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Hledali jste: Enzymy

Enzymy

Enzymes accelerate, or catalyse, chemical reactions, and they are known to catalyse more than 5,000 biochemical reaction types. Most enzymes are proteins, although a few are catalytic RNA molecules. Choose specific enzymes for cleaving bonds, removing genomic DNA from RNA preparations, for producing fragments of proteins, or for use in ion exchange chromatography. Enzymes are used in the chemical industry and other industrial applications when extremely specific catalysts are required.

Collagenase, MP Biomedicals

Supplier: MP Biomedicals

Collagenase can be solubilised in calcium-free solutions such as Hank's Balanced Salts or Earles Balanced Salt Solution.

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Bovine carbonic anhydrase (from Erythrocytes), MP Biomedicals

Supplier: MP Biomedicals

Soluble in distilled water or dilute buffer.

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Crotalus adamanteus Phosphodiesterase I, MP Biomedicals

Supplier: MP Biomedicals

Lyophilised in vials containing approximately 5 mg dry wt. and at least 100 units/vial (20 to 40 units/mg dry wt.).

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Macerozyme, MP Biomedicals

Supplier: MP Biomedicals

The amount of macerating activity on potato tuber disks as determined by weight loss at 40 °C, pH 5,0.

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Bacillus cereus beta-Lactamase, MP Biomedicals

Supplier: MP Biomedicals

Activity: Each vial contains 500 units β-Lactamase I and 50 units β-Lactamase II Unit Definition: One unit is defined as the amount of enzyme which will hydrolyse 1,0 micromole of benzyl penicillin and 1,0 micromole of cephalosporin C per minute at 25 °C in the presence of EDTA pH 7,0.

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Pyruvate kinase, MP Biomedicals

Supplier: MP Biomedicals

Dissolves readily at 5 mg/ml in 0,06 M Tris/HCI pH 7,4 to give a clear colorless solution.

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Streptococcus (Lancefield group C strain) streptokinase, MP Biomedicals

Supplier: MP Biomedicals

Streptokinase catalyzes hydrolysis of amide linkages. Forms a 1:1 complex with plasminogen which is then converted to streptokinase-plasmin. Both streptokinase-plasminogen and streptokinase-plasmin complexes can activate plasminogen. As a result of its interaction with human or rabbit plasminogen, streptokinase undergoes specific fragmentation.

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Catalase, MP Biomedicals

Supplier: MP Biomedicals

Dissolves readily at 5 mg/ml in 0,05 M sodium/potassium phosphate pH 7,0 giving a clear green solution.

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Pig trypsin (from pancreas), MP Biomedicals

Pig trypsin (from pancreas), MP Biomedicals

Supplier: MP Biomedicals

Trypsin-EDTA solution is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation due to its digestive strength.

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Human catalase (from Erythrocytes), MP Biomedicals

Supplier: MP Biomedicals

Catalase activates the decomposition of hydrogen peroxide, a reactive oxygen species, into water and oxygen.

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Neuraminidase (from Bacteria), MP Biomedicals

Supplier: MP Biomedicals

Neuraminidase of non-pathogenic streptococci, is prepared from the culture filtrate by Ammonium Sulfate precipitation and Ion-exchange chromatography.

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Wheat germ acid phosphatase, MP Biomedicals

Supplier: MP Biomedicals

Acid phosphatase (APase) non-specifically catalyses the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate. It is used to study the production, transport, and recycling of phosphate and the metabolic and energy transduction processes of the cell.

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Leuconostoc mesenteroides Glucose-6-Phosphate dehydrogenase, MP Biomedicals

Supplier: MP Biomedicals

Glucose-6-phosphate dehydrogenase (G6PD or G6PDH) is a cytosolic enzyme in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH).

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Cellulase (from Trichoderma virde), MP Biomedicals

Supplier: MP Biomedicals

Readily soluble in water.

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Arthrobacter luteus zymolyase, MP Biomedicals

Arthrobacter luteus zymolyase, MP Biomedicals

Supplier: MP Biomedicals

Zymolyase 100T is used to lyse living yeast cell walls to produce protoplast or spheroplast of various strains of yeast cells. It may also be used for glucan hydrolysis.

