Hledali jste: Nucleic Acid Purification Kits and Reagents

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Plasmid Mini Kit I is designed to isolate up to 30 µg of high-quality plasmid DNA from 1 to 5 ml bacterial cultures in 30 minutes or less. Plasmid DNA purification is simplified with HiBind® Mini Column technology into three quick steps: Bind, wash, and elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, and manual/automated fluorescent DNA sequencing. E.Z.N.A.® Plasmid Mini Kit I is available in either V-spin or Q-spin column format. V-spin column formats have an attached lid while Q-spin columns do not. Both versions can be used for centrifugation or vacuum protocols.

Supplier: Corning

The AxyPrep™ Mag PCR Normalizer Kit utilizes a paramagnetic bead-based purification system for PCR clean up.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Plasmid Maxi Kit is the maxi scale system to deliver high-quality plasmid DNA using the spin column format, without costly accessories. The HiBind® DNA Maxi column facilitates the binding, washing, and elution steps, thus enabling multiple samples to be simultaneously processed. Although yields vary according to plasmid copy number, E.coli strain, and conditions of growth, 50 to 200 ml of overnight culture in LB medium typically produces 0,5 to 1 mg of high copy number plasmid DNA. Up to 500 ml of culture may be processed when working with low copy number plasmid. The product is suitable for automated fluorescent DNA sequencing (typical reads exceed 800 bp), restriction enzyme digestion, transfection of mammalian cells, transcription invitro, and other applications.

Supplier: OMEGA BIO-TEK

Plazmid izolovaný pomocí tradičních purifikačních metod běžně obsahuje vysoké hladiny endotoxinů, které mohou významně interferovat s následnými transfekčními experimenty. U souprav E.Z.N.A.® Endo-Free Plasmid Kit je do postupu plazmidové purifikace za účelem získání plazmidu o vysoké kvalitě vhodného pro transfekci včleněn krok efektivního odstranění endotoxinů.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Bacterial RNA kit is designed for isolation of high-quality total RNA from variety of bacterial strains. Up to 1×10⁹ log-phase bacterial cells can be processed. This kit uses an improved lysis procedure to ensure the complete lysis of bacterial cells. Purified RNA is suitable for downstream applications such as RT-PCR and hybridisation techniques.

Supplier: OMEGA BIO-TEK

Purify endotoxin free (<0,1 EU/µg) plasmid DNA using magnetic beads from up to 50 ml culture volume.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Forensic DNA Kit is designed to provide a rapid and easy method for the isolation of genomic DNA from forensic samples such as dry blood, buccal swabs, and semen samples for consistent PCR and Southern analysis. This kit can also be used for the preparation of genomic DNA from mouse tail snips, whole blood, buffy coat, serum, and plasma.

Supplier: OMEGA BIO-TEK

Purifikace DNA z gelů je běžná technika používaná pro izolaci specifických fragmentů DNA. Většina metod však buďto neumožňuje kompletní odstranění agarózy (což může vést k problémům při následných aplikacích), nastříhá řetězce DNA nebo poskytuje velmi nízké výtěžky.

Supplier: Corning

Removing excess dye terminator is an essential step prior to Sanger sequencing. Carryover of the excess dye into the sequencing reactions may result in dye blobs resulting in inaccurate results. The AxyPrep™ Mag DyeClean kit utilises a paramagnetic bead-based purification system for Sanger sequencing reaction dye terminator clean-up. The protocol is simple and comprises binding, washing and elution steps which can be performed directly in the thermal cycling plate. This kit requires no centrifugation or filtration steps.

Supplier: OMEGA BIO-TEK

The Mag-Bind® Blood and Tissue DNA HDQ 96 Kit is designed for rapid and reliable isolation of high quality genomic DNA from 100 to 200 μl blood samples, buccal swabs, buffy coat and cultured cells. All heating steps that limit robotic applications have been removed to allow for faster processing. Mag-Bind® particles HDQ provide quick magnetic response time reducing overall processing time. Utilising paramagnetic particles provides high quality DNA that is suitable for direct use in most downstream applications, such as qPCR, PCR and Next Generation Sequencing. The Mag-Bind® HDQ Blood DNA and Tissue 96 Kit is compatible with many robotic liquid handlers and magnetic processors, including Thermo Scientific KingFisher® Flex.

