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Specifications
- 流速:<20 ml/min
- 颗粒大小:34 µm
- 最大压力:5 bar
- 容量:1×5 ml
- 说明:HiTrap™ IgY Purification HP
- 顺序:01
- pH 范围:3 - 11
- 硬件栏:PP
- Bed dimensions:16×25 mm
- 包装规格:1
Specifications
About this item
HiTrap IgY Purification HP is a 5 ml column prepacked with Sepharose High Performance for fast and easy purification of IgY from egg yolk.
- Each column binds IgY from a one-quarter portion of egg yolk (approx. 100 mg IgY). For greater capacity, several columns may be connected in series
- Packed with a thiophilic adsorption medium, the ligand, 2-mercaptopyridine is coupled to Sepharose High Performance
- Recovery and purity of IgY are excellent
- Can be operated with a liquid chromatography system such as ÄKTA design
- The top and bottom frits are manufactured from porous polyethylene
HiTrap IgY Purification HP is packed with a thiophilic adsorption medium, 2-mercaptopyridine coupled to Sepharose High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgY and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.