaining procedures. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.

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Buffers, TRIS HCl solutions, pH range 7,2 to 8,5 (1 mol/L)

Supplier: Rockland Immunochemicals

Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids.

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TPE buffer, 10X tris-phosphate-EDTA, pH 8

Supplier: Rockland Immunochemicals

Running Buffer, Tris-phosphate-EDTA 10X (TPE) (0,9 M tris-phosphate 0,01 M EDTA pH 8,0)

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In situ hybridisation wash reagent

Supplier: Enzo Life Sciences

Hybridisation wash reagent provides a high quality hybridisation wash solution for in situ hybridisation analyses using DNA probes labeled with biotin, digoxigenin or fluorescein.

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Blocking buffers

Supplier: Rockland Immunochemicals

A blocking buffer specifically designed for western blotting using fluorochrome conjugated antibodies. Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in tris buffered saline at pH 7,6 with thimerisol added as an antimicrobial agent.

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Native lysis Buffer, Abcam

Supplier: Abcam

Abcam’s 1X Native lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells.