Pufry

Pufry odolávají změnám pH a chrání vzorky při přidání kyselin nebo zásad. Práškové a kapalné formy účinně sledují proteiny během elektroforézy, izolace a separace. Slané pufry lze také použít ke kalibraci a standardizaci pH metrů pro přesné měření. Blokovací látky zabraňují navázání protilátek na místa bez membrány, která by mohla vytvořit rušivé pozadí. Pufry mohou být k dispozici v různých koncentracích, aby vyhovovaly jakýmkoli potřebám, a to v kapalné nebo dehydratované formě.

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Lysis buffer, 0,5% Tween 20, pH 8,0, for isolation of His-tag modified proteins

Supplier: Thermo Fisher Scientific

Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins, Thermo Fisher Scientific

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Buffers, concentrated solutions, Fluka™

Supplier: Honeywell Chemicals

pH Buffer, concentrate, Hodnota pH: 5,00, ±0.02, Ampoule

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Phosphate buffer solution pH 7,00 (20 °C), Fluka™

Supplier: Honeywell Chemicals

Potassium dihydrogen phosphate / di-sodium hydrogen phosphate

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10% SDS (Sodium Dodecyl Sulfate), Corning®

Supplier: Corning

Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.

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Laemmli SDS sample buffer, reducing (4X)

Supplier: Thermo Fisher Scientific

Loading Buffer, Laemmli SDS Sample Buffer, reducing, 4X

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G-Biosciences Tris-EDTA-NaCl Buffer

Supplier: G-Biosciences

Tris-EDTA-NaCl buffer solution at pH 8,0.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Millipore®

Supplier: Merck Millipore (Calbiochem‎)

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Phosphate-buffered saline (PBS, 10X), pH 7,4, for Western blot

Supplier: Thermo Fisher Scientific

Washing buffer, Phosphate-buffered saline (PBS, 10X)

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Buffer, ACES, 0,5 M solution

Supplier: Thermo Fisher Scientific

ACES, 0,5M buffer solution, pH 7,0, Colourless, Liquid

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NP-40 lysis buffer, low salt

Supplier: Thermo Fisher Scientific

NP-40 lysis buffer, low salt, Thermo Fisher Scientific

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Stacking buffer (4X)

Supplier: Thermo Fisher Scientific

Pufr pro elektroforézu, Stacking Buffer, 4X

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Phosphate, 0,2 M buffer solution, pH 7,6

Supplier: Thermo Fisher Scientific

Phosphate buffer soln, 0,2 M, Hodnota pH: 7,6, Liquid

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Tween 20 washing buffer, 0,5% in PBS (10X)

Supplier: Thermo Fisher Scientific

Washing buffer, Tween 20 washing buffer, 0,5% in PBS (10X)

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Buffer, TBE solution 5X concentrate (TRIS-borate-EDTA buffer)

Supplier: Thermo Fisher Scientific

Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)

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CHES, 0.5 M buffer solutions

Supplier: Thermo Fisher Scientific

2-(Cyclohexylamino)ethanesulfonic acid, CHES.

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TAE buffer, 10X solution, molecular biology grade, ultrapure

Supplier: Thermo Fisher Scientific

10X TAE solution consisting of 400 mM Tris and 0,01 M EDTA in high purity dH₂O adjusted to pH 8,3. Solution is 0,2 μm filtered and dispensed into durable, square Nalgene® bottles.