Supplier: OMEGA BIO-TEK

The Mag-Bind® Plant DNA 96 plus kit allows rapid and reliable isolation of high-quality genomic DNA from a wide variety of difficult to lyse and high polysaccharide plant species and tissues. The system utilises a CTAB-based lysis buffer without the need for a chloroform or other organic solvent steps. The proprietary wash buffer systems eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. This kit is designed for manual or fully automated high throughput preparation of genomic, chloroplast, and mitochondrial DNA. The Mag-Bind® Plant DNA plus kit also provides greater flexibility than silica plate based systems as partial plates can be used and can be scaled up to a 24-well format with the appropriate magnetic stand. The streamlined protocol can be easily adapted on most robotic liquid handling platforms. Purified DNA is suitable for PCR, qPCR, restriction digestion, hybridisation applications, as well as more demanding applications, such as RAPD.

Supplier: OMEGA BIO-TEK

Souprava E.Z.N.A.® SP Plant DNA Kit je navržena speciálně pro rychlou a spolehlivou izolaci vysoce kvalitní celkové buněčné DNA ze širokého spektra rostlinných druhů. Touto metodou je možno do 40 minut zpracovat až 100 mg čerstvého, případně až 30 mg suchého vzorku. Takto purifikovaná DNA je vhodná pro následné aplikace, včetně PCR, restrikčního štěpení a hybridizačních technik.

Supplier: OMEGA BIO-TEK

Souprava pro izolaci genomové DNA z krve, E.Z.N.A.® SQ blood DNA kit, nabízí spolehlivou metodu izolace genomové DNA s vysokou molekulární hmotností z čerstvé, zmražené nebo celé krve ošetřené proti srážení. Metodu lze také použít k izolaci genomové DNA z buffy coatu, kostní dřeně nebo buněk z kultur. Bez extrakce fenolem či chloroformem. Časově náročné kroky, jako je ultracentrifugace CsCl gradientu, jsou eliminovány. DNA purifikovaná pomocí E.Z.N.A.® SQ blood DNA soupravy je připravena pro následné aplikace, jako jsou PCR, Southern blotting a restrikční enzymatické štěpení.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Blood RNA Kit is designed for the isolation of total intracellular RNA from up to 1 ml of fresh, or frozen whole blood treated with any common anticoagulant such as heparin, EDTA or acid-citrate-dextrose. The procedure completely removes contaminants and enzyme inhibitors making total RNA isolation fast, convenient and reliable. Red blood cells are selectively lysed and white cells are collected by centrifugation. After lysis of white blood cells under denaturing conditions that inactivate RNases, the lysate is homogenised with a homogeniser spin column. The sample is then applied to a HiBind® spin column. Cellular debris and other contaminants such as haemoglobin are effectively washed away and high-quality RNA is finally eluted in DEPC treated water.

Supplier: Corning

The efficient removal of unincorporated dNTPs, salts and enzymes after any PCR is detrimental to the success or failure of downstream applications, such as genotyping, Sanger, and Next Generation Sequencing. The AxyPrep™ Mag PCR Clean Up Kit utilises a paramagnetic bead technology for rapid high throughput purification of PCR products. Additionally, the AxyPrep™ Mag PCR Clean Up Kit employs an optimised buffer to selectively bind PCR amplicons 60 bp and larger to paramagnetic beads, providing end users flexibility and high recovery options. The protocol comprises binding, washing and eluting steps, where primers, nucleotides, salts and enzymes in the reaction mixture are efficiently removed leaving a purified PCR product that is essentially free of contaminants. No centrifugation or filtration steps are required, allowing the process to be adapted to multiple commercially available automation platforms.

Supplier: OMEGA BIO-TEK

Souprava E.Z.N.A.® bacterial DNA kit umožňuje rychlou a spolehlivou izolaci vysoce kvalitní celkové buněčné DNA ze širokého spektra bakterií. Souprava využívá optimálních lyzačních podmínek, v každé kolonce lze zpracovat až 1×10⁹ bakteriálních buněk. Bez organické extrakce - úspora času i plastového odpadu, což umožňuje zpracovávat současně více vzorků. Bakteriální buňky jsou pěstovány do log-fáze a následně použity. Buněčná stěna je odstraněna štěpením lysozymem a mechanicky pomocí kuliček, následně štěpením proteázou. Po lyzi jsou upraveny vazebné podmínky a vzorek je dále purifikován pomocí HiBind DNA centrifugačních kolonek. Dva rychlé promývací kroky odstraní stopy solí a kontaminujících proteinů a poté je DNA eluována vodou nebo elučním pufrem. Purifikovaná DNA může být přímo použita pro následné aplikace bez nutnosti dalšího čištění.

