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Specifications
- 包装规格:1 KIT
- 说明:Next generation cell free protein expression kit (Wheat germ)
- 存储温度:−70 °C
- No. of reactions:50 reactions
Specifications
About this item
Cell-free protein expression (CFPE) is the production of recombinant proteins in solution using biomolecular translation machinery extracted from cells of different species, such as E. Coli, wheat germ, Leishmania tarentolae, rabbit reticulocyte, insect or human cells. CFPE system from wheat germ extract is suitable for the expression of both prokaryotic and eukaryotic proteins.It usually does not require codon optimization even for the expression of transcripts with a high A/T or G/C content.
- Great Time and cost efficiency, easy to use (batch model)
- Excellent yield: 5,5 ml sample scale per kit brings 0,5 to 1 mg proteins to you in a day at low cost
- No cloning or subcloning necessary for full length or truncation
- Animal- and serum-free
- Of eukaryotic origin
- High solubility due to native conformation and eukaryotic source
- Greater stability
- Suitable for protein labeling and the expression of difficult-to-express proteins like membrane proteins and multiple protein complexes
Shipping condition - Dry ice
Technique - Protein expression: suitable
An eukaryotic cell free protein expression system (CFPE/CFPS) with high yield and excellent scalability.
CFPE system (wheat germ) provides best yield among the eukaryotic systems mentioned above. This system is particularly suitable for production of proteins with complicated conformation and multiple protein complexes, such as disulfide-bond-containing or integral membrane proteins. A variety of proteins and large proteins have been successfully produced from CFPE system (wheat germ), such as human or plant proteins or proteins for vaccine studies.
Components
This product is comprised of the following:
1× 25 mM NTP mix
1× T7 RNA polymerase
10× Transcription buffer
1× 100 mM DTT
1× Wheat germ extract
1× Amino acid mix
Storage and stability - Store the components at the designated temperatures once received
10× Transcription buffer: −20 °C
25 mM NTP mix: −20 °C
T7 RNA polymerase: −20 °C
100 mM DTT: −20 °C
Wheat germ extract: −70 °C
Amino acid mix: −70 °C