"Ligases"
T4 DNA ligase
Supplier: GE HEALTHCARE
T4 DNA ligase catalyses the formation of a phosphodiester bond between the 5'-phosphoryl group and the 3'-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction.
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T4 RNA ligase (from Escherichia coli)
Supplier: PROMEGA
T4 RNA ligase catalyses the ATP-dependent ligation of single-stranded RNA or DNA onto the 5'-phosphoryl termini of single-stranded RNA or DNA. The enzyme, purified from recombinant E. coli CA4 (RNase I-deficient), has an apparent molecular weight of 43,5 kDa. It also catalyses the addition of [5'-³²P] nucleoside 3',5'-bis (phosphate) onto single-stranded RNA.
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琼脂糖 IEF
Supplier: GE HEALTHCARE
Agarose with low electroendosmosis (EEO) is recommended for most DNA/RNA applications.
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Restriction enzymes, XbaI, FastDigest™, Fermentas
Supplier: Thermo Fisher Scientific
FastDigest XbaI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest green reaction buffers.
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T4 DNA 连接酶 (5 U/µL) 1 * 1 KIT
Supplier: Thermo Fisher Scientific
T4 DNA 连接酶 (5 U/µL) 1 * 1 KIT
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T4 DNA 连接酶 (5 U/μL) 1 * 1 KIT
Supplier: Thermo Fisher Scientific
T4 DNA 连接酶 (5 U/μL) 1 * 1 KIT