"animal sera"

Supplier: VWR Seradigm

Australia Origin FBS is widely considered the gold standard for safety and regulatory compliance by cell culturists around the world. Avantor Seradigm Australia Origin FBS combines outstanding performance and quality with ease of regulatory access globally, suitable for a wide variety of applications.

Supplier: Stemcell Technologies

Animal component-free, serum-free, glutamine-free 40X supplement for cloning during CHO cell line development.

Supplier: CYTIVA HYCLONE

HyClone™ donor horse serum, designed to supplement cell growth, is collected from selected donor herds that receive regular veterinary inspection and care.

Supplier: PROMEGA

FuGENE® HD transfection reagent has a non-liposomal formulation designed to transfect DNA into a wide variety of cell lines with high efficiency and low toxicity. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex. Additionally, the FuGENE® HD Transfection Reagent has been shown to support transfection in chemically defined media and does not contain any animal-derived components.

Supplier: EBIOSCIENCE

5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser BAFF is a membrane bound cytokine expressed by B cell lineage cells. The soluble form of the cytokine binds to the receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. BAFF is a potent B cell activator, and important for the proliferation and differentiation of B cells. BAFF is also expressed by T lymphocytes and has been shown to promote T cell activation and survival. Isoforms representing alternatively spliced, and glycosylated variants have been identified.

Supplier: VWR Collection

Designed for the storage of biological material, human or animal cells, at temperatures as low as –196 °C (should only be used in the gas phase of liquid nitrogen).

Supplier: Stemcell Technologies

Animal component-free, defined cryopreservation medium with 10% DMSO.

Supplier: PROMEGA

ViaFect™ transfection reagent is designed to transfect DNA into a wide variety of cell lines with high efficiency and low toxicity. ViaFect™ Transfection Reagent has been shown to transfect cell lines that are traditionally thought of as difficult to transfect (such as hematopoietic, primary and iPSC stem cell lines) with relatively high efficiency. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex.

Supplier: POLYPLUS

The FectoPRO® Transfection Kit is specifically designed for enhanced Transient Gene Expression (TGE) in suspension CHO and HEK-293 cells in various serum-free media, using low DNA amount (<1 μg/ml of cell culture).

Supplier: VWR Chemicals

VWR® dehydrated culture media that has been granulated offers both convenience and safety benefits. The correct dissolution of culture media is critical to achieving the best performance. Granulated culture media saves time in the preparation of ready to use culture media. In addition, granulation reduces dust generation, so handling the product is more pleasant and safer for the user, making it a much cleaner and safer working environment.

Supplier: SIGMAALDRICH

Cell-free protein expression (CFPE) is the production of recombinant proteins in solution using biomolecular translation machinery extracted from cells of different species, such as E. Coli, wheat germ, Leishmania tarentolae, rabbit reticulocyte, insect or human cells. CFPE system from wheat germ extract is suitable for the expression of both prokaryotic and eukaryotic proteins.It usually does not require codon optimization even for the expression of transcripts with a high A/T or G/C content.