转染试剂
Avantor offers transfection reagents for numerous applications, including bioproduction, in vivo work, viral production, DNA and siRNA transfection, CRISPR/Cas 9 and more. While considering a suitable transfection reagent, it is important to identify the cell type and culture conditions. Specialized transfection reagents will address rare cell cultures, neurons, and primary cells are more difficult to manipulate for porous characteristics. Avantor also offers products for electroporation and the consumables needed to support the transfection workflow.
Transfection Reagents
Supplier: NANOENTEK
ADAMII assay slide are used in the cell type of thymocytes, splenocytes, PBMCs, dendritic cells, monocytes, lymphocytes, keratinocytes, epithelial cells, established cell lines, primary cells, stem cells, blood cells, neuronal cells.
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DNA transfection reagent, CombiMag™
Supplier: OZ BIOSCIENCES
CombiMag™ transfection reagent is based on the use of magnetic nanoparticles for improving transfection reagent efficiency. It has been designed to be employed in association with any commercial transfection reagent and can be used with all types of nucleic acids. It has been successfully tested on a broad range of primary cells, hard to transfect cells and cell lines.
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Transfection reagent, TransIT®-293
Supplier: MIRUS
TransIT®-293 transfection reagent is specifically optimised to provide exceptional transfection efficiency of plasmid DNA in HEK 293 and cell types of associated lineage.
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Transfection reagent, FuGENE® HD
Supplier: PROMEGA
FuGENE® HD transfection reagent has a non-liposomal formulation designed to transfect DNA into a wide variety of cell lines with high efficiency and low toxicity. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex. Additionally, the FuGENE® HD Transfection Reagent has been shown to support transfection in chemically defined media and does not contain any animal-derived components.
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Transfection reagent, ViaFect™
Supplier: PROMEGA
ViaFect™ transfection reagent is designed to transfect DNA into a wide variety of cell lines with high efficiency and low toxicity. ViaFect™ Transfection Reagent has been shown to transfect cell lines that are traditionally thought of as difficult to transfect (such as hematopoietic, primary and iPSC stem cell lines) with relatively high efficiency. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex.
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DNA transfection reagent for virus production, PEIpro®
Supplier: POLYPLUS
PEIpro® is a ready to use 1 mg/ml optimised linear polyethylenimine (PEI) reagent for the production of virus and recombinant proteins by Transient Gene Expression (TGE) in suspension-adapted mammalian cell lines cultivated in shaker flasks, platform shakers or stirred tank bioreactors.
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DNA transfection reagents for protein production, FectoPRO®
Supplier: POLYPLUS
The FectoPRO® Transfection Kit is specifically designed for enhanced Transient Gene Expression (TGE) in suspension CHO and HEK-293 cells in various serum-free media, using low DNA amount (<1 μg/ml of cell culture).
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in vivo nucleic acid delivery reagent, in vivo-jetPEI®
Supplier: POLYPLUS
in vivo-jetPEI® reagent provides versatile, reproducible and reliable delivery of all types of nucleic acids (DNA, siRNA, miRNA and more) in animal models, for functional studies and RNA interference experiments using various administration routes.