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65 výsledky pro Pufry extrakční

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Pufry extrakční

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.

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VWR® Lysis Buffer, Hypotonic

VWR® Lysis Buffer, Hypotonic

Supplier: VWR Chemicals

Detergent-free buffer for isolation of proteins from tissue culture cells. Cell lysis and the subsequent isolation of the crude cytoplasmic fraction completed in less than 30 m.

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Extraction reagents

Supplier: Enzo Life Sciences

Concentrated lysis buffer for preparation of cell and tissue extracts.

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J.T.Baker® Cell Lysis Solution, Biotech Reagent

J.T.Baker® Cell Lysis Solution, Biotech Reagent

Supplier: Avantor

J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.

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Research buffers and reagents

Research buffers and reagents

Supplier: G-Biosciences

These DNase- and RNase-free buffers and reagents are designed for high quality research.

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RIPA cell lysis buffer 2

Supplier: Enzo Life Sciences

RIPA cell lysis buffer 2, Enzo Life Sciences

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Gram Negative lysis buffer

Gram Negative lysis buffer

Supplier: G-Biosciences

Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including E.coli strains.

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E.Z.N.A.® RNALock stabilizer reagent

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® RNALock Stabilizer Reagent is an innovative reagent which rapidly lyses cells and tissues to inactivate RNases at room temperature. Simply submerge the samples, such as cell cultures, animal tissues, or white blood cells into the RNALock Stabilizer Reagent. The intact cellular RNA will be preserved even at elevated temperature. Samples can be stored in this reagent in excess of 12 months at −20 °C.

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Cell lysis buffer, RIPA

Supplier: Enzo Life Sciences

The RIPA liquid cell lysis buffer composes of 50 mM TRIS-hydrogen chloride, pH 7,4, containing 150 mM sodium chloride, 1 mM EDTA, 1 mM EGTA, 1,2% Triton X-100, 0,5% sodium deoxycholate, and 0,1% SDS.

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RBC Lysis Buffer (10X)

RBC Lysis Buffer (10X)

Supplier: Tonbo Biosciences

RBC Lysis Buffer (10X) is a concentrated ammonium chloride-based lysing reagent.

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J.T.Baker® Cell Lysis Solution, Ultrapure Bioreagent

J.T.Baker® Cell Lysis Solution, Ultrapure Bioreagent

Supplier: Avantor

J.T. Baker® Cell Lysis Solution. Optimized multi-component, ready-to-use solution for extraction and recovery of AAV particles from HEK293 or SF9 cells. The product offers quantitative recovery through efficient cell lysis and protection of vector particles against shear stress. The solution is formulated with non-ionic detergents which are octyl phenol ethoxylate free.

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Cell lysis buffer 4, (100 ml), Enzo Life Sciences

Supplier: Enzo Life Sciences

Cell lysis buffer 4, Enzo Life Sciences

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Caspase cell lysis buffer

Supplier: Enzo Life Sciences

Caspase cell lysis buffer, Enzo Life Sciences

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Protein extraction kit, yeast PE LB™

Protein extraction kit, yeast PE LB™

Supplier: G-Biosciences

Yeast PE LB™ kit has been developed for extraction of biologically active, soluble proteins from yeast cells. Yeast PE LB™ is a proprietary improvement on the lyticase (Zymolyase®) based spheroplast preparation and extraction of soluble proteins from yeast cells. Yeast PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins.

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ReadiUse™ Bacterial Cell Lysis Buffer *5X* (10 ml)

ReadiUse™ Bacterial Cell Lysis Buffer *5X* (10 ml)

Supplier: AAT BIOQUEST

Cell lysis refers to the breaking down of cells, and it is often used to analyze specific cellular compositions such as proteins, lipids, nucleic acids, reporter molecules, cell signal molecules and other small biomolecules.

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ReadiUse™ Yeast Cell Lysis Buffer, 5X

ReadiUse™ Yeast Cell Lysis Buffer, 5X

Supplier: AAT BIOQUEST

ReadiUse™ Yeast Cell Lysis Buffer is a biochemical reagent designed for efficient yeast cell lysis and protein solubilisation in laboratory applications.

