Affinity chromatography columns, HiTrap™ rProtein A FF
Product Details & Documents
HiTrap rProtein A FF columns are prepacked with rProtein A Sepharose Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources, including cell supernatants and ascites.
- Quick, convenient preparative purification of monoclonal antibodies
- Well-established protein A-based resin used in many approved MAb processes
- Simple operations with a syringe, pump, or high-performance chromatography system, such as ÄKTA design
Recombinant protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
The recombinant protein A ligand (produced in E. coli) is coupled to Sepharose Fast Flow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.
规格
- 流速:20 ml/min
- 颗粒大小:90 µm
- 容量:1×5 ml
- 说明:HiTrap™ rProtein A FF
- 顺序:04
- 硬件栏:PP
- Bed dimensions:16×25 mm
- Cat. No.:GEHE17-5080-01
- pH 范围:2 - 9
- 存储:4 to 30 °C, 20% Ethanol
- 最大压力:5 bar
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