Protein G Sepharose 4 Fast Flow has recombinant protein G immobilized by the cyanogen bromide (CNBr) method to Sepharose 4 Fast Flow.
- For antibody affinity chromatography
- Fractionation of monoclonal antibodies without binding albumin
- Recombinant protein G lacking albumin-binding region
- Binding specificities that complement Protein A media
- Binds a broad range of IgG species and subclasses
- Cyanogen bromide activation coupling method
- Multi-point attachment minimises ligand leakage
- Used in a range of research applications
As member of the BioProcess media range, Protein G Sepharose 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Protein G exhibit binding specificities that complement Protein A media and binds to the Fc region of IgG from a variety of mammalian species. Protein G Sepharose 4 Fast Flow may be used to isolate and purify classes, subclasses and fragments of immunoglobulins from any biological fluid or cell culture medium.
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- pH 范围:3 - 9
- 基质:Cross-linked agarose, 4%, spherical
- 配体:Recombinant protein G lacking albumin-binding region, produced in E. coli
- 存储温度:2 - 8 °C
- 配体密度:2 mg Protein G/ml
- 配体偶联法:Cyanogen bromide activation