Hledali jste: Enzymy

Supplier: MP Biomedicals

Ascorbate Oxidase is an enzyme belonging to the family of oxidoreductases that catalyses the reaction: Ascorbic acid +¹/₂ O₂ ascorbate oxidase → Dehydroascorbic acid +H₂O.

Supplier: MP Biomedicals

Activity: 10 units/mg solid or more (containing approx. 20% of stabilisers). Unit definition: One unit causes the formation of one micromole of hydrogen peroxide per minute at pH 8,0 at 37 °C. Ref.: P.J.G. Mann and J.H. Quastel, Biochem. J., 31: 869 (1937)

Supplier: Abnova

Recombinant mRNA Cap 2'-O-methyltransferase (from E. coli)

Supplier: Abnova

Recombinant Poly(A) Polymerase (from E. coli)

Supplier: Abnova

Synthetic Yeast Inorganic Pyrophosphatase (from E. coli)

Supplier: MP Biomedicals

Dissolves readily at 5 mg/ml in 0,01 M Sodium Phosphate pH 7,5 to give a clear colourless solution.

Supplier: Thermo Fisher Scientific

Contains either Lactose or Maltodextrin excipient

Supplier: Thermo Fisher Scientific

CAS No.: 9001-73-4

Supplier: MP Biomedicals

Activators: Ca²⁺ is required. Inhibitors: Metal chelating agents such as cysteine, EDTA or o-phenanthroline but not DFP. It is also inhibited by a2-macroglobulin - a large plasma glycoprotein. The human skin enzyme is inhibited by human serum but granulocyte collagenase is not. Human serum contains a2-macroglobulin and a1-antitrypsin that may inhibit certain collagenases.

Supplier: MP Biomedicals

Sorbitol dehydrogenase has been used in a study to investigate osmotic stress induced oxidative damage as a possible mechanism of cataract formation in diabetes.

Supplier: MP Biomedicals

Aldolase is a tetrameric protein. It catalyses a key reaction in glycolysis and energy production:D-Fructose 1,6-bisphosphate aldolase → dihydroxyacetone phosphate + D-glyceraldehyde-3-phosphate. Aldolase is present in all animal tissue and in most microorganisms. There are two classes of aldolases. Class I aldolase is found in animal and higher plant tissue. Class II aldolase is found in primitrive cells such as yeasts and bacteria. Class I aldolase is characterised by not requiring a bivalent metal cofactor and the formation of a ketimine Schiff base intermediate with the substrate dihydroxyacetone phosphate. Class II aldolase requires a metal cofactor and is inhibited by EDTA. Three types of aldolase exist in animal tissue. The major form, type A is found in muscle; type B is found in liver tissue and type C (plus some type A) is found in brain tissue. Aldolase forms five isozymes which may to various degrees be organ specific.

Supplier: Cytiva

Thrombin is a protease used to digest fusion proteins prepared from pGEX vectors containing the recognition sequence for thrombin (pGEX-1lT, pGEX-2T, pGEX-2TK, pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3).

Supplier: MP Biomedicals

One bacterial amylase unit is defined as that quantity of enzyme which will dextrinise one milligram of starch per minute at pH 6,6 and 30 °C.

Supplier: MP Biomedicals

Cellulase refers to a group of enzymes which, acting together, hydrolyse cellulose. The b-glucosidases, including cellobiose, are primarily active on the smaller molecular weight cellulose hydrolysates. During cellulose breakdown they are active on the dimers and oligomers of cellulose.

Supplier: MP Biomedicals

Neuraminidase (Sialidase: Acylneuraminyl hydrolase; EC 3.2.1.18) From Arthrobacter ureafaciens lyophilised powder with salts. The salts are composed of sodium-potassium phosphate to give a solution of 10 mM phosphate buffer (pH 7) when enzyme is reconstituted with water to make activity of 1 unit per ml. Activity: >60 units/mg protein for NAN-lactose >25 units/mg protein for bovine submaxillary mucin >20 units/mg protein for colominic acid Protein is determined by the method of Lowry et al. with bovine albumin as a standard.6 Unit definition: One unit will liberate 1,0 µmole of N-acetyl-neuraminic acid (NANA) per minute at pH 5,0 at 37 °C, using either one NAN-lactose, bovine submaxillary mucin, or colominic acid as a substrate.

Supplier: MP Biomedicals

Catalase activates the decomposition of hydrogen peroxide, a reactive oxygen species, into water and oxygen.

Supplier: MP Biomedicals

Dissolves readily at 5 mg/ml in 0,05 M sodium/potassium phosphate pH 7,0 giving a clear green solution.

Supplier: MP Biomedicals

Lyophilised in vials containing approximately 5 mg dry wt. and at least 100 units/vial (20 to 40 units/mg dry wt.).

Supplier: Abnova

Recombinant Alkaline Phosphatase (from E. coli)

Supplier: Spectrum Chemical

Commonly used in biochemistry.

Supplier: MP Biomedicals

The amount of macerating activity on potato tuber disks as determined by weight loss at 40 °C, pH 5,0.

Supplier: MP Biomedicals

Soluble in distilled water or dilute buffer.

Supplier: MP Biomedicals

Collagenase can be solubilised in calcium-free solutions such as Hank's Balanced Salts or Earles Balanced Salt Solution.

Supplier: MP Biomedicals

For typical assay, dissolve enzyme at a concentration of 1 mg/ml in 0,05 M TES with 0,36 mM calcium chloride, pH 7,5. Dilutions run are 1/10 and 1/20 in the above buffer.

Supplier: MP Biomedicals

Activity: Each vial contains 500 units β-Lactamase I and 50 units β-Lactamase II Unit Definition: One unit is defined as the amount of enzyme which will hydrolyse 1,0 micromole of benzyl penicillin and 1,0 micromole of cephalosporin C per minute at 25 °C in the presence of EDTA pH 7,0.

Supplier: MP Biomedicals

Dissolves readily at 5 mg/ml in 0,06 M Tris/HCI pH 7,4 to give a clear colorless solution.

Supplier: MP Biomedicals

Trypsin-EDTA solution is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation due to its digestive strength.

Supplier: MP Biomedicals

Streptokinase catalyzes hydrolysis of amide linkages. Forms a 1:1 complex with plasminogen which is then converted to streptokinase-plasmin. Both streptokinase-plasminogen and streptokinase-plasmin complexes can activate plasminogen. As a result of its interaction with human or rabbit plasminogen, streptokinase undergoes specific fragmentation.

Supplier: MP Biomedicals

Neuraminidase of non-pathogenic streptococci, is prepared from the culture filtrate by Ammonium Sulfate precipitation and Ion-exchange chromatography.

Supplier: MP Biomedicals

Acid phosphatase (APase) non-specifically catalyses the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate. It is used to study the production, transport, and recycling of phosphate and the metabolic and energy transduction processes of the cell.