Hledali jste: Enzymy
Enzymes accelerate, or catalyse, chemical reactions, and they are known to catalyse more than 5,000 biochemical reaction types. Most enzymes are proteins, although a few are catalytic RNA molecules. Choose specific enzymes for cleaving bonds, removing genomic DNA from RNA preparations, for producing fragments of proteins, or for use in ion exchange chromatography. Enzymes are used in the chemical industry and other industrial applications when extremely specific catalysts are required.
Cucumis sp. ascorbate oxidase, MP Biomedicals
Supplier: MP Biomedicals
Ascorbate Oxidase is an enzyme belonging to the family of oxidoreductases that catalyses the reaction: Ascorbic acid +¹/₂ O₂ ascorbate oxidase → Dehydroascorbic acid +H₂O.
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Alcaligenes sp. Choline Oxidase, MP Biomedicals
Supplier: MP Biomedicals
Activity: 10 units/mg solid or more (containing approx. 20% of stabilisers). Unit definition: One unit causes the formation of one micromole of hydrogen peroxide per minute at pH 8,0 at 37 °C. Ref.: P.J.G. Mann and J.H. Quastel, Biochem. J., 31: 869 (1937)
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Recombinant mRNA Cap 2'-O-methyltransferase (from E. coli)
Supplier: Abnova
Recombinant mRNA Cap 2'-O-methyltransferase (from E. coli)
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Recombinant Poly(A) Polymerase (from E. coli)
Supplier: Abnova
Recombinant Poly(A) Polymerase (from E. coli)
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Synthetic Yeast Inorganic Pyrophosphatase (from E. coli)
Supplier: Abnova
Synthetic Yeast Inorganic Pyrophosphatase (from E. coli)
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Alcohol dehydrogenase, MP Biomedicals
Supplier: MP Biomedicals
Dissolves readily at 5 mg/ml in 0,01 M Sodium Phosphate pH 7,5 to give a clear colourless solution.
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Pepsin
Supplier: Thermo Fisher Scientific
Contains either Lactose or Maltodextrin excipient
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Clostridium histolyticum Collagenase type IV (from Clostridium histolyticum), MP Biomedicals
Supplier: MP Biomedicals
Activators: Ca²⁺ is required. Inhibitors: Metal chelating agents such as cysteine, EDTA or o-phenanthroline but not DFP. It is also inhibited by a2-macroglobulin - a large plasma glycoprotein. The human skin enzyme is inhibited by human serum but granulocyte collagenase is not. Human serum contains a2-macroglobulin and a1-antitrypsin that may inhibit certain collagenases.
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Sorbitol dehydrogenase, MP Biomedicals
Supplier: MP Biomedicals
Sorbitol dehydrogenase has been used in a study to investigate osmotic stress induced oxidative damage as a possible mechanism of cataract formation in diabetes.
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Aldolase, MP Biomedicals
Supplier: MP Biomedicals
Aldolase is a tetrameric protein. It catalyses a key reaction in glycolysis and energy production:D-Fructose 1,6-bisphosphate aldolase → dihydroxyacetone phosphate + D-glyceraldehyde-3-phosphate. Aldolase is present in all animal tissue and in most microorganisms. There are two classes of aldolases. Class I aldolase is found in animal and higher plant tissue. Class II aldolase is found in primitrive cells such as yeasts and bacteria. Class I aldolase is characterised by not requiring a bivalent metal cofactor and the formation of a ketimine Schiff base intermediate with the substrate dihydroxyacetone phosphate. Class II aldolase requires a metal cofactor and is inhibited by EDTA. Three types of aldolase exist in animal tissue. The major form, type A is found in muscle; type B is found in liver tissue and type C (plus some type A) is found in brain tissue. Aldolase forms five isozymes which may to various degrees be organ specific.
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Thrombin
Supplier: Cytiva
Thrombin is a protease used to digest fusion proteins prepared from pGEX vectors containing the recognition sequence for thrombin (pGEX-1lT, pGEX-2T, pGEX-2TK, pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3).
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alpha-Amylase, MP Biomedicals
Supplier: MP Biomedicals
One bacterial amylase unit is defined as that quantity of enzyme which will dextrinise one milligram of starch per minute at pH 6,6 and 30 °C.
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Cellulase (from Aspergillus niger), MP Biomedicals
Supplier: MP Biomedicals
Cellulase refers to a group of enzymes which, acting together, hydrolyse cellulose. The b-glucosidases, including cellobiose, are primarily active on the smaller molecular weight cellulose hydrolysates. During cellulose breakdown they are active on the dimers and oligomers of cellulose.
