琼脂糖

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琼脂糖, Electran, high resolution, low melting for electrophoresis

Supplier: VWR Chemicals

Agarose high resolution is optimised to give exceptional separation on agarose of nucleic acid fragments below 1000 base pairs, and differing by only a few base pairs. It retains the low gelling characteristics of the standard Agarose HR, and offers significantly increased gel strength at the expense of a higher melting temperature. This results in gels which are flexible and easy to handle. These properties make it the agarose of choice for analytical gel separations to check the quality and size of amplified fragments, and for use in restriction mapping involving small digestion fragments.

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琼脂糖, Electran, high resolution for electrophoresis

Supplier: VWR Chemicals

Agarose high resolution (HR) has melting, max. 90 °C, and gelling temperatures (32,5 to 38 °C), but differs from traditional low melting point molecular biology grade agaroses in the gel separation range. Using this agarose in electrophoresis allows fine and consistent resolution of nucleic acids below 1000 base pairs, which differ by only a few base pairs. The properties of Agarose HR allow consistent gel separations (analytical and preparative) and performance of in-vitro translation and transcription mapping, as well as in-vivo ligation and transformation. For analytical gel separation of DNA/RNA below 1000 base pairs, where a higher gel strength for ease of manipulation is required, we recommend the use of Agarose, high resolution, Electran (43655).

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琼脂糖, II™ for biotechnology

Supplier: VWR Chemicals

A low melting agarose that melts between 62 to 68 °C and remains liquid for several hours at 37 °C. Ideal for purification of macromolecules from agarose slices and for in-gel enzymatic manipulations.