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Protein extraction kit, Insect PE LB™

Protein extraction kit, Insect PE LB™

Supplier: G-Biosciences

Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.

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Y-PER™ Yeast Protein Extraction Reagent

Y-PER™ Yeast Protein Extraction Reagent

Supplier: Thermo Fisher Scientific

This reagent uses a mild detergent lysis procedure to efficiently release functionally active solubilised proteins.

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Subcellular protein fractionation kit for tissues

Subcellular protein fractionation kit for tissues

Supplier: Thermo Fisher Scientific

This kit is ideal for cell fractionation and enrichment of proteins from five different cellular compartments within two hours.

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ReadiUse™ Viral RNA Lysis Buffer

ReadiUse™ Viral RNA Lysis Buffer

Supplier: AAT BIOQUEST

Current demands for RT-PCR testing of SARS-CoV-2 has led to a worldwide shortage of RNA extraction kits.

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Protein extraction kit, bacterial PE LB™

Protein extraction kit, bacterial PE LB™

Supplier: G-Biosciences

Bacterial PE LB™ kit has been developed for the extraction of biologically active soluble proteins, including recombinant proteins and inclusion bodies, from bacterial cells. Bacterial PE LB™ is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA and RNA) released during cell lysis. The Bacterial PE LB™ lysis system eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.

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Protein extraction kit, tissue PE LB™

Protein extraction kit, tissue PE LB™

Supplier: G-Biosciences

Tissue PE LB™ has been developed for extraction of total biologically active, soluble proteins from animal tissues. Tissue PE LB™ is based on an organic buffer and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Tissue PE LB™. Tissue PE LB™ has been tested for use with a wide variety of fresh and frozen animal tissues.

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Protein extraction kit, mammalian cell PE LB™

Protein extraction kit, mammalian cell PE LB™

Supplier: G-Biosciences

Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.

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Pierce™ DNA Extraction Buffer, Phenol:Chloroform and Tris Buffer

Pierce™ DNA Extraction Buffer, Phenol:Chloroform and Tris Buffer

Supplier: Thermo Fisher Scientific

Phenol-chloroform, is phenol supplied premixed with chloroform:isoamyl alcohol (25:24:1) and accompanying bottle of buffer to equilibrate at either pH 6,7 or pH 8,0.

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Subcellular Protein Fractionation Kit for Cultured Cells

Subcellular Protein Fractionation Kit for Cultured Cells

Supplier: Thermo Fisher Scientific

This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.

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DNA/RNA clean-up buffers

Supplier: OMEGA BIO-TEK

Binding buffer XP2, Omega Bio-Tek

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ReadiUse™ Mammalian Cell Lysis Buffer *5X*

ReadiUse™ Mammalian Cell Lysis Buffer *5X*

Supplier: AAT BIOQUEST

All our ReadiUse™ reagents require minimal hands-on time.

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Y-PER™ plus Yeast Protein Extraction Reagent, Dialysable

Y-PER™ plus Yeast Protein Extraction Reagent, Dialysable

Supplier: Thermo Fisher Scientific

This extraction reagent is formulated to effectively lyse yeast and bacterial cells to extract and solubilise proteins for analysis.

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RIPA lysis and extraction buffer, Pierce™

RIPA lysis and extraction buffer, Pierce™

Supplier: Thermo Fisher Scientific

The RIPA lysis and extraction buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent for cultured mammalian cells.

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Lysis buffer, CTAB

Lysis buffer, CTAB

Supplier: PanReac AppliChem

Cationic detergent cetyltrimethylammonium bromide (CTAB) is used to liberate and complex with total cellular nucleic acids.

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IP lysis buffer, Pierce™

IP lysis buffer, Pierce™

Supplier: Thermo Fisher Scientific

The IP lysis buffer is a mammalian whole cell lysis reagent based on a modified RIPA buffer formulation without SDS.

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