Growth Factor Reduced (GFR) formulation is well suited for applications that require a more highly defined basement membrane preparation.
- Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing
- Extensive PCR testing is performed to screen for a number of pathogens
- Tested and found negative for bacteria, fungi, and mycoplasma
- Protein concentrations determined by Lowry method
- Endotoxin units measured by Limulus Amoebocyte Lysate assay
- Matrigel Matrix gel stability tested for a period of 14 days at 37°C
- Biological activity determined for each lot using a neurite outgrowth assay.
Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors.
Application
• Cell Growth and Differentiation
• Metabolism/Toxicology Studies
• Invasion Assays
• In Vitro and In Vivo Angiogenesis Assays
• In Vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice
Preparation and Storage
Store at -20°C. Avoid multiple freeze-thaws
Note: For research use only. Not intended for diagnostic or therapeutic procedures.
When warmed, the solution polymerizes to produce a biologically active matrix material resembling the mammalian cellular basement membrane. It is ideal for use with in vitro cell cultures of a broad range of cell types, including both primary and stem cell applications.
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- Environmentally Preferable:Low Manufacturing Impact
- 环境认证:ISO 14001 - Bedford
- Source:Engelbreth-Holm-Swarm mouse tumour
- 质量控制:Tested for ability to gel quickly and maintain this form with culture medium for a period of 14 days at 37 °C
Tested for the ability to promote neurite outgrowth of chick dorsal root ganglia cells
Tested and found negative for bacteria, fungi, and mycoplasma
Tested for endotoxin (LAL assay) - 存储和稳定性:Stable for at least three months at −20 °C or 12 days at 37 °C
Cellular Analysis of 3D Spheroid-Based Tumor Invasion Assays Corning® Matrigel® Basement Membrane, Matrix for 3D Culture In Vitro Corning® Matrigel® Matrix, Frequently Asked Questions Culture and Assay Systems Used for 3D Cell Culture Methods for Implantation of Corning® Matrigel® Matrix into Mice and Tissue Fixation