79 Results for: "hydrophobic interaction"

Supplier: GE HEALTHCARE

HiTrap™ Butyl-S-FF are prepacked Butyl-S Sepharose™ 6 Fast Flow columns for hydrophobic interaction chromatography (HIC).

Supplier: GE HEALTHCARE

HiScreen Octyl FF columns are prepacked with Octyl Sepharose 4 Fast Flow hydrophobic interaction chromatography (HIC) resins. The columns are an excellent choice for method optimization and parameter screening.

Supplier: GE HEALTHCARE

HiScreen Butyl S-FF columns are prepacked with Butyl Sepharose 6 Fast Flow hydrophobic interaction chromatography (HIC) resins. The columns are an excellent choice for method optimization and parameter screening.

Supplier: GE HEALTHCARE

Octyl Sepharose™ 4 Fast Flow resin is based on cross-linked 4% agarose. The aliphatic ligand is immobilised to the base matrix with an ether linkage. The ligand contains no charged groups, making true hydrophobic interaction chromatography possible, without interfering ionic effects.

Supplier: TOSOH BIOSCIENCE

These are polymethacrylate-based columns with a choice of three ligands (ether, phenyl or butyl) with varied hydrophobicities from low to high. Suitable for LC-MS applications.

Supplier: BIO RAD

Bio-Beads SM-2 adsorbents, non polar polysterene, 300 - 1,180 µm bead size, for organics < 2000 MW, 100 g

Supplier: GE HEALTHCARE

SOURCE 15PHE 4.6/100 PE is a Tricorn high performance column pre-packed with SOURCE 15PHE.

Supplier: GE HEALTHCARE

Butyl-S Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified with butyl groups via a thio-ether linkage. This resin is designed for the binding and elution of relatively strong hydrophobic molecules at comparatively low salt concentrations.

Supplier: HAMILTON BONADUZ

PRP-X500 is a superficially porous polymeric anion exchange column designed for the separation, purification and isolation of proteins, peptides and DNA/RNA. The methacrylate polymeric coating of the PRP-X500 provides a more hydrophilic surface, preventing hydrophobic interaction sample losses.

Supplier: Thermo Fisher Scientific

These columns are used for the high resolution separation of the proteins and peptides.

Supplier: MP BIOMEDICALS

Guanidine Hydrochloride is a protein denaturant and thus having an important role in molecular weight determinations.
Guanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine HCl is used in the isolation of RNA to dissociate the nucleoprotein into its nucleic acid and protein moieties. It is an inhibitor of RNase. Highly concentrated (6 - 8 M) Guanidine HCl solutions are used to denature native globular proteins. It apparently disrupts hydrogen bonds which hold the protein in its unique structure. However, there also is evidence suggesting that guanidine hydrocholoride may disrupt hydrophobic interactions by promoting the solubility of hydrophobic residues in aqueous solutions.

Supplier: GE HEALTHCARE

Capto™ Phenyl (high sub) is used in the capture and intermediate stages of protein purification.

Supplier: GE HEALTHCARE

Butyl Sepharose™ HP media is particularly well suited for intermediate purification and polishing steps providing high resolution due to the small particle size.

Supplier: GE HEALTHCARE

Capto™ Butyl is used in the capture and intermediate stages of protein purification. The combination of high capacity, high flow rate and low back pressure reduces process cycle times.

Supplier: GE HEALTHCARE

Butyl Sepharose 4 Fast Flow is part of the Sepharose Fast Flow HIC platform, which has been an industrial standard for HIC chromatography during recent decades.

Supplier: CYTIVA PALL

Naturally hydrophobic PVDF, ideal for a wide variety of protein-analysis applications. The family of FluroTrans® media are white, microporous solid phase supports that bind proteins tenaciously via hydrophobic interactions. FluoroTrans® membrane is optimised for N-terminal protein sequencing. FluoroTrans® W membranes are optimised for Western transfer applications. They allow for sensitive protein detection with low background and very low protein burn-through. Immobilised proteins can be visually detected with Coomassie® Blue, Amido black, Ponceau S, and colloidal gold.

Supplier: GE HEALTHCARE

Capto™ SP ImpRes is a strong cation exchange bioprocessing medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective intermediate and polishing protein purification.

Supplier: CORNING SCIENCE PRODUCTS

PS. Designed to reduce well-to-well crosstalk during fluorescent and luminescent assays.

Supplier: GE HEALTHCARE

Phenyl Sepharose 6 Fast Flow is a well-established, standard aromatic HIC medium for capture and intermediate purification requiring low to medium hydrophobicity.

Supplier: GE HEALTHCARE

HiTrap Capto MMC is prepacked with Capto MMC, a multimodal BioProcess ion exchange resin. The multimodal functionality gives a different selectivity compared with traditional ion exchangers and it binds proteins at high or low ionic strength.

Supplier: YMC

The YMC30 is a very lipophilic phase with exceptional selectivity towards isomeric structures. The C30 ligands provide sufficient phase thickness to enhance interaction with long chain molecules. Particularly suited for the separation of carotenoids.

Supplier: PHENOMENEX

C8 column provides less hydrophobic retention than C18, but the density of the ligand bonding creates more steric based selectivity. The C8 columns are generally better hydrogen bond acceptors and better for acidic compounds.

Supplier: MACHEREY-NAGEL

NUCLEOSIL® C8 and C8 ec are octyl modified silica phases for HPLC.

Supplier: Thermo Fisher Scientific

PS, treated to produce high-capacity antibody-binding characteristics for immunoassay applications.

Supplier: Avantor

Avantor® ACE® C18-Amide is a uniquely designed stationary phase offering improved retention of polar compounds, enhanced phenolic resolution and alternative selectivity to C18 bonded columns. These stainless steel columns are available in a wide range of particle sizes and dimensions from capillary to preparative.

Supplier: Avantor

Avantor® ACE® C18-Amide is a uniquely designed stationary phase offering improved retention of polar compounds, enhanced phenolic resolution and alternative selectivity to C18 bonded columns. These stainless steel columns are available in a wide range of particle sizes and dimensions from capillary to preparative.

Supplier: Avantor

Avantor® ACE® Excel® C18-Amide is a uniquely designed stationary phase offering improved retention of polar compounds, enhanced phenolic resolution and alternative selectivity to C18 bonded columns. These stainless steel columns are available in a wide range of particle sizes and dimensions from capillary to preparative.

Supplier: MACHEREY-NAGEL

NUCLEOSIL® CN and CN-RP are cyano (nitrile) modified silica phases for HPLC.

Supplier: MACHEREY-NAGEL

NUCLEOSIL® C18 HD is an octadecyl modified silica phase with a high density coverage, using for HPLC.

Supplier: HAMILTON BONADUZ

PRP-X600 is a superficially porous weak-base anion exchange support that separates DNA oligomers according to negative charge.