Supplier: PROMEGA

The CellTiter-Glo® Luminescent Cell Viability Assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, an indicator of metabolically active cells. It is designed for use with multiwell formats, making it ideal for automated high-throughput screening (HTS), cell proliferation and cytotoxicity assays.

Supplier: PROMEGA

Trypsin is a serine protease that specifically cleaves at the carboxylic side of lysine and arginine residues. The stringent specificity of trypsin is essential for protein identification.

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Ideal for use in Western Blot, ELISA and Dot Blot.

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RNasin® Ribonuclease inhibitors are widely used protection against RNases worldwide.

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TRIS HCl (tris(hydroxymethyl)aminomethane hydrochloride)

Supplier: PROMEGA

The 100 bp DNA ladder has eleven fragments that range in size from 100 to 1000 bp in 100 bp increments with an additional band at 1500 bp. The 500 bp fragment is present at increased intensity for easy identification. The ladder is dephosphorylated and ready for 5' end-labelling with radioisotopes using T4 Polynucleotide Kinase for visualisation by autoradiography.

Supplier: PROMEGA

Mass spectrometry-compatible yeast and human protein extracts are designed specifically for mass spectrometry applications. The extracts are predigested and cleaned up by solid-phase extraction for immediate use in liquid chromatography/mass spec analysis

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Trypsin is a serine protease that specifically cleaves at the carboxylic side of lysine and arginine residues. The stringent specificity of trypsin is essential for protein identification.

Supplier: PROMEGA

The ONE-Glo™ Luciferase Assay System provides a highly sensitive, robust, homogeneous assay for detection of firefly luciferase reporter gene expression in mammalian cells in 96- and 384-well plates.

Supplier: PROMEGA

GSH-Glo™ Assay is a luminescent-based assay for the detection and quantification of glutathione (GSH) in cells or in various biological samples. The assay is based on the conversion of a luciferin derivative into luciferin in the presence of GSH.

Supplier: PROMEGA

The CellTiter-Glo® 3D Cell Viability Assay is designed for determining cell viability in 3D microtissue spheroids. The assay reagent penetrates large spheroids and has increased lytic capacity allowing more accurate determination of viability compared to other assay methods.

Supplier: PROMEGA

FuGENE® HD transfection reagent has a non-liposomal formulation designed to transfect DNA into a wide variety of cell lines with high efficiency and low toxicity. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex. Additionally, the FuGENE® HD Transfection Reagent has been shown to support transfection in chemically defined media and does not contain any animal-derived components.

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The CytoTox 96® Non-Radioactive Cytotoxicity Assay quantitatively measures lactate dehydrogenase (LDH), a stable cytosolic enzyme that is released upon cell lysis, in much the same way as [⁵¹Cr] is released in radioactive assays.

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The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay is a homogeneous, colorimetric method for determining the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays.

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PCR Nucleotide Mix is a premixed solution containing the sodium salts of dATP, dCTP, dGTP and dTTP, each at a concentration of 10mM or 25mM in water.

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High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells is commonly performed by batch processing of 96- and 384-well plates. Steady-Glo® Luciferase Assay System is designed for this purpose of providing long-lived luminescence when added to cultured cells. The homogeneous assay provides signal half-lives of over 5 hours in commonly used cell culture media, and does not require sample preprocessing. Throughput rates of several thousand samples per hour may be achieved with high reproducibility under standard laboratory conditions.

Supplier: PROMEGA

T4 RNA ligase catalyses the ATP-dependent ligation of single-stranded RNA or DNA onto the 5'-phosphoryl termini of single-stranded RNA or DNA. The enzyme, purified from recombinant E. coli CA4 (RNase I-deficient), has an apparent molecular weight of 43,5 kDa. It also catalyses the addition of [5'-³²P] nucleoside 3',5'-bis (phosphate) onto single-stranded RNA.

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IdeS Protease is an immunoglobulin-degrading enzyme from Streptococcus pyogenes (IdeS). IdeZ Protease is an immunoglobulin-degrading enzyme from Streptococcus equi subspecies zooepidemicus.

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Cloning Buffer, TE buffer solution (TRIS-EDTA buffer)

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CellTiter 96® AQueous MTS Reagent Powder is a novel tetrazolium compound used in colorimetric assays to determine the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays.

Supplier: PROMEGA

The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantisation of a stable luminescent signal from two reporter genes in a single sample. The system is optimised for batch processing both 96- and 384-well plates and is compatible with a wide variety of mammalian cell culture media.

Supplier: PROMEGA

Mouse genomic DNA is isolated from whole blood from disease-free mice.

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Human Genomic DNA is isolated from multiple anonymous donors.

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The CellTiter 96® AQueous One Solution Cell Proliferation Assay is a colorimetric method for determining the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays.

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The Griess Reagent System measures nitrite (NO²⁻), which is one of two primary stable and non volatile breakdown products of nitric oxide (NO).

Supplier: PROMEGA

The Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing two reporter assays. In the DLR™ Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis or sea pansy) luciferases are measured sequentially from a single sample.

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This EDTA solution is a chelator of divalent cations and is suitable for biochemistry and molecular biology applications

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Western Blue® stabilised substrate for alkaline phosphatase is a stable, ready to use substrate for Western blots and immunoscreening.

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ViaFect™ transfection reagent is designed to transfect DNA into a wide variety of cell lines with high efficiency and low toxicity. ViaFect™ Transfection Reagent has been shown to transfect cell lines that are traditionally thought of as difficult to transfect (such as hematopoietic, primary and iPSC stem cell lines) with relatively high efficiency. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex.

Supplier: PROMEGA

Nuclease-Free Water is an essential component of molecular biology experiments.