The CellTiter-Glo® Luminescent Cell Viability Assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, an indicator of metabolically active cells. It is designed for use with multiwell formats, making it ideal for automated high-throughput screening (HTS), cell proliferation and cytotoxicity assays.
- Homogeneous 'add-mix-measure' format reduces the number of steps
- Higher level of detection requires fewer cells per assay
- Process plates consecutively with a very stable luminescent signal
- Detects as few as 15 cells/well in a 384-well format in 10 minutes
The homogeneous 'add-mix-measure' format results in cell lysis and generation of a luminescent signal proportional to the amount of ATP present. The amount of ATP is directly proportional to the number of cells present in culture. The CellTiter-Glo® Assay generates a 'glow-type' luminescent signal, which has a half-life generally greater than five hours, depending on cell type and medium used. The extended half-life eliminates the need to use reagent injectors and provides flexibility for continuous or batch mode processing of multiple plates.