34 个结果符合:“Assay Kits and Reagents”
Cell Proliferation Labelling Reagent
Supplier: GE HEALTHCARE
The Cell Proliferation Kit is an ICC system for monitoring cell proliferation using monoclonal anti-5-bromo-2'-deoxyuridine (BrdU) to detect BrdU incorporated into cellular DNA.
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Coomassie (Bradford) protein assay kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Coomassie (Bradford) Protein Assay Kit is a ready-to-use, stable formulation of the traditional Bradford assay reagent to measure (A595 nm) total protein concentration compared to a protein standard.
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Pierce™ BCA™ Protein assay kits and reagents
Supplier: Thermo Fisher Scientific
Pierce BCA™ (bicinchoninic acid) reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA™ Protein Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA™ Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.
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Pierce™ Micro BCA™ Protein Assay Kit and Reagents
Supplier: Thermo Fisher Scientific
Micro BCA™ protein assay kit is a specialised version of Pierce™ BCA protein assay for determining the protein concentration of dilute samples.
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Pierce™去污剂兼容型 Bradford 检测试剂盒 1 * 1 KIT
Supplier: Thermo Fisher Scientific
Pierce™去污剂兼容型 Bradford 检测试剂盒 1 * 1 KIT
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试剂 EUTDA CTOTOXICITY ASSAY KICONTAINING 1 * 1 KIT
Supplier: PerkinElmer
试剂 EUTDA CTOTOXICITY ASSAY KICONTAINING 1 * 1 KIT
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Pierce™ Coomassie Plus (Bradford) Protein Assay Reagent
Supplier: Thermo Fisher Scientific
The bradford protein assay is the preferred colorimetric assay for quantifying total protein concentration. The Pierce™ coomassie plus protein assay is a ready-to-use, reducing agent-compatible, improved bradford assay reagent to quickly measure (A595 nm) total protein concentration compared to a protein standard.
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ATP assay system, ENLITEN®
Supplier: PROMEGA
The ENLITEN® ATP Assay System Bioluminescence Detection Kit is designed for the rapid and quantitative measurement of adenosine 5´-triphosphate (ATP) levels for the indirect detection of biocontamination.
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Active GTPase pull-down and detection kits
Supplier: Thermo Fisher Scientific
The Active GTPase Pull-Down and Detection Kits allows the monitoring of changes in GTPase activity levels by enriching and detecting the active (GTP-bound) state of a particular GTPase.
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Nucleic acid labeling kits, Label IT®
Supplier: MIRUS
Label IT® nucleic acid labeling kits are suitable for direct non-enzymatic labeling of DNA and RNA. Permanent, non-destructive modification of nucleic acid residues is ideal for many diverse applications.
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Oxidative stress detection kit, ROS-Glo™ H₂O₂ assay
Supplier: PROMEGA
The ROS-Glo™ H₂O₂ Assay is a homogeneous, fast and sensitive bioluminescent assay that measures the level of hydrogen peroxide (H₂O₂), a reactive oxygen species (ROS), directly in cell culture or in defined enzyme reactions. A derivatised luciferin substrate is incubated with sample and reacts directly with H₂O₂ to generate a luciferin precursor. The ROS-Glo™ detection solution converts the precursor to luciferin and provides Ultra-Glo™ Recombinant Luciferase to produce light signal that is proportional to the level of H₂O₂ present in the sample.
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Caspase-3 assay kits, NucView™ 488
Supplier: BIOTIUM
NucView® 488 caspase-3 substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3 activity within live cells in real time.
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Human IgG ELISA Kit (Colorimetric)
Supplier: NOVUS BIOLOGICALS
Human IgG ELISA kit (colorimetric)
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Molecular detection assay, Salmonella
Supplier: 3M FOOD SAFETY
3M™ Molecular Detection Assay 2 - Salmonella is used with the 3M™ Molecular Detection System for the rapid and specific detection of Salmonella species in enriched food and environmental samples.
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Amersham™ ECL™ Western Blotting Detection Reagent, Cytivia
Supplier: GE HEALTHCARE
Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.
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Oxidative stress assays, GSH/GSSG-Glo™ assay
Supplier: PROMEGA
The GSH/GSSG-Glo™ Assay is a luminescence-based system for the detection and quantification of total glutathione (GSH +GSSG), GSSG and GSH/GSSG ratios in cultured cells. A change in GSH levels is important in the assessment of toxicological responses and is an indicator of oxidative stress, potentially leading to apoptosis or cell death. The assay provides a simple, rapid multiwell-plate format where stable luminescent signals are correlated with either the total GSH or the GSSG concentration of a sample directly in culture wells.