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Tritirachium album proteinase K, MP Biomedicals

Supplier: MP Biomedicals

Suitable for both protein and nucleic acid isolation. Exhibits proteolytic activity on proteins, peptides, glycoproteins, amides and esters. Also active with nitroanilides of amino acids with protected amino groups, excluding arginine. Useful in the isolation of DNA and RNA, in the analysis of membrane structures and protein structure. Proteolytic activity: >30mAnson Units/mg Unit definition: One Anson unit is the amount of enzyme which liberates 1 µmol of Folin-positive amino acids per minute at pH 7,5 and 35 °C, using hemoglobin as substrate.

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Streptomyces hyalurolyticus hyaluronidase, MP Biomedicals

Supplier: MP Biomedicals

The hyaluronidase is purified from Streptomyces hyalurolyticus. This enzyme belongs to the endo-beta-hexosaminidase and endo-eliminase type enzyme that produces oligo-saccharide (unsaturated tetraose and pentose) by hydrolysing the beta-1,4-linkage of hyaluronic acid. It is stable in both high temperature and acid or alkaline range. It has high substrate specificity and hydrolyzes hyaluronic acid only in mucopolysaccharide.

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Flavobacterium meningosepticum Proline specific endopeptidase, MP Biomedicals

Supplier: MP Biomedicals

Proline specific endopeptidase specifically cleaves peptide bonds on the carboxy side of proline residues.

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Bovine deoxyribonuclease i (from Pancreas), MP Biomedicals

Supplier: MP Biomedicals

Dissolve at a concentration of 1 mg/ml. Dilute further to a concentration of 20 to 60 U/ml immediately before the assay.

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Pig Diaphorase (from Heart), MP Biomedicals

Supplier: MP Biomedicals

Pig Diaphorase (from Heart)

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Recombinant Vaccinia virus capping enzyme (from E. coli)

Supplier: Abnova

Recombinant Vaccinia virus capping enzyme (from E. coli)

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Recombinant Vaccinia virus capping enzyme (from E. coli)

Supplier: Abnova

Recombinant Vaccinia virus capping enzyme (from E. coli)

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Recombinant Bovine ENTEROKINASE (from P. pastoris), His-tag

Supplier: Abnova

Recombinant Bovine ENTEROKINASE (from P. pastoris), His-tag

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Human native collagenase type 3 (from Clostridium histolyticum)

Human native collagenase type 3 (from Clostridium histolyticum)

Supplier: Abnova

Collagenases are enzymes that break the peptide bonds in collagen.

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Papain, MP Biomedicals

Supplier: MP Biomedicals

One unit will hydrolyse 1,0 µmole of N-alpha-benzoyl-L-arginine ethyl ester (BAEE) per minute at 25 °C and pH 6,2.

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Aldolase, MP Biomedicals

Supplier: MP Biomedicals

Aldolase is a tetrameric protein. It catalyses a key reaction in glycolysis and energy production:D-Fructose 1,6-bisphosphate aldolase → dihydroxyacetone phosphate + D-glyceraldehyde-3-phosphate. Aldolase is present in all animal tissue and in most microorganisms. There are two classes of aldolases. Class I aldolase is found in animal and higher plant tissue. Class II aldolase is found in primitrive cells such as yeasts and bacteria. Class I aldolase is characterised by not requiring a bivalent metal cofactor and the formation of a ketimine Schiff base intermediate with the substrate dihydroxyacetone phosphate. Class II aldolase requires a metal cofactor and is inhibited by EDTA. Three types of aldolase exist in animal tissue. The major form, type A is found in muscle; type B is found in liver tissue and type C (plus some type A) is found in brain tissue. Aldolase forms five isozymes which may to various degrees be organ specific.

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Alcohol dehydrogenase, MP Biomedicals

Supplier: MP Biomedicals

Dissolves readily at 5 mg/ml in 0,01 M Sodium Phosphate pH 7,5 to give a clear colourless solution.

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Alcaligenes sp. Choline Oxidase, MP Biomedicals

Supplier: MP Biomedicals

Activity: 10 units/mg solid or more (containing approx. 20% of stabilisers). Unit definition: One unit causes the formation of one micromole of hydrogen peroxide per minute at pH 8,0 at 37 °C. Ref.: P.J.G. Mann and J.H. Quastel, Biochem. J., 31: 869 (1937)

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Recombinant mRNA Cap 2'-O-methyltransferase (from E. coli)

Supplier: Abnova

Recombinant mRNA Cap 2'-O-methyltransferase (from E. coli)

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Recombinant Poly(A) Polymerase (from E. coli)

Supplier: Abnova

Recombinant Poly(A) Polymerase (from E. coli)

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