Sady a reagencie pro purifikaci nukleových kyselin

Supplier: VWR Chemicals

Rapid method for DNA extraction and purification from all types of microorganisms in a variety of matrices for molecular biology.

Supplier: VWR Chemicals

Dependable isolation of high quality total cellular DNA from a variety of plant species and tissues. peqGOLD homogeniser columns remove cell debris and improve sample handling following lysis.

Supplier: VWR Chemicals

Purification of single- or double-stranded DNA from PCR or other enzymatic reactions.

Supplier: VWR Chemicals

Reagencie VWR® ExoCleanUp FAST PCR je jednokrokové PCR čisticí činidlo pro optimální výsledky sekvenování, které obsahuje vyváženou kombinaci termolabilní exonukleázy I (HL-ExoI) a rekombinantní alkalické fosfatázy z krevet (rSAP).

Supplier: VWR Chemicals

The peqGOLD DNase I Digest Kit is optimised for use with peqGOLD RNA extraction protocols. Normally DNase I digestion is not required for RNA purified with peqGOLD RNA Mini Columns as our silica-based spin column technology efficiently removes the majority of DNA without enzymatic digestion. However, certain sensitive RNA applications may require further DNA removal.

Supplier: VWR Chemicals

Plasmid Miniprep Kit I for the easy, quick and efficient extraction of highly pure plasmid DNA. Isolate up to 30 µg of high quality plasmid DNA from 1 to 5 ml bacterial cultures in 30 minutes or less. Plasmid DNA obtained with peqGOLD Plasmid Miniprep kits can directly be used for a wide range of downstream applications.

Supplier: OMEGA BIO-TEK

E.Z.N.A.® Plasmid Midi Kit offers a novel and reliable method of isolating up to 200 µg of plasmid DNA in less than 60 minutes. This kit combines HiBind® membrane technology with the time-tested consistency of alkaline SDS lysis of bacterial cells to deliver high-quality plasmid DNA. The DNase/RNase-free HiBind® DNA midi columns operate by eliminating time consuming phenol-chloroform extractions and alcohol precipitations. Plasmid purification using the E.Z.N.A.® Plasmid Midi Kit follows a simple bind-wash-elute procedure thereby making it possible for multiple samples to be processed. Although yields vary according to plasmid copy number, E.coli strain, and conditions of growth, 50 ml of an overnight culture in LB medium will typically produce 200 µg of high-copy number plasmid DNA. When working with low-copy number plasmids the starting culture volume can be increased up to 100 ml. High-quality DNA is ready for immediate use in routine molecular biology applications, such as automated fluorescent sequencing, restriction enzyme digestion, and transfection screening.

Supplier: Thermo Fisher Scientific

DNA purification kit is a simple and rapid system for high quality genomic DNA purification from various sources including: whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues.

Supplier: Abcam

The DNA Concentrator Kit (ab156895) is a complete set of essential components which enables the experimenter to efficiently concentrate DNA from various DNA samples with low concentration of DNA including those from microdissection samples and paraffin-embedded tissues.

Supplier: OMEGA BIO-TEK

The E-Z Select® Robotic Reservoir, 600 ml is designed for holding large volume reagent for use on automated platforms such as Hamilton Microlab STAR.

Supplier: OMEGA BIO-TEK

The Mag-Bind® Plant DNA DS 96 Kit is designed for difficult samples (DS) which are hard to lyse or contain high amounts of polysaccharides and polyphenols. The system uses a CTAB-based lysis buffer which does not require organic solvents. The proprietary binding system prevents inhibitors from binding to the magnetic beads. The wash buffer system further removes polysaccharides, phenolic compounds and enzyme inhibitors from plant tissue lysate. An optional rebinding step is included with the kit but most samples do not require the additional treatment.

Supplier: Abcam

Mitochondria/cytosol fractionation kit provides a unique formulation of reagents for the effective isolation of highly enriched mitochondrial fractions from the cytosolic fraction of mammalian cells, including both apoptotic and non-apoptotic cells. - Cited in over 110 publications - Isolates mitochondrial and cytosolic fractions for studying apoptotic pathways and signal transduction - Simple and easy procedure for fractionation - Suitable for detecting translocation of factors between fractions via Western blotting (WB), ELISA, or other assays

Supplier: OMEGA BIO-TEK

Díky přizpůsobení Hi-Bind® technologie 96-jamkovým destičkám nabízí souprava E-Z 96® Tissue DNA Kit vysoce výkonnou metodu purifikace genomové DNA z krve, bukálních stěrů, myšího ocasu, krysího ocasu, tkání a živočišných buněk a tkání ve formátu 96-jamkové destičky. Purifikovaná DNA je vhodná pro většinu následných aplikací, jako jsou PCR, restrikční enzymatické štěpení a hybridizační techniky.

Supplier: OMEGA BIO-TEK

Isolate viral RNA from nasopharyngeal swab specimens (dry or in VTM) using magnetic beads.

Supplier: OMEGA BIO-TEK

E.Z.N.A.® MicroElute Total RNA Kit provides a rapid and easy method for the isolation of up to 50 µg of total RNA from small amounts of cultured eukaryotic cells, tissues such as as laser dissected samples (LDS) or fine needle aspirates(FNA). Normally, up to 5×10⁵ eukaryotic cells or 5 mg tissue can be used in a single experiment depending on the type of tissue used. This kit allows processing of single or multiple of samples in less than 30 minutes. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifuation, and precipitation with isopropanol or LiCl. Purified RNA can be eluted with 10 to 15 µl nuclease-free water. Purified RNA is ready for most downstream applications such as RT-PCR, Northern blotting, Poly A+ purification, nuclease protection and invitro translation.

Supplier: OMEGA BIO-TEK

Souprava E.Z.N.A.® tissue DNA kit nabízí jednoduchou, rychlou a cenově výhodnou metodu pro izolaci DNA ze široké škály typů vzorků, včetně čerstvých nebo zmražených živočišných buněk a tkání. Po lyzi buněk může být purifikace DNA hotová do 30 minut. Najednou může být pomocí jednoduchého E.Z.N.A.® tissue DNA protokolu snadno zpracováno až 30 mg tkáně. Souprava využívá centrifugační kolonky a umožňuje zpracovávat jeden vzorek či více vzorků současně. Nejsou nutné extrakce fenolem/chloroformem ani časově náročné kroky jako precipitace izopropanolem nebo ethanolem. DNA purifikovaná za využití soupravy E.Z.N.A.® tissue DNA kit je vhodná pro většinu následných aplikací, jako jsou PCR, southern blot a restrikční enzymatické štěpení.