T4 RNA ligase (from Escherichia coli)
Supplier: PROMEGA
T4 RNA ligase catalyses the ATP-dependent ligation of single-stranded RNA or DNA onto the 5'-phosphoryl termini of single-stranded RNA or DNA. The enzyme, purified from recombinant E. coli CA4 (RNase I-deficient), has an apparent molecular weight of 43,5 kDa. It also catalyses the addition of [5'-³²P] nucleoside 3',5'-bis (phosphate) onto single-stranded RNA.
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IdeS Protease
Supplier: PROMEGA
IdeS Protease is an immunoglobulin-degrading enzyme from Streptococcus pyogenes (IdeS). IdeZ Protease is an immunoglobulin-degrading enzyme from Streptococcus equi subspecies zooepidemicus.
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Buffer, TE solution (TRIS-EDTA buffer)
Supplier: PROMEGA
Cloning Buffer, TE buffer solution (TRIS-EDTA buffer)
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CellTiter 96® AQueous MTS Reagent Powder
Supplier: PROMEGA
CellTiter 96® AQueous MTS Reagent Powder is a novel tetrazolium compound used in colorimetric assays to determine the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays.
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Transfection reagent, ViaFect™
Supplier: PROMEGA
ViaFect™ transfection reagent is designed to transfect DNA into a wide variety of cell lines with high efficiency and low toxicity. ViaFect™ Transfection Reagent has been shown to transfect cell lines that are traditionally thought of as difficult to transfect (such as hematopoietic, primary and iPSC stem cell lines) with relatively high efficiency. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex.
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水 nuclease-free
Supplier: PROMEGA
Nuclease-Free Water is an essential component of molecular biology experiments.
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dNTP mix
Supplier: PROMEGA
dNTP Mix is a solution containing sodium salts of dATP, dCTP, dGTP and dTTP, each at 10 mM in water at pH 7,5. The total concentration of nucleotides is 40 mM. One microliter of the dNTP Mix in a 50 μl reaction will give a final dNTP concentration of 200 μM for each dNTP.
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GoTaq® DNA polymerase
Supplier: PROMEGA
GoTaq® DNA polymerase is a proprietary formulation of Taq DNA polymerase that gives robust amplification equal to and, in some cases superior to that of standard Taq polymerase.
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Luciferase assay systems
Supplier: PROMEGA
The Luciferase Assay System is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. Linear results are seen over at least eight orders of magnitude of enzyme concentration, and less than 10 to 20 moles of luciferase can be measured under optimal conditions. Generally, 100-fold greater sensitivity can be achieved over the chloramphenicol acetyltransferase (CAT) assay.
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Trypsin gold, MS grade
Supplier: PROMEGA
Trypsin Gold, mass spectrometry grade, has been manufactured to provide maximum specificity. Lysine residues in the porcine trypsin have been modified by reductive methylation, yielding a highly active and stable molecule that is extremely resistant to autolytic digestion. The specificity of the purified trypsin is further improved by TPCK treatment, which inactivates chymotrypsin. The treated trypsin is then purified by affinity chromatography and lyophilised.
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Oxidative stress assays, GSH/GSSG-Glo™ assay
Supplier: PROMEGA
The GSH/GSSG-Glo™ Assay is a luminescence-based system for the detection and quantification of total glutathione (GSH +GSSG), GSSG and GSH/GSSG ratios in cultured cells. A change in GSH levels is important in the assessment of toxicological responses and is an indicator of oxidative stress, potentially leading to apoptosis or cell death. The assay provides a simple, rapid multiwell-plate format where stable luminescent signals are correlated with either the total GSH or the GSSG concentration of a sample directly in culture wells.
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Wheat germ extract
Supplier: PROMEGA
Wheat germ extract contains the cellular components necessary for protein synthesis (tRNA, ribosomes, initiation, elongation and termination factors).
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Magnetic separation stand, MagneSphere® technology
Supplier: PROMEGA
The MagneSphere® Technology magnetic separation stands can be used in conjunction with any of the PolyATtract® systems and are ideal for applications requiring multiple paramagnetic isolations of biomolecules.
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Thermolysin
Supplier: PROMEGA
Thermolysin is a thermostable metalloproteinase. The high digestion temperature stability of this enzyme may be useful as an alternative to denaturants to improve digestion of proteolytically resistant proteins.
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Luciferase assay system, Nano-Glo®
Supplier: PROMEGA
The Nano-Glo® Luciferase Assay System provides a simple, single-addition reagent that generates a glow-type signal in the presence of NanoLuc® luciferase with a half-life of approximately 120 minutes in commonly used tissue culture media.
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ATP assay system, ENLITEN®
Supplier: PROMEGA
The ENLITEN® ATP Assay System Bioluminescence Detection Kit is designed for the rapid and quantitative measurement of adenosine 5´-triphosphate (ATP) levels for the indirect detection of biocontamination.
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兔抗Anti-Mouse IgG (H+L), HRP Conjugate 1 * 300 µl
Supplier: PROMEGA
兔抗Anti-Mouse IgG (H+L), HRP Conjugate 1 * 300 µl
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pFN31A Nluc CMV-Hygro Flexi®载体 1 * 1 件
Supplier: PROMEGA
pFN31A Nluc CMV-Hygro Flexi®载体 1 * 1 件