Sample concentrators, Vivaspin®

Vivaspin® sample concentrators are designed for fast, nondenaturing concentration of biological samples by membrane ultrafiltration.

• One-step sample concentration in a single tube for minimal sample handling and reduced sample loss
• Patented dead-stop technology, which ensures that samples cannot be concentrated to dryness and enables direct concentrate recovery
• Vertical PES membrane minimises membrane blockage and tolerates high flow rates
• Compatible pH range is from pH 1 to 9

Up to 30-fold concentration of the sample can be achieved with recovery of the target molecule typically exceeding 95%. The entire process is performed in a single tube with an upper compartment containing sample and lower compartment separated by a semipermeable membrane with a molecular weight cutoff (MWCO) selected by the user. Centrifugation is applied to force solvent through the membrane, leaving a more concentrated sample in the upper chamber.

Vivaspin® sample concentrators cater for sample volumes from 100 μl to 20 ml, with a range of molecular weight cutoff values from M_r 3000 to 100000. For maximum recovery, select a MWCO at least 50% smaller than the molecular size of the species of interest.