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Neuraminidase, MP Biomedicals
Supplier: MP Biomedicals
Neuraminidase (Sialidase: Acylneuraminyl hydrolase; EC 3.2.1.18) From Arthrobacter ureafaciens lyophilised powder with salts. The salts are composed of sodium-potassium phosphate to give a solution of 10 mM phosphate buffer (pH 7) when enzyme is reconstituted with water to make activity of 1 unit per ml. Activity: >60 units/mg protein for NAN-lactose >25 units/mg protein for bovine submaxillary mucin >20 units/mg protein for colominic acid Protein is determined by the method of Lowry et al. with bovine albumin as a standard.6 Unit definition: One unit will liberate 1,0 µmole of N-acetyl-neuraminic acid (NANA) per minute at pH 5,0 at 37 °C, using either one NAN-lactose, bovine submaxillary mucin, or colominic acid as a substrate.
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Human catalase (from Erythrocytes), MP Biomedicals
Supplier: MP Biomedicals
Catalase activates the decomposition of hydrogen peroxide, a reactive oxygen species, into water and oxygen.
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Catalase, MP Biomedicals
Supplier: MP Biomedicals
Dissolves readily at 5 mg/ml in 0,05 M sodium/potassium phosphate pH 7,0 giving a clear green solution.
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Crotalus adamanteus Phosphodiesterase I, MP Biomedicals
Supplier: MP Biomedicals
Lyophilised in vials containing approximately 5 mg dry wt. and at least 100 units/vial (20 to 40 units/mg dry wt.).
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Recombinant Alkaline Phosphatase (from E. coli)
Supplier: Abnova
Recombinant Alkaline Phosphatase (from E. coli)
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Macerozyme, MP Biomedicals
Supplier: MP Biomedicals
The amount of macerating activity on potato tuber disks as determined by weight loss at 40 °C, pH 5,0.
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Bovine carbonic anhydrase (from Erythrocytes), MP Biomedicals
Supplier: MP Biomedicals
Soluble in distilled water or dilute buffer.
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Collagenase, MP Biomedicals
Supplier: MP Biomedicals
Collagenase can be solubilised in calcium-free solutions such as Hank's Balanced Salts or Earles Balanced Salt Solution.
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Collagenase, MP Biomedicals
Supplier: MP Biomedicals
For typical assay, dissolve enzyme at a concentration of 1 mg/ml in 0,05 M TES with 0,36 mM calcium chloride, pH 7,5. Dilutions run are 1/10 and 1/20 in the above buffer.
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Bacillus cereus beta-Lactamase, MP Biomedicals
Supplier: MP Biomedicals
Activity: Each vial contains 500 units β-Lactamase I and 50 units β-Lactamase II Unit Definition: One unit is defined as the amount of enzyme which will hydrolyse 1,0 micromole of benzyl penicillin and 1,0 micromole of cephalosporin C per minute at 25 °C in the presence of EDTA pH 7,0.
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Pyruvate kinase, MP Biomedicals
Supplier: MP Biomedicals
Dissolves readily at 5 mg/ml in 0,06 M Tris/HCI pH 7,4 to give a clear colorless solution.
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Pig trypsin (from pancreas), MP Biomedicals
Supplier: MP Biomedicals
Trypsin-EDTA solution is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation due to its digestive strength.
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Streptococcus (Lancefield group C strain) streptokinase, MP Biomedicals
Supplier: MP Biomedicals
Streptokinase catalyzes hydrolysis of amide linkages. Forms a 1:1 complex with plasminogen which is then converted to streptokinase-plasmin. Both streptokinase-plasminogen and streptokinase-plasmin complexes can activate plasminogen. As a result of its interaction with human or rabbit plasminogen, streptokinase undergoes specific fragmentation.
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Neuraminidase (from Bacteria), MP Biomedicals
Supplier: MP Biomedicals
Neuraminidase of non-pathogenic streptococci, is prepared from the culture filtrate by Ammonium Sulfate precipitation and Ion-exchange chromatography.
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Wheat germ acid phosphatase, MP Biomedicals
Supplier: MP Biomedicals
Acid phosphatase (APase) non-specifically catalyses the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate. It is used to study the production, transport, and recycling of phosphate and the metabolic and energy transduction processes of the cell.