Supplier: OMEGA BIO-TEK

Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free plasmid mini kit II integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<0,1 EU/µg) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding condition, the cell lysate is applied into the HiBind® DNA column and purified DNA is eluted from the column membrane.

Supplier: OMEGA BIO-TEK

Sequentially isolate DNA and RNA in two separate eluates from the same FFPE sample.

Supplier: OMEGA BIO-TEK

Isolate DNA from molluscs and insects using spin columns.

Supplier: Enzo Life Sciences

PCR/Gel Clean Up Kits are quick and simple kits for purifying DNA from PCR, gels and labelling reactions for a variety of downstream applications.

Supplier: OMEGA BIO-TEK

The Mag-Bind® Stool DNA 96 Kit allows rapid and reliable isolation of high-quality genomic DNA (both pathogen and host DNA) from stool samples.

Supplier: OMEGA BIO-TEK

Automation-ready, prefilled 96-well plates for quick and convenient DNA extraction from blood, saliva, cultured cells, and swabs for magnetic processors.

Supplier: OMEGA BIO-TEK

Hi-Bind® DNA mini columns are silica glass fibre columns that can bind up to 100 μg of genomic DNA or 35 μg of plasmid DNA per prep. Columns feature an attached lid and a vacuum luer for processing with vacuum manifolds and by centrifugation.

Supplier: Corning

The AxyPrep™ FragmentSelect-I Kit is optimized for Illumina next generation sequencing platforms and Life Technologies (SOLiD) fragment size selection needs to simplify the library construction workflow. The kit is designed for fragment size selection needs in the Roche/454’s DNA Genome sequencer library construction workflow.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® miRNA isolation kit uses a rapid procedure to isolate small RNAs, such as micro RNA (miRNA), small interfering RNA (siRNA), and small nuclear RNA (snRNA), from wide range of tissues and cells. The fast and efficient silica membrane based method isolates total RNA ranging in size from kilo based down to decamers. The kit also provides reagents and a procedure to enrich the population of RNAs that are 200 bases and smaller, which enhances the sensitivity of small RNA detection by solution hybridisation, Northern analysis, and other methods.

Supplier: OMEGA BIO-TEK

The MicroElute RNA clean-up kit is designed to purify and concentrate RNA (from 50 μg down to picogram amounts) from enzymatic reactions or for desalting RNA samples (e.g. following phenol extraction and ethanol precipitation). The procedure uses lower elution volumes, making it well suited for applications require highly concentrated RNA sample.

Supplier: OMEGA BIO-TEK

Selektivní štěpení proteázou uvolňuje z FFPE vzorků mikrogramová množství DNA. Purifikované nukleové kyseliny, ačkoliv vysoce fragmentované, jsou vhodné pro širokou škálu následných genomových analýz a analýz genové exprese. Soupravy E.Z.N.A.® obsahující centrifugační kolonky jsou ideální pro nízký počet vzorků, zatímco Mag-Bind® soupravy fungující na principu magnetických kuliček jsou navrženy speciálně pro uživatele s vysokým počtem vzorků s možností automatizace.

Supplier: OMEGA BIO-TEK

Souprava E.Z.N.A.® Water DNA Kit je navržena pro izolaci vysoce čisté buněčné DNA z vodních vzorků obsahujících huminové kyseliny a inhibitory PCR. Tato souprava je velice účinná při izolaci DNA z bakterií, u kterých se těžko provádi lyze, z hub a z řas, které se nachází ve vodě z vodovodního řádu, jezerech, řekách a v kanalizaci. DNA purifikovaná pomocí soupravy E.Z.N.A.® Water DNA Kit je připravena pro následné aplikace, jako jsou PCR, Southern blotting a anylýza SNP.

Supplier: OMEGA BIO-TEK

Isolate DNA from soil and water samples using magnetic beads.

Supplier: Quantabio

Rapid extraction and purification of high-quality total DNA from cultured cells or tissue.