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qScript™ One-Step qRT-PCR kits
Supplier: QIAGEN BEVERLY
Synthesis of cDNA and PCR amplification are carried out in the same tube without opening between procedures. These kits are optimised to deliver maximum RT-PCR efficiency and specificity, enabling unbiased co-amplification of low copy transcripts in the presence of higher copy reference genes. As a result the qScript™ One-Step qRT-PCR Kits offer improved sensitivity over other commercially available qPCR reagents. The qScript™ One-Step Master Mix maximises the activities of both reverse transcriptase and PCR enzymes, while minimising primer-dimer formation. High specificity is assured with AccuStart™ Taq DNA Polymerase, which is bound by monoclonal antibodies for superior hot-start. Upon heating to 95 °C, the antibodies denature irreversibly releasing fully active and unmodified Taq DNA polymerase. qScript™ One-Step qRT-PCR Kits are available for all Real-Time PCR instrument platforms, including those requiring normalisation with ROX reference dye.
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One-Step Fast qRT-PCR kits, qScript™
Supplier: Quantabio
These kits offer a convenient and highly sensitive solution for Real-Time reverse transcriptase PCR (qRT-PCR) of RNA templates using hybridisation probe detection chemistries, such as TaqMan® 5'-hydrolysis probes.
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TMB substrate kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ TMB Substrate Kit contains soluble TMB (3,3´,5,5´-tetramethylbenzidine) that is ideal for sensitive ELISA-based applications. TMB is a chromogenic substrate for horseradish peroxidase. Once oxidised by the enzyme, this substrate yields a blue product that absorbs at 370 nm and 652 nm. Adding sulfuric or phosphoric acid to stop the reaction changes the product to yellow that absorbs at 450 nm.
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PerfeCTa® qPCR FastMix® II
Supplier: QIAGEN BEVERLY
PerfeCTa® qPCR FastMix® II is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa® qPCR FastMix® II is provided as a 2X concentrated ready to use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimise pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.
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qPCR SuperMixes, PerfeCTa®
Supplier: QIAGEN BEVERLY
PerfeCTa® qPCR SuperMixes are 2X concentrated, ready to use reaction cocktails containing all components except primers, probe and template for qPCR.
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PCR beads, illustra™ Ready-To-Go™ RAPD analysis beads
Supplier: GE HEALTHCARE
RAPD reactions are pre-optimized for use with a wide variety of organisms.
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VWR®, Phenol-free total RNA purification kit
Supplier: VWR Chemicals
The phenol-free total RNA purification kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants and viruses.
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PerfeCTa® qPCR ToughMix®
Supplier: QIAGEN BEVERLY
PerfeCTa® qPCR ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues or animal tissues. It is a versatile and robust Real-Time qPCR reagent that provides maximum sensitivity and PCR efficiency with a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa® qPCR ToughMix® contains all required reaction components, except primers, probe(s) and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear plates and helps to minimise pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.
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illustra™ MicroSpin™ S-400 HR columns
Supplier: GE HEALTHCARE
illustra™ MicroSpin™ S-400 HR columns designed for rapid purification of PCR products (>200 bp) from unincorporated primers (<32-mers) and nucleotides using spin-column chromatography.
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illustra™ MicroSpin™ S-300 HR columns
Supplier: GE HEALTHCARE
illustra™ MicroSpin™ S-300 HR columns designed for rapid purification of PCR products (>100 bp) from unincorporated primers (<20-mers) and nucleotides using spin-column chromatography.
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illustra™ MicroSpin™ G-25 columns
Supplier: GE HEALTHCARE
For rapid buffer exchange/desalting of PCR products and other DNAs in a volume of 10 to 100 μl using spin column chromatography.
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RNA isolation, illustra™ RNAspin 96 Kit
Supplier: GE HEALTHCARE
illustra™ RNAspin 96 kit is a flexible, high-throughput solution for total RNA isolation.
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RNAspin Mini Kits
Supplier: GE HEALTHCARE
RNAspin Mini RNA Isolation Kit is a complete RNA purification kit designed for rapid extraction of high-quality total RNA from a wide range of sample types.
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illustra™ ProbeQuant™ G-50 micro columns
Supplier: GE HEALTHCARE
ProbeQuant™ G-50 micro columns use spin column chromatography to provide a fast and reliable method for purifying labelled probes from unincorporated labelled nucleotides. In addition, a portion of the eluted DNA can be counted in a scintillation counter and compared to an unpurified sample to give an approximation of percent incorporation. The columns are designed for use in a microcentrifuge, come prepacked with Sephadex™ G-50 DNA grade and are pre-equilibrated in STE, which means that they are ready to use. They can accommodate samples ranging in volume from 25 μl to 